We narrowed to 65,766 results for: des.1

1. lentiCRISPR - Amplicon, BCR/ABL sgRNA 1

   Plasmid

   #70659

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-IP-EGFP-ULK1(1-828)

   Plasmid

   #38198

   Depositor

   Noboru Mizushima

   Article

   Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

   Insert

   a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 829-1051

   Promoter

   Available since

   Sept. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

3. p3XFlag-CMV10-Flag-mMcl-1 KR

   Plasmid

   #32979

   Depositor

   Joseph Opferman

   Article

   Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

   Insert

   Mcl-1 (Mcl1 Mouse)
   

   Use

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   All lys changed to Arg

   Promoter

   CMV

   Available since

   March 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. 813 pSG5L HA PTEN 1-274

   Plasmid

   #10740

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Contains PTEN amino acids 1-274.

   Promoter

   Available since

   Sept. 27, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pLKO human shRNA 1 c-MAF

   Plasmid

   #29432

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2009 May 13. ():.

   Insert

   c-MAF shRNA 1 (MAF Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)

   Plasmid

   #61939

   Purpose

   Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Inserts

   hUCH37 (ISF1) (UCHL5 Human)
   hNFRKB (1-156)
   

   Use

   Tags

   6x HIS

   Expression

   Bacterial

   Mutation

   Construct ends at residue 156

   Promoter

   T7

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

7. PCDNA3-N3FLAG-MYB-DBD(1-200)

   Plasmid

   #105610

   Purpose

   transient express MYB DBD fragment with 3*FLAG tag at N terminal in HEK 293T cells

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   DBD fragment of MYB (mouse), amino acids 1-200 (Myb Mouse)
   

   Use

   Tags

   3*FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. CA-0201 CMVTO-hIL6-rens.1

   Plasmid

   #231197

   Purpose

   CleaveIL-6

   Depositor

   Xiaojing Gao

   Article

   Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

   Insert

   hIL6-rens.1 (IL6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3puro-mGFP-FMR1iso1-N1(1-445)

   Plasmid

   #225464

   Purpose

   Express mEGFP-fusion protein of FMR1 fragment (1-445)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FMR1 (FMR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   N1 fragment (1-445)

   Promoter

   CMV

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3puro-mGFP-FMR1iso1-N2(1-491)

    Plasmid

    #225465

    Purpose

    Express mEGFP-fusion protein of FMR1 fragment (1-491)

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FMR1 (FMR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    N2 fragment (1-491)

    Promoter

    CMV

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. TL1 alphaL_DdCBE 1 (left, DddA11-C)

    Plasmid

    #221996

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alphaL-1 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: T1413I

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEM-601[insert(+1) TA-rich]

    Plasmid

    #221196

    Purpose

    601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

    Depositor

    Gregory Bowman

    Article

    Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

    Insert

    601[insert(+1)TA-rich]
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    601 has an additional mutation added 22 bp from d…

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSF1b-His8-TEV-PI31-1-151

    Plasmid

    #202593

    Purpose

    Bacterial expression of HsPI31-1-151

    Depositor

    Brenda Schulman

    Article

    Kraus et al EMBO Rep. 2023 Aug 3;24(8):e56399. doi:

    Insert

    PI31 (PSMF1 Human)
    

    Use

    Tags

    8xHis, TEV

    Expression

    Bacterial

    Mutation

    aa 1-151 only

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac1-His6-E3(1)-CUbo-TwinStrep

    Plasmid

    #212712

    Purpose

    Expression of His6-E3(1)-CUbo-TwinStrep in insect cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Tags

    6xHis and CUbo-TwinStrep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-FRT-TO-Myc-E3(1)-CUbo

    Plasmid

    #212799

    Purpose

    Constitutive/Doxycycline-inducible expression of Myc-E3(1)-CUbo in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Tags

    CUbo and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-Best1-nt.Txnip(C247S)(1-320aa)

    Plasmid

    #206352

    Purpose

    AAV plasmid expressing deltion and mutant TXNIP in retinal pigment epithelium

    Depositor

    Connie Cepko

    Article

    Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

    Insert

    Txnip (Txnip Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    (1-320aa), C247S

    Promoter

    human Best1

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-RedO-nt.Txnip(C247S)(1-301aa)

    Plasmid

    #206354

    Purpose

    AAV plasmid expressing deltion and mutant TXNIP in photoreceptors

    Depositor

    Connie Cepko

    Article

    Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

    Insert

    Txnip (Txnip Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    (1-301aa), C247S

    Promoter

    human red opsin

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX4T-1-RUNX3 aa 49-187

    Plasmid

    #203440

    Purpose

    Bacterial expression of RUNX5

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    RUNX3 (RUNX3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-THTAP-SPARTIN, residues 1-101

    Plasmid

    #180626

    Purpose

    Bacterial expression for SPARTIN MIT residues 1-101. Has N-terminal HIS-GST tag. Internal ID: WISP20-57.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPARTIN
    

    Use

    Tags

    HIS-GST

    Expression

    Bacterial

    Mutation

    Residues 1-101

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-THTAP-CALPAIN-7 1-165

    Plasmid

    #180610

    Purpose

    Bacterial expression vector for CALPAIN-7 residues 1-165. Has N-terminal cleavable HIS-GST tag.Internal ID: WISP21-129

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Tags

    HIS-GST

    Expression

    Bacterial

    Mutation

    Residues 1-65

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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