169,069 results

1. MSCV PIG Pu.1

   Plasmid

   #66982

   Purpose

   Retroviral expression plasmid for mouse Pu.1 expression; containing bicistronic IRES-GFP

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   Spi1 (Spi1 Mouse)
   

   Use

   Retroviral

   Promoter

   LTR

   Available Since

   July 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-syn-FLEX-splitTVA-EGFP-tTA (AAV1)

   Viral Prep

   #100798-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-syn-FLEX-splitTVA-EGFP-tTA (#100798). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-splitTVA-EGFP-tTA plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene#100799). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ian Wickersham

   Article

   Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

   Promoter

   Syn

   Tags

   EGFP (Cre-dependent)

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

3. Venus-rab7a

   Plasmid

   #234743

   Purpose

   Mammalian expression of late endosome marker Venus-rab7a

   Depositor

   Nevin Lambert

   Article

   Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

   Insert

   Venus-rab7a (Rab7 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 Orai2

   Plasmid

   #16369

   Depositor

   Anjana Rao

   Article

   Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

   Insert

   Orai2 (ORAI2 Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC1-oROS-HT-CaaX

   Plasmid

   #216417

   Purpose

   Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   oROS-HT-CaaX
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. ITPKA_3xmScarletI

   Plasmid

   #112960

   Purpose

   In vivo visualization of F-actin (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   ITPKA
   

   Tags

   3x mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-GFP(9-7)

   Plasmid

   #165145

   Purpose

   Expresses GFP(9-7) in mammalian cells

   Depositor

   Daichi Kamiyama

   Article

   Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

   Insert

   GFP(9-7)
   

   Expression

   Mammalian

   Available Since

   April 8, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pTFG005_Spike_Inducible_3xFlag

   Plasmid

   #191578

   Purpose

   Inducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance).

   Depositor

   Joshua Leonard

   Article

   Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

   Insert

   SARS-CoV-2 Spike Protein (S Synthetic)
   

   Use

   Lentiviral

   Tags

   3x FLAG

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET30b-5M SrtA with TEV site

   Plasmid

   #86962

   Purpose

   Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

   Depositor

   Ron Bose

   Article

   Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

   Insert

   S.aureus SrtA 5M
   

   Tags

   6x Histidine tag and TEV site

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T

   Promoter

   T7

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCKFB1

    Plasmid

    #31280

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

    Insert

    Clock (Clock Mouse)
    

    Tags

    Flag and His

    Expression

    Insect

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

11. SIRT3 Flag

    Plasmid

    #13814

    Purpose

    Mammalian expression of human SIRT3 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT3 (SIRT3 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX

    Plasmid

    #188994

    Purpose

    A plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    K57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-GID1

    Plasmid

    #37306

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GID1
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 19, 2012

    Availability

    Academic Institutions and Nonprofits only

14. HLA_A2402

    Plasmid

    #165010

    Purpose

    Expression of HLA-A*24:02 cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    HLA-A*24:02 (HLA-A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUW-tetO-hOKMS

    Plasmid

    #51543

    Purpose

    Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

    Depositor

    Tarjei Mikkelsen

    Article

    Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

    Insert

    hOKMS (MYC, KLF4, SOX2, POU5F1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-19b_MMLV-RT

    Plasmid

    #153312

    Purpose

    Bacterial expression of MMLV reverse transcriptase

    Depositor

    Drew Endy, Philippa Marrack

    Insert

    MMLV Reverse Transcriptase
    

    Tags

    10x His

    Expression

    Bacterial

    Available Since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pBABE GFP

    Plasmid

    #10668

    Purpose

    Empty backbone for retroviral gene expression. Select recipient cells using GFP.

    Depositor

    William Hahn

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQFmcs-2H12.D11-scarREF

    Plasmid

    #221184

    Purpose

    cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen2
    mScarlet-I
    

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    None (same as cdGreen2) and PQ5 (cumate-inducible)

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-NPC1(WT)-FLAG

    Plasmid

    #164972

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.CAG.Flex.Twitch2B.WPRE.SV40

    Plasmid

    #100039

    Purpose

    AAV expression of FLEXed Twitch2B driven by CAG promoter. Twitch 2B is a fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch2B
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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