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pGEMT-PT2A-GFP-Tdtomato-iRFP670

Plasmid

#111812

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Publication

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

GFP
Tdtomato
iRFP670

Use

Cloning intermediate

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPA-RL-FL

Plasmid

#78863

Purpose

DULIP Tandem Construct used for data normalization

Depositor

Erich Wanker

Publication

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

Firefly

Use

Expression vector

Tags

Protein A, Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pRZ-mNanog-YNL

Plasmid

#65717

Purpose

mNanog reporter plasmid (expresses YNL)

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

murine Nanog promoter and YNL

Use

Lentiviral and Luciferase

Available since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB7

Plasmid

#63108

Purpose

Expression of HSPB7 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Publication

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hspb7 (HSPB7 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-CR033

Plasmid

#234042

Purpose

expresses ABE8e(V106W) base editor in a human T cell optimized lentiviral transfer plasmid

Depositor

Alexander Marson

Publication

Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

Insert

TadA8e(V106W)-Cas9(D10A)-P2A-blast

Use

Lentiviral

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-SUMO-KaiA

Plasmid

#68049

Purpose

Strain: AM5115, Used for protein purification

Depositor

Susan Golden

Publication

Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

Insert

KaiA

Tags

6xHis and SUMO

Expression

Bacterial

Promoter

T7

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Mouse ZP1 (JD#264)

Plasmid

#14644

Depositor

Jurrien Dean

Publication

Epifano et al Development. 1995 Jul . 121(7):1947-56.

Insert

ZP1 (Zp1 Mouse)

Expression

Bacterial

Available since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HA-HIF2α P405A Δ450-572-pBabe-Puro

Plasmid

#25958

Depositor

William Kaelin

Publication

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pME-zFUCCI (JDW 1464)

Plasmid

#242558

Purpose

Gateway middle entry clone containing zebrafish FUCCI reporter; For visualizing cell cycle state.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)

Use

Gateway Cloning

Available since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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FSW-ALDH2-T2A-EGFP

Plasmid

#197036

Purpose

Express ALDH2 from hSyn1 promoter, co-expressed with EGFP

Depositor

Ronald Hart

Publication

Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

Insert

ALDH2 (ALDH2 Human)

Use

Lentiviral

Expression

Mammalian

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-ECC-Biosensor

Plasmid

#140832

Purpose

Encoding the CAG promoter based eccDNA biosensor

Depositor

Yonglun Luo

Publication

Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ECC-Biosensor

Use

Piggybac (pb) transposon

Expression

Mammalian

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_MITF_P2A_Hygro_Barcode

Plasmid

#120460

Purpose

Barcoded lentiviral vector to express MITF in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MITF (MITF Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LbCpf1-U6_FlipArray

Plasmid

#109349

Purpose

Lentiviral vector to express Cpf1 from EFS promoter, as well as a Cpf1 FlipArray from U6 promoter.

Depositor

Sidi Chen

Publication

Chow et al Nat Commun. 2018 May 15;9(1):1903. doi:

Insert

U6-FlipArray

Use

CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

Expression

Mammalian

Promoter

U6

Available since

July 14, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-YAP1 CA

Plasmid

#79502

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDS

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

YAP1 (YAP1 Human)

Use

Gateway Cloning

Tags

3XFLAG

Mutation

S127A

Promoter

no Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-human MIZ1 wild type

Plasmid

#74161

Purpose

bacterial expression of MIZ1

Depositor

Martine Roussel

Publication

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

MIZ1 (ZBTB17 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMB-AUTr

Plasmid

#61205

Purpose

Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LC3(ATG8)

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe hygro MTH1

Plasmid

#21296

Depositor

Bob Weinberg

Publication

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

MTH1 (NUDT1 Human)

Use

Retroviral

Expression

Mammalian

Available since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pBS/KS-mId4sp

Plasmid

#16046

Depositor

Robert Benezra

Publication

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id4 (Id4 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 665-945

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
AiP12828 - pAAV-AiE0456m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2828) (AAV8)

Viral prep

#224006-AAV8

Purpose

Ready-to-use AAV8 particles produced from AiP12828 - pAAV-AiE0456m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2828) (#224006). In addition to the viral particles, you will also receive purified AiP12828 - pAAV-AiE0456m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2828) plasmid DNA. minBG-driven expression of SYFP2 in cortical L5_ET neurons. These AAV preparations are suitable purity for injection into animals.

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Promoter

minBG

Available since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only
pHS9_10xHis_MBP_TEV_dCas9

Plasmid

#244831

Purpose

Bacterial expression of catalytically inactive Cas9 (dCas9) for protein purification

Depositor

Jennifer Doudna

Publication

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dCas9 (WT)

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D10A/H840A

Promoter

T7

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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