179,960 results
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Plasmid#105539PurposeAAV expression of EGFP from hSyn promoterDepositorHas serviceAAV1InsertEGFP
UseAAVExpressionMammalianPromoterhSynAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR
Plasmid#104567PurposeMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activatorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
UseCRISPRTagsNLS-3xHA-VPRExpressionMammalianMutationD832APromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.Syn.GCaMP6s.WPRE.SV40
Plasmid#100843PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas serviceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-pro-Il1b-C-Flag
Plasmid#75131PurposeExpresses C-terminal flag-tagged pro-IL-1β in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertinterleukin 1 beta (Il1b Mouse)
Tags3XFLAGExpressionMammalianMutation(please see depositor comments below)PromoterCMVAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCI-neo Fluc EGFP
Plasmid#90170PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteinsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLuc EGFP
ExpressionMammalianAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCI-neo FlucDM EGFP
Plasmid#90172PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLuc EGFP
ExpressionMammalianMutationR188Q, R261Q in FLucAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pME-GAL4-ERT2-VP16
Plasmid#82588PurposeMiddle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAL4-ERT2-VP16
PromoterNoneAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-TA-ERN
Plasmid#80474PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonExpressionMammalianAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-smURFP-RBS-HO-1
Plasmid#80341PurposeExpresses smURFP and Heme Oxygenase-1 in bacterial cells.DepositorInsertssmURFP
HO-1
Tags6 Histidine TagExpressionBacterialPromoterpBADAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGWB502
Plasmid#74844PurposeGateway cloning compatible binary vector for expression of gene by CaMV35S promoter.DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYFP-RPB1aAmr
Plasmid#75284PurposeExpresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPOLR2A (POLR2A Human)
TagsEYFPExpressionMammalianMutationN792D alpha-amanitin–resistantPromoterCMVAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCIP-AMPKa1_WT
Plasmid#79010PurposeFor mammalian expression of WT AMPK alpha1.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAMPK alpha 1 (PRKAA1 Human)
ExpressionMammalianPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Flag::UbvG08 I44A, deltaGG
Plasmid#74939PurposeUbiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUbiquitin
TagsFlagExpressionMammalianMutationUbvG08 with I44A mutation and no terminal GlycinesPromoterCMVAvailable sinceJune 30, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCAG-SpCas9-GFP-U6-gRNA
Plasmid#79144PurposeAll-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSCDepositorInsertSpCas9
UseCRISPRTags2A-GFPExpressionMammalianPromoterCAGAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEBB Flag GCN5
Plasmid#74784Purposeexpress of GCN5 in mammalian cellsDepositorAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28b-BE1
Plasmid#73018PurposeExpresses BE1 with N-terminal His6 tag in E. ColiDepositorInsertBE1
TagsHis6 tagExpressionBacterialPromoterT7Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK294 (mAID-mCherry2-Bsr)
Plasmid#72832PurposeC-terminal tagging with mAID-mCherry2 using CRISPR/Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmAID-mCherry2-Bsr
UseCRISPRTagsmAID-mCherry2ExpressionMammalianAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ornithine transcarbamylase (OTC)
Plasmid#71877PurposeFor mitochondrial unfolded protein response researchDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOTC (Otc Rat)
TagsNoneExpressionMammalianPromoterCAGAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-PGK
Plasmid#72268PurposeExpress gene of interest under PGK (phosphoglycerate kinase I) promoterDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterPGKAvailable sinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pcdna nesprin HL
Plasmid#68128Purposenesprin headless controlDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnesprin-2 (Syne2 Mouse)
ExpressionMammalianMutationdeleted actin-binding domainPromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by