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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,785 results for: emb

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Showing: 5541 - 5560 of 11785 results

  1. KTD101(DE3)

    Bacterial strain
    #138651
    Purpose
    KTD101(DE3) is a trigger factor deficient strain, which may increase protein secretion from Escherichia coli, and is compatible with expression from the T7 promoter
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Bacterial resistance
    None
    Species
    Escherichia coli
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only

  2. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

    Plasmid
    #202553
    Purpose
    Nontargeting control vector with TagBFP2
    Depositor
    Frederic Meunier
    Publication
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Mutation
    Puromycin-resistance gene in the pLV hU6-sgRNA hU…
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202553-plasmid-map-sequence-id-404692

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  6. pAAH3

    Plasmid
    #41827
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.
    Depositor
    Melissa Moore
    Publication
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Yeast
    Promoter
    TEF
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41827-plasmid-map-sequence-id-69168

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  7. PT7-alphaHL

    Plasmid
    #227650
    Purpose
    pT7-driven expression of alpha-hemolysin, a pore-forming protein in lipid bilayers.
    Depositor
    Richard Murray
    Insert
    alpha-hemolysin
    Use
    Synthetic Biology
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    227650-plasmid-map-sequence-id-452398

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Showing: 5541 - 5560 of 11785 results