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pOBCol3.6-GFPtpz

Plasmid

#110211

Purpose

Labels immature osteoblasts and connective tissue cells expressing type 1 collagen.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Insert

eGFPtopaz

Tags

None

Expression

Mammalian

Mutation

None

Promoter

3.6 kb rat type 1 collagen + first intron

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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PB-EF1-IRES-NEO mutant ITR

Plasmid

#67905

Purpose

PiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutation

Depositor

Alex Kentsis

Article

Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

Insert

NeoR (neoR Synthetic)

Expression

Mammalian

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_human-4xUAS

Plasmid

#125150

Purpose

Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

4 x upstream activating sequence (UAS)

Use

Stap-seq screening vector

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX_970_puro_DEST_V5_TLOC1 (399aa)

Plasmid

#45288

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TLOC1 (SEC62 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
RCAS DNhRARalpha (CC#1865)

Plasmid

#15153

Depositor

Connie Cepko

Article

Sen et al Development. 2005 Dec . 132(23):5147-59.

Insert

RAR alpha (dom neg) (RARA Human)

Use

Retroviral; Avian expression

Mutation

Dominant negative. Truncated at aa403, so lacks t…

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only
Flag Snail 2SA

Plasmid

#16219

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

Flag

Expression

Mammalian

Mutation

2SA: S97A, S101A

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pRK5 CASTOR2-HA Cterm

Plasmid

#84489

Purpose

overexpression of CASTOR2 with HA tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 (GATSL2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pBEAST-pBen-Luc

Plasmid

#122694

Purpose

Output expression of firefly luciferase under the activation of BenR transcription factor for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

Firefly luciferase

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE bleo human IRS-1

Plasmid

#11448

Depositor

Ronald Kahn

Article

Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

Insert

IRS-1 (IRS1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pETconNK-TEM1(S70A,D179G)

Plasmid

#81166

Purpose

pETconNK with TEM1(S70A,D179G) beta-lactamase

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

TEM-1 beta-lactamase

Tags

c-myc

Expression

Bacterial and Yeast

Mutation

Deleted the sec transport tag. Plasmid encodes H2…

Promoter

GAL1 and gal1

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG36-ChAT

Plasmid

#99069

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea ChAT (choline acetyltransferase)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

ChAT (choline acetyltransferase) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE puro mouse IRS-2

Plasmid

#11371

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-2 (Irs2 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
sgRNA3_GAL4UAS-Luciferase reporter

Plasmid

#64159

Purpose

Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA3 for GAL4UAS-Luciferase reporter

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pBEAST-pBen-sfGFP

Plasmid

#114598

Purpose

"Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

PBen

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBT305_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PL-FRT5

Plasmid

#52539

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pGL2-100bp

Plasmid

#16294

Depositor

Wafik El-Deiry

Article

Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

Insert

p53 promoter fragment (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

fragment -88 to +12 relative to first transcripti…

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pBEAST-BenR

Plasmid

#114597

Purpose

"Strong constitutive expression of transcription factor, BenR, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

BenR

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

OR2-OR1-Pr

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

Plasmid

#125225

Purpose

Expresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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CD200RCd4d3+4-bio

Plasmid

#32406

Purpose

A positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200 receptor) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200 Receptor extracellular domain (Cd200r1 Rat)

Tags

Cd4d3+4 and biotinylation peptide

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pGLTR-FP-GFP

Plasmid

#58242

Purpose

GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven eGFP marker

Depositor

Stephan Geley

Article

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Insert

attR1-Cm(R)-CcdB-attR2

Use

Lentiviral and RNAi; Gateway destionation vector

Expression

Mammalian

Promoter

no

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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