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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 24,746 results for: CHI

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Showing: 1351 - 1400 of 24746 results

  6. pEcRNAP6

    Plasmid
    #128940
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z
    Depositor
    Seth Darst
    Article
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    Tags
    His
    Expression
    Bacterial
    Mutation
    PreScission protease site (GE Healthcare; LEVLFQG…
    Promoter
    T7
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128940-plasmid-map-sequence-id-254597

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  17. pGL

    Plasmid
    #119952
    Purpose
    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102
    Inserts
    gpps
    limS
    Expression
    Bacterial
    Mutation
    N-terminal truncation of 56 amino acid residues (…
    Available Since
    Feb. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119952-plasmid-map-sequence-id-235949

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  30. pGBT-RP2.2

    Plasmid
    #189842
    Purpose
    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 2.
    Depositor
    Stephen Ekker
    Article
    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    GFP GM2 variant and mRFP
    Promoter
    beta Actin
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    189842-plasmid-map-sequence-id-373952

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  31. pGBT-RP2.3

    Plasmid
    #189843
    Purpose
    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 3.
    Depositor
    Stephen Ekker
    Article
    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    GFP GM2 variant and mRFP
    Promoter
    beta Actin
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    189843-plasmid-map-sequence-id-373951

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  36. pEVOL-pAzF[TAG]

    Plasmid
    #164579
    Purpose
    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons
    Depositor
    Ryan Mehl
    Article
    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2
    Inserts
    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    araBAD, glnS, and proK
    Available Since
    May 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    164579-plasmid-map-sequence-id-392918

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  46. pPICZc-HsACTG1*-thymosinB-8His

    Plasmid
    #111147
    Purpose
    Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.
    Depositor
    Mohan Balasubramanian
    Article
    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827
    Insert
    actin gamma 1 (ACTG1 Human)
    Use
    Pichia pastoris integration vector
    Tags
    Thymosin beta and a His-tag
    Expression
    Yeast
    Mutation
    codon-optimised for expression in Pichia pastoris
    Promoter
    AOX1
    Available Since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111147-plasmid-map-sequence-id-214068

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Showing: 1351 - 1400 of 24746 results