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pcDNA3-ICUE3-NLS

Plasmid

#138218

Purpose

Nuclear-targeted FRET-based cAMP indicator.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

ICUE3-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-LBR-mRFP1-polyA

Plasmid

#59749

Purpose

Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

LBR-mRFP1

Tags

mRFP1

Expression

Mammalian

Mutation

aa 1-249 of LBR

Promoter

CMV and T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pJWV25

Plasmid

#61045

Purpose

To construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via double crossover at bgaA

Depositor

Jan-Willem Veening

Article

Eberhardt et al Mol Microbiol. 2009 Oct;74(2):395-408. d

Type

Empty backbone

Use

Synthetic Biology

Tags

GFP+

Expression

Bacterial

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLD-puro-CcVA

Plasmid

#24588

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pWXLd-RIEP-CeNLuc

Plasmid

#135934

Purpose

Expresses the CeNLuc LumiFluor in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

CeNLuc LumiFluor

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSplit-CO-hDib1

Plasmid

#58788

Purpose

Express LucC-hDib1 fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDib1 (TXNL4A Human)

Tags

Flag and Luciferase (394-550aa)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 tdAPX W41F

Plasmid

#40863

Purpose

Tandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386).

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

tandem dimer pea APX W41F

Tags

Flag

Expression

Mammalian

Mutation

W41F mutation

Promoter

CMV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pFH2.104_PB-EPN24-T2A-eGFP-PGK-Blast

Plasmid

#205549

Purpose

Express EPN24-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCCl-MCS_miniCMV_TagBFP

Plasmid

#134986

Purpose

The plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Article

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

TagBFP

Use

Lentiviral

Promoter

minCMV

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARα

Plasmid

#119913

Purpose

Mammalian expression of the tAKARα sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARα

Expression

Mammalian

Available Since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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pET sfGFP(150TAG)

Plasmid

#133455

Purpose

expresses sfGFP with an unnatural amino acid at the 150th position in bacterial cells

Depositor

Yu-Hsuan Tsai

Article

Williams et al Amino Acids. 2021 Jan;53(1):89-96. doi:

Insert

sfGFP

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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TB:4xHRE_RE:MiniP

Plasmid

#178313

Purpose

4 copies of the HRE DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

4 copies of the heat shock DNA binding motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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eGFP L138 gRNA

Plasmid

#119133

Purpose

gRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

eGFP L138 gRNA

Use

CRISPR

Mutation

None

Promoter

U6

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-P2A-3xFLAG-hEEA1 HDR template

Plasmid

#214989

Purpose

Endogenously tag human EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (EEA1 Human)

Use

CRISPR

Tags

3xFLAG

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-QFGal4-SV40pA

Plasmid

#155126

Purpose

In vitro transcription of QFGal4 mRNA

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QFGal4

Use

Expression in zebrafish

Expression

Mammalian

Promoter

SP6

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJB38-NWMN2930

Plasmid

#84457

Purpose

pJB38 plasmid containing the S.aureus Newman genetic region between genes 29 and 30

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

Staphylococcus aureus NWMN genomic region NWMN29-30

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pWXLd-RIEP-GpNLuc

Plasmid

#135935

Purpose

Expresses the GpNLuc LumiFluor in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

GpNLuc LumiFluor

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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Orai1-myc E106Q MO70

Plasmid

#22754

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

Orai1 E106Q (ORAI1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

E106Q

Available Since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pKin1A

Plasmid

#31607

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

kinesin-1A (Kif5a Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-OCT4

Plasmid

#69537

Purpose

Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

5 concatenated gRNA transcriptional cassettes targeting OCT4

Use

CRISPR

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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