We narrowed to 59,537 results for: cloning

1. pOPING

   Plasmid

   #26046

   Depositor

   Ray Owens

   Article

   Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

   Insert

   SS peptide-LacZ-KHHHHHH
   

   Tags

   KHHHHHH and MGILPSPGMPALLSLVSLLSVLLMGCVAETG

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. BASIC_8_RBS34- mCherry -B0015

   Plasmid

   #68142

   Purpose

   storage vector for BASIC part: RBS-RFP(ORF)

   Depositor

   Geoffrey Baldwin

   Article

   Storch et al ACS Synth Biol. 2015 Apr 16.

   Insert

   RBS34-mcherry-B0015
   

   Expression

   Bacterial

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHACK-GAL4>QF2(newHA1)

   Plasmid

   #104873

   Purpose

   HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   T2A-QF2
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB700 lenti gRNA mcherry

   Plasmid

   #167910

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-Gal4

   Plasmid

   #53366

   Purpose

   full-length native Gal4 with stop in Gateway middle entry clone

   Depositor

   Joshua Bonkowsky

   Article

   Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

   Insert

   native (full-length) Gal4
   

   Promoter

   no promoter

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. p663-UBC-V5-CDK13_IDG-K

   Plasmid

   #135268

   Purpose

   Gateway destination clone of CDK13 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   V5-CDK13 (CDK13 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB700 lenti gRNA EYFP

   Plasmid

   #167906

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p221z-CAS9p-t35s

   Plasmid

   #118385

   Purpose

   Entry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   CAS9p
   

   Use

   CRISPR

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p663-UBC-miniTurbo-V5-MAPK15_IDG-K

   Plasmid

   #170581

   Purpose

   Gateway destination clone of MAPK15 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-MAPK15 (MAPK15 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p667-UBC-BCKDK-V5-miniTurbo_IDG-K

    Plasmid

    #158189

    Purpose

    Gateway destination clone of BCKDK (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    BCKDK-V5-miniTurbo (BCKDK Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-HA-hTAF5

    Plasmid

    #17984

    Depositor

    Naoko Tanese

    Article

    Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

    Insert

    TAF5 (TAF5 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

12. p663-UBC-miniTurbo-V5-MARK1_IDG-K

    Plasmid

    #170582

    Purpose

    Gateway destination clone of MARK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-MARK1 (MARK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Human p150,95

    Plasmid

    #8633

    Depositor

    Timothy Springer

    Article

    Corbi et al EMBO J 1987 Dec 20;6(13):4023-8.

    Insert

    Human p150,95 (ITGAX Human)
    

    Expression

    Mammalian

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRATIO4212

    Plasmid

    #141313

    Purpose

    Gateway cloning compatible dual fluorescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Tags

    mScarlet-I and mVenus

    Expression

    Plant

    Promoter

    UBQ10 promoter

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB700 lenti gRNA Blasto_zhang2.0

    Plasmid

    #167912

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. OgeuIscB ωRNA-v13

    Plasmid

    #219634

    Purpose

    human U6-driven expression of OgeuIscB omegaRNA-v13 (ωRNA-v13) scaffold with BsmBI for guide cloning

    Depositor

    Weixin Tang

    Article

    Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

    Insert

    OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHPyV7-713a

    Plasmid

    #24728

    Depositor

    Christopher Buck

    Article

    Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

    Insert

    Full genome of Human polyomavirus 7
    

    Use

    Viral clone

    Available Since

    June 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-FlEx switch

    Plasmid

    #82406

    Purpose

    Gateway-compatible FlEx cassette containing two pairs of heterotypic, antiparallel loxP-type recombination sites

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Type

    Empty backbone

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. p663-UBC-miniTurbo-V5-CDK15_IDG-K

    Plasmid

    #158179

    Purpose

    Gateway destination clone of CDK15 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK15 (CDK15 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICH41414

    Plasmid

    #50337

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    3'UTR, polyadenylation signal/terminator, 35s (Cauliflower Mosaic Virus)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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