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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,038 results for: LEA

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Showing: 5481 - 5500 of 18038 results

  2. pCS2+ cMyc Dam_f_GFPoNLS

    Plasmid
    #85820
    Purpose
    iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Publication
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    cMyc Dam_f_GFPoNLS
    Tags
    cMyc and oNLS (optimized nuclear localization sig…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A.
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85820-plasmid-map-sequence-id-170474

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  3. pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L

    Plasmid
    #52478
    Purpose
    Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T
    Depositor
    Sebastian Seung
    Publication
    Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    EGFP
    nuclear mTagBFP
    Synaptophysin-TagRFP-T
    Use
    Rabies virus
    Tags
    TagRFP-T
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    52478-plasmid-map-sequence-id-81361

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  9. pRSET.HaloTag.v7.AviTag

    Plasmid
    #195486
    Purpose
    Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.
    Depositor
    Tim Brown, HHMI-JRC Tool Translation Team
    Insert
    HaloTag.v7.AviTag
    Tags
    6xHIS and AviTag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    195486-plasmid-map-sequence-id-393193

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  10. Membrane EGFP

    Plasmid
    #198056
    Purpose
    Plasma membrane localizing EGFP using Human LCK proto-oncogene sequence
    Depositor
    Amro Hamdoun
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LCK membrane EGFP (LCK A. victoria, Synthetic, Human)
    Use
    Sea urchin, mammal, zebrafish
    Tags
    EGFP
    Mutation
    Tandem repeat of N-terminal LCK membrane targetin…
    Promoter
    sp6
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    198056-plasmid-map-sequence-id-389155

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  12. pGBW-m4046814

    Plasmid
    #145601
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145601-plasmid-map-sequence-id-292512

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  14. Human 3' HP1a AID GFP PuroR

    Plasmid
    #127906
    Purpose
    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a Gene
    Depositor
    Mark Groudine
    Publication
    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HP1 (CBX5 Mustard Weed, Human)
    Use
    Donor plasmid
    Tags
    GFP, AID, PuroR
    Mutation
    Inserting GFP AID 2A Puro into mouse 3' Hp1a
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127906-plasmid-map-sequence-id-250992

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  18. pGBW-m4046921

    Plasmid
    #145755
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145755-plasmid-map-sequence-id-292520

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  19. FKBP-Dronpa

    Plasmid
    #87709
    Purpose
    FKBP-tagged Dronpa.
    Depositor
    Jin Zhang
    Publication
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP-Dronpa
    Tags
    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87709-plasmid-map-sequence-id-176199

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Showing: 5481 - 5500 of 18038 results