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pcDNA 3.1 myc-Rab10

Plasmid

#208366

Purpose

Mammalian expression of myc-tagged human Rab10

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

myc-Rab10 (RAB10 Human)

Expression

Mammalian

Mutation

wild type

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/20]

Plasmid

#206744

Purpose

Mammalian Expression of GFP scFV. Derived from hybridoma N86/20 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GFP (Aequorea victoria) recombinant scFV

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2_1-180_AE

Plasmid

#148578

Purpose

Mammalian Expression of HsGIGYF2_1-180

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180 (GIGYF2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pPbcas13b

Plasmid

#89906

Purpose

Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

Depositor

Feng Zhang

Article

Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

Insert

Cas13b

Expression

Bacterial

Promoter

Lac

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/44]

Plasmid

#206743

Purpose

Mammalian Expression of GFP scFV. Derived from hybridoma N86/44 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GFP (Aequorea victoria) recombinant scFV

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF17_P2A_Hygro_Barcode

Plasmid

#120503

Purpose

Barcoded lentiviral vector to express KLF17 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF17 (KLF17 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer

Plasmid

#176588

Purpose

Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

CRISPR-associate IscB (Delaware Bay) locus

Expression

Bacterial

Mutation

Replaced spacer in CRISPR array with Fn spacer

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES2-mCherry-p53 deltaN

Plasmid

#49243

Purpose

expresses human p53 deltaN and mCherry in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 deltaN (TP53 Human)

Tags

IRES-mCherry

Expression

Mammalian

Mutation

deltaN ( lacks 39 residues at the N-terminus)

Promoter

CMV

Available Since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

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CLK1

Plasmid

#155458

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CLK1 (CLK1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJT302 Ec03 Lenti pEF-attB LSR GFP

Plasmid

#184945

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Ec03 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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EXT1- D164A-pLJC2-3XFLAG

Plasmid

#215828

Purpose

Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

Depositor

Yoav Shaul

Article

Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

Exostosin glycosyltransferase 1 Mutant (EXT1 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a_ESRRG_P2A_Hygro_Barcode

Plasmid

#120435

Purpose

Barcoded lentiviral vector to express ESRRG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ESRRG (ESRRG Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJT303 Ec04 Lenti pEF-attB LSR GFP

Plasmid

#184946

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Ec04 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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MBP-CHC(1-1074)-His6

Plasmid

#100748

Purpose

Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CLTC (CLTC Human)

Tags

His and MBP

Expression

Bacterial

Mutation

Residues 1-1074

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_LMX1A_P2A_Hygro_Barcode

Plasmid

#120457

Purpose

Barcoded lentiviral vector to express LMX1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

LMX1A (LMX1A Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro

Plasmid

#170317

Purpose

Expresses HA and EGFP tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/8]

Plasmid

#199429

Purpose

Mammalian expression of GFP scFv. Derived from hybridoma N86/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingGFP (Aequorea victoria)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Integrin beta5-2XEGFP

Plasmid

#139779

Purpose

Detecting Integrin beta5 by fluorescence microscopy

Depositor

Staffan Stromblad

Article

Lock et al Nat Cell Biol. 2018 Nov;20(11):1290-1302

Insert

Integrin subunit beta5 (ITGB5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-LTR-dCas9-p65AD-BFP

Plasmid

#46913

Purpose

Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

dCas9-p65AD-BFP fusion
Puromycin resistance

Use

CRISPR and Retroviral

Tags

2xNLS, BFP, and VP64 domain

Expression

Mammalian

Promoter

LTR and PGK

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pJT304 Ec07 Lenti pEF-attB LSR GFP

Plasmid

#184947

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Ec07 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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