We narrowed to 11,578 results for: VARS

1. GLuc-PM

   Plasmid

   #164783

   Purpose

   Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

   Depositor

   Laszlo Hunyady

   Article

   Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

   Insert

   Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Gaussia princeps, Human)
   

   Use

   Tags

   GLuc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETcon_SARS-CoV-2_RBD

   Plasmid

   #166782

   Purpose

   Yeast surface display vector, expressing the SARS-2 RBD, 201aa construct

   Depositor

   Jesse Bloom

   Article

   Starr et al Cell. 2020 Sep 3;182(5):1295-1310.e20. d

   Insert

   Spike receptor binding domain (RBD) from SARS-CoV-2 (S )
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GB1-A11(2-196, D40G)-Strep

   Plasmid

   #196210

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Use

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   D40G

   Promoter

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GB1-A11(2-196, G38R)-Strep

   Plasmid

   #196209

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Use

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   G38R

   Promoter

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3_SP-GLP1R-V2R-NTEV-TCS-GV-2xHA

   Plasmid

   #194381

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   SP-GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Synthetic, Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. METTL7A pEGFP-N2 (890)

   Plasmid

   #62017

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   METTL7A (METTL7A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pmCherry C1 Omp25

   Plasmid

   #157758

   Purpose

   Expression of mcherry-labelled mitochondrial outer membrane protein 25 in cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   SYNJ2bp (SYNJ2BP Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXS-IRES-Blast POLA1

   Plasmid

   #160807

   Purpose

   Express POLA1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLA1 (POLA1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Codon Optimized

   Promoter

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57.1 DOX off blast NFS1

   Plasmid

   #160801

   Purpose

   Express Dox repressible NFS1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   NFS1 (NFS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

    Plasmid

    #216477

    Purpose

    lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    dsRed
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1aL and UBC

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-G-AsCas12a crRNA-BsaI cassette (MSP3491)

    Plasmid

    #114067

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of AsCas12a guides

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for AsCas12a crRNAs
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. HDM_Spikedelta21_sinobiological_B.1.617.2

    Plasmid

    #204149

    Purpose

    Expression plasmid for SARS-CoV-2 Delta variant spike

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    SARS-CoV-2 Delta variant spike (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    21 amino acid cytoplasmic tail deletion

    Promoter

    CMV

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiU6-hIL2RN gRNA_multi1-3-MS2-Puro

    Plasmid

    #192684

    Purpose

    Lentiviral expression of multi gRNAs targeting hIL1RN promoter to activate human IL1RN transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human IL1RN activating gRNAs #1,2,3 (IL1RN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmCherry N1 MFN2

    Plasmid

    #157759

    Purpose

    Expression of MFN2:mcherry in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    MFN2 (MFN2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GB1-A11(2-196, G175R)-Strep

    Plasmid

    #196211

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G175R

    Promoter

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GB1-A11(2-196, G189E)-Strep

    Plasmid

    #196212

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G189E

    Promoter

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SUV[SET]-dCas9

    Plasmid

    #100088

    Purpose

    Catalytic domain [SET] of human SUVAR39H1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    SUVAR39H1 (SUV39H1 Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-SUV[SET]-dCas9-NLS

    Expression

    Mammalian

    Mutation

    deleted aa 1-76

    Promoter

    CMV

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNMSB104

    Plasmid

    #199314

    Purpose

    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mTagBFP2 from pJJR81, C. elegans codon optimized …

    Promoter

    flp-18

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGES401

    Plasmid

    #190198

    Purpose

    For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection

    Depositor

    Yuefeng Guan

    Article

    Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    3xFlag

    Expression

    Plant

    Mutation

    plant-codon optimized

    Promoter

    Glycine max elongation factor 1A(pM4)

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-eNme2-T.2-ABE8e

    Plasmid

    #185669

    Purpose

    Expresses eNme2-T.2-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-T.2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

    Promoter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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