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Plasmid#125265PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gly42 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsert7D12 Gly42 nanobody
UseTagsHis-tagExpressionBacterialMutationPromotertrc promoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-ppiB
Plasmid#202482PurposeExpresses peptidyl-prolyl cis-trans isomerase (ppiB) after phosphae depletionDepositorInsertpeptidyl-prolyl cis-trans isomerase (ppiB)
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Amp-T7-GFPuv
Plasmid#202463PurposeExpresses GFPuv with T7 inductionDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-LCAmp-trxB
Plasmid#202465PurposeExpresses a guide RNA to silence trxB after phosphate depletionDepositorInserttrxB guide RNA
UseTagsExpressionBacterialMutationPromoterugpBAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-sfGFP(1-157)
Plasmid#220743PurposeGAL4-sfGFP(1-157) construct expression in mammlian cells.DepositorInsertGAL4-sfGFP(1-157)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-sfGFP(158-238)
Plasmid#220744PurposeGAL4-sfGFP(158-238) construct expression in mammlian cells.DepositorInsertGAL4-sfGFP(158-238)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-NIR-FbLAG30
Plasmid#220746PurposeGAL4-NIR-FbLAG30 construct expression in mammlian cells.DepositorInsertGAL4-NIR-FbLAG30
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbLAG16
Plasmid#220739PurposeNIR-FbLAG16 expression in mammalian cells.DepositorInsertNIR-FbLAG16
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbLAG30
Plasmid#220740PurposeNIR-FbLAG30 expression in mammalian cells.DepositorInsertNIR-FbLAG30
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb(718)
Plasmid#220742PurposeNIR-Fb(718) expression in mammalian cells.DepositorInsertNIR-Fb(718)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmEos2-N1 GBP (GBP-mEos2)
Plasmid#162876PurposeExpression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization MicroscopyDepositorInsertGFP Binding Protein tagged with mEos2
UseTagsmEos2ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_A8_His
Plasmid#201718PurposeExpression of nanobodyDepositorInsertVHH_A8 (S lama glama (llama))
UseTagsHexahistidineExpressionBacterialMutationPromoterAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_A10-5_His
Plasmid#201720PurposeExpression of nanobodyDepositorInsertVHH_A10-5 (S lama glama (llama))
UseTagsHexahistidineExpressionBacterialMutationPromoterAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_B5-5_His
Plasmid#201721PurposeExpression of nanobodyDepositorInsertVHH B5-5 (S lama glama (llama))
UseTagsHexahistidineExpressionBacterialMutationPromoterAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
NbAlfa-GFP2
Plasmid#201485PurposeBRET2 detection of ALFA-tagged proteinDepositorInsertNbAlfa-GFP2
UseTagsGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP2-NbALFA
Plasmid#201486PurposeBRET2 detection of ALFA-tagged proteinDepositorInsertGFP2-NbALFA
UseTagsGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
NbV5-LgBit
Plasmid#201475PurposeNanoBit detection of V5-tagged proteinDepositorInsertNbV5-LgBit
UseTagsLgBitExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-NbGFP
Plasmid#166858PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.DepositorInsertcsgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_LaG16_2_synNotch_Gal4VP64
Plasmid#85421PurposeLentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic NotchDepositorInsertLaG16_2 GFP nanobody synNotch Gal4VP64
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing the MBP sybody Sb_MBP#1
Plasmid#132699Purposeexpresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISADepositorInsertsybody_MBP#1
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only