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Plasmid#62970PurposeExpresses HA-tagged Gli1DepositorInsertGLI1 (GLI1 Human)
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEJS887_pTetR-P2A-BFPnls/sgAlpha
Plasmid#108651PurposeAlpha satellite repeats targeting Spy sgRNA under U6 promoterDepositorInsertsAlpha satellite repeats targeting sgRNA
TetR-P2A-BFP
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterU6 and hPGKAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEZT-GST-SLFN2
Plasmid#174319PurposeExpression of recombinant GST-SLFN2DepositorInsertGST-SLFN2 (Slfn2 Mouse)
UseBacmam, baculovirusTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCl-HA-YAP
Plasmid#27007DepositorInsertYAP (YAP1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-CHIKV-nsp3-c3xFLAG
Plasmid#215696PurposeProduces the Chikungunya virus nonstructural protein 3 with a c-terminal 3xFLAG tagDepositorInsertCHIKV nsp3
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
AM77_pU6.Sa-gRNA.CLYBL
Plasmid#199237PurposeExpression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locusDepositorInsertS. aureus gRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNAAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Nucbow
Plasmid#158992PurposeMulticolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
UseTagsAll FPs are fused to H2BExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK/CMV)-GtwyA
Plasmid#208574PurposeLV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmnucTomato
HSVtk
Neomycin
d2nucEGFP
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGK/CMV and minPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-STChRger2-TS-EYFP
Plasmid#129393PurposeSoma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertsoma targeted ChRger2
UseAAVTagsKv2.1-TS-EYFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1
Plasmid#107407PurposeLentiviral expression of gRNA resistant PBRM1DepositorInsertsg resistant PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationsgRNA resistantPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-P2A-BlastR
Plasmid#98147PurposeExpresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.DepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsP2A-BlastR fusion with Cas9-NluxExpressionMutationPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-ExRai-AktAR2
Plasmid#184048PurposeCytosol-targeted ExRai-AktAR2.DepositorInsertCyto-ExRai-AktAR2
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ad-Flag-XBP1s
Plasmid#63678PurposeAdenoviral vector for XBP1s expressionDepositorInsertXBP1 (XBP1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationsplicedPromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
xCas9
Plasmid#138565PurposeExpresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSDDepositorInsertxCas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki-67(RASA)-mCherry_IRESpuro2
Plasmid#182921PurposeExpresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.DepositorInsertMKI67 (MKI67 Human)
UseTagsEGFP and mCherryExpressionBacterial and MammalianMutationRASA mutationPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABEPuro-HA-GLI1
Plasmid#62967PurposeExpresses HA-tagged Gli1DepositorInsertGLI1 (GLI1 Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-VCPIP1
Plasmid#85671PurposeExpression of VCPIP1 with N-terminal GFP tagDepositorInsertVCPIP1 (VCPIP1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
SiC-V2-Cas9G7
Plasmid#133043PurposeDoxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.DepositorInsertTet repressor
UseLentiviralTagsCeruleanExpressionMutationPromoterAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
SuExp-hPLD4
Plasmid#201244Purposeexpress full length human PLD4 proteinDepositorInsertPLD4 (PLD4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
myristoylated ct-GRK2
Plasmid#178734PurposeN-myristoylation signaling peptide of Src and the C-terminal fragment of G-protein coupled receptor kinase 2 (ct-GRK2)DepositorInsertGRK2 (GRK2 Bovine)
UseLentiviralTagsSrc 1-15ExpressionMutationonly amino acids 548-671PromoterUbiquitinAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only