We narrowed to 80,372 results for: TRI

1. pAc5.1B-lambdaN-HA-DmEDC3-ADF_E

   Plasmid

   #146195

   Purpose

   Insect Expression of DmEDC3-ADF

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   DmEDC3-ADF (Edc3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation A456G and three mutations N16…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-lambdaN-HA-DmEDC3-FAF_E

   Plasmid

   #146199

   Purpose

   Insect Expression of DmEDC3-FAF

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   DmEDC3-FAF (Edc3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation A456G and three mutations N16…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-lambdaN-HA-DmEDC3-FDA_E

   Plasmid

   #146200

   Purpose

   Insect Expression of DmEDC3-FDA

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   DmEDC3-FDA (Edc3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation A456G and three mutations N16…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTij_sg_mmu_Med1_N_terminal

   Plasmid

   #197870

   Purpose

   CRISPR/Cas9/sgRNA plasmid for cutting Med1 N terminal and building mEmerald/Halo-MED1 knock-in mESCs

   Depositor

   James Zhe Liu

   Article

   Dong et al bioRxiv 2022.10.26.513917

   Insert

   CRSP210 (Med1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR4 Ta-SRO1 JN177

   Plasmid

   #192546

   Purpose

   pENTR plasmid Triticum aestivum SRO1, full CDS, cultivar JN177, no stop codon

   Depositor

   Lennart Wirthmueller

   Article

   Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

   Insert

   SIMILAR TO RCD ONE 1 (SRO1), cultivar Jinan 177 (JN177)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR4 Ta-sro1 SR3

   Plasmid

   #192547

   Purpose

   pENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no stop codon

   Depositor

   Lennart Wirthmueller

   Article

   Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

   Insert

   SIMILAR TO RCD ONE 1 (SRO1), cultivar Shanrong No. 3 (SR3)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pWPT-mEGFP

   Plasmid

   #190606

   Purpose

   Obtained by creating a deletion inside the mCherry coding sequence in pWPT-/GCCACC-mEGFP-IRES-mCherry (Addgene #49235)

   Depositor

   Alessandro Quattrone

   Article

   Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   a deletion was created in mCherry CDS impairing i…

   Promoter

   EIF1-short

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA 3.1 BS (-) N protein

   Plasmid

   #188448

   Purpose

   SARS CoV-2 N protein expression

   Depositor

   Ajit Chande

   Article

   Mishra et al Life Sci Alliance. 2022 Mar 16;5(7). pii

   Insert

   SARS-CoV-2 N protein
   

   Use

   Tags

   Strep-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPR2_+10D+7U

   Plasmid

   #149393

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   7bp inserted upstream, and 10bp inserted downstre…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRISPR2_+10D+10U

    Plasmid

    #149394

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    10bp inserted upstream and downstream of the IHFp…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPR2_IHF_Mut

    Plasmid

    #149396

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    IHFprox site is mutated at key positions needed f…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEM-T Easy-Cas9-nls-CDS1

    Plasmid

    #160231

    Purpose

    Cas9-nuclear localization, level 0 of MoClo Golden Gate position CDS1

    Depositor

    Matias Kirst

    Article

    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

    Insert

    Cas9-nuclear localization signal gene sequence, Golden Gate MoClo position CDS1
    

    Use

    Level 0 of moclo golden gate position cds1

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pST_B4YSF1_9LAMI (pBS0347)

    Plasmid

    #185186

    Purpose

    For the mammalian expression of the Dorcoceras hygrometricum protein B4YSF1_9LAMI. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    B4YSF1_9LAMI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVH666

    Plasmid

    #169610

    Purpose

    Tier-2 vector encoding PTtgO2-driven SEAP-p2A-iRFP670, PmPGK1-driven TtgA and PmPGK1-driven mRuby2 expression (PTtgO2-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-TtgA-pA::PmPGK1-mRuby2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP and iRFP expression, PPGK-driven TtgA expression cassette and PPGK-driven YPet expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO7-PCMVmin / PPGK / PPGK

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVH664

    Plasmid

    #169608

    Purpose

    Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven rtTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::PmPGK1-YPet-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP and iRFP expression, PPGK-driven rtTA expression cassette and PPGK-driven YPet expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO7-PCMVmin / PPGK / PPGK

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTS2307-Tier1-2xcHS4-PPGK-NLuc_TB

    Plasmid

    #169550

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    cHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

    Plasmid

    #169597

    Purpose

    Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9 - VP64 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro

    Plasmid

    #169665

    Purpose

    Tier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK / RPBSA

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTS2333_Tier3(Lenti_inverse)-YPet_Puro

    Plasmid

    #169664

    Purpose

    Tier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven YPet and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / RPBSA

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVH663

    Plasmid

    #169607

    Purpose

    Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven tTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-tTA-pA::PmPGK1-YPet-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP and iRFP expression, PPGK-driven tTA expression cassette and PPGK-driven YPet expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO7-PCMVmin / PPGK / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge