We narrowed to 10,133 results for: ADA

1. Circular 200,100 with interspersed loops (FANCC)

   Plasmid

   #180192

   Purpose

   AAV vector carrying a guide RNA targeting the human FANCC mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with interspersed loops
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Circular 200,100 with interspersed loops (CTNNB1)

   Plasmid

   #180193

   Purpose

   AAV vector carrying a guide RNA targeting the human CTNNB1 mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with interspersed loops
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNH605-PACE-YFP

   Plasmid

   #172002

   Purpose

   Fluorescent reporter for Rpn4 activity

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   4xPACE-YFP
   

   Use

   Vector for genomic integration into the yeast gen…

   Expression

   Yeast

   Promoter

   CYC1 core promotor with 4 tandem repeats of Rpn4 …

   Available Since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV_UdgX-APOBEC1-UdgX-nCas9

   Plasmid

   #163555

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-APOBEC1-UdgX-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Fireworks RFP[PTC+] (AVA2626)

   Plasmid

   #85444

   Purpose

   Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.

   Depositor

   Joan Steitz

   Article

   Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

   Insert

   tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
   

   Use

   Minimal backbone fragment for low-copy bacterial …

   Expression

   Mammalian

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_UdgX-APOBEC1-UdgX-HF-nCas9

   Plasmid

   #163559

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-APOBEC1-UdgX-HF-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. XIAP (pBS0852)

   Plasmid

   #185282

   Purpose

   For the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   XIAP (XIAP Human)
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. cacna2d1 HA pCAGGS

   Plasmid

   #206107

   Purpose

   expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549

   Depositor

   Annette Dolphin

   Article

   Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV/B-actin

   Available Since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. aav-tnt-actn2-gfp

   Plasmid

   #165034

   Purpose

   AAV-based delivery of ACTN2-GFP into cardiomyocytes in vivo

   Depositor

   William Pu

   Article

   Guo et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

   Insert

   ACTN2 (Actn2 Mouse)
   

   Use

   AAV

   Tags

   GFP

   Promoter

   cTNT

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMonAID_nCas9-PmCDA_Hyg_ALS

    Plasmid

    #91693

    Purpose

    Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A for nickase Cas9

    Promoter

    2x35S

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Raichu-OsRac1 WT

    Plasmid

    #133262

    Purpose

    Biosensor for monitoring activation for rice small GTPase OsRac1

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1 (LOC4325879 Oryza sativa)
    

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Promoter

    Ubi

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV.CBA.YFP.miR-E_shControl

    Plasmid

    #180386

    Purpose

    Producing AAV that encodes negative control shRNA with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    shControl
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNAD1

    Plasmid

    #154347

    Purpose

    pUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistance

    Depositor

    John van der Oost

    Article

    Naduthodi et al Biotechnol Biofuels. 2019 Mar 25;12:66.

    Insert

    ShBle
    

    Use

    Microalgae

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Violaxanthin Chlorophyll a Binding Protein Precur…

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIYN3

    Plasmid

    #197099

    Purpose

    To express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.

    Depositor

    Kensaku Sakamoto

    Article

    Mukai et al Biochem Biophys Res Commun. 2011 Aug 12;

    Insert

    TyrRS variant, Nap3 tRNA
    

    Expression

    Bacterial

    Mutation

    Ala70, Thr158, Ser159, Arg286 (TyrRS)

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. scFv-GCN4-DNMT3a-DNMT3l

    Plasmid

    #154140

    Purpose

    Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. hnRNPA2_RRM2

    Plasmid

    #157866

    Purpose

    express His tagged hnRNPA2 RRM2

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-TRE-flex-Clover

    Plasmid

    #135177

    Purpose

    AAV expression of TRE(TRE3G)-driven, Cre-dependent, Clover expression for fluorescent labling

    Depositor

    Tetsuo Yamamori

    Article

    Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116

    Inserts

    Clover
    TRE3G
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    a variant of GFP

    Promoter

    TRE3G

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGM

    Plasmid

    #71250

    Purpose

    luciferase reporter with human GM-CSF promoter -627 to + 28

    Depositor

    Peter Cockerill

    Article

    Cockerill et al Proc Natl Acad Sci U S A. 1999 Dec 21;96

    Insert

    GM-CSF promoter -627 to +28 fragment (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    GM-CSF

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-MCS-eDHFR(69K6)-EGFP

    Plasmid

    #172101

    Purpose

    Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    22aa-eDHFR(69K6)-EGFP
    

    Expression

    Mammalian

    Mutation

    eDHFR(69K6): Hexalysine (K6) sequence is inserted…

    Promoter

    CMV

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO5d.SFFV.eGFP-miR30n

    Plasmid

    #90333

    Purpose

    miR30 based shMir knockdown

    Depositor

    Dirk Heckl

    Article

    Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

    Inserts

    eGFP
    na
    SFFV
    miR30n
    

    Use

    Lentiviral

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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