We narrowed to 6,134 results for: nls

1. GFP-polyQ74-NLS

   Plasmid

   #231892

   Purpose

   Forms nuclear HTT partial exon 1 Q74-NLS aggregates

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q74-NLS (HTT Human)
   

   Use

   Tags

   EGFP and SV40 NLS

   Expression

   Mammalian

   Mutation

   CAG repeat expansion

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-NLS-mCherry

   Plasmid

   #231890

   Purpose

   mCherry-tagged nuclear rupture marker

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   mCherry-SV40 NLS-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-polyQ23-NLS

   Plasmid

   #231891

   Purpose

   Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q23-NLS (HTT Human)
   

   Use

   Tags

   EGFP and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT3TS-NLS-TurboID

   Plasmid

   #163849

   Purpose

   In vitro transcription of nls-TurboID. Parton lab clone JST

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   TurboID
   

   Use

   In vitro transcription of rna

   Tags

   nls

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBbi_RP_3XNLS-MicroID2-FLAG

   Plasmid

   #188523

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   3XNLS-MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-EF1aL-nlsGFP-W

   Plasmid

   #126688

   Purpose

   Constitutive, ubiquitous, nuclear-localized GFP expression.

   Depositor

   Darrell Kotton

   Article

   Tang et al Nat. Phys. 18, 1371–1378 (2022).

   Insert

   nlsGFP
   

   Use

   Lentiviral

   Tags

   6xHIS and NLS

   Expression

   Mutation

   Promoter

   EF1aL

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTetOne-mCherry-cNLS

   Plasmid

   #138527

   Purpose

   Inducible expression of mCherry with nuclear localization signal

   Depositor

   Christopher Grigsby, Ana Teixeira

   Article

   Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

   Insert

   mCherry
   

   Use

   Tags

   3x SV40 cNLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3Gs

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. V5-BS2-NLS (Nucleoplasmin)

   Plasmid

   #220342

   Purpose

   Mammalian expression vector BS2 in nucleus

   Depositor

   Bryan Dickinson

   Article

   Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

   Insert

   BS2
   

   Use

   Tags

   NLS (Nucleus localization sequence) and V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCMV-PV-NLS-GFP

   Plasmid

   #17300

   Depositor

   Anton Bennett

   Article

   Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

   Insert

   PV-GFP-NLS (Pvalb Rat)
   

   Use

   Tags

   Myc and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-C1 EGFP-3XNLS

    Plasmid

    #58468

    Purpose

    Expresses a nuclear-targeted eGFP on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

    Insert

    3X NLS with 3X stop codons
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWT35S-Citrine-NLS

    Plasmid

    #182583

    Purpose

    Transient expression of Citrine-NLS in plant cell (Nucleus)

    Depositor

    Yutaka Kodama

    Article

    Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

    Insert

    NLS
    

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. NLS-SpCas9mSA-His

    Plasmid

    #182951

    Purpose

    Express SpCas9mSA in bacteria

    Depositor

    Wen Xue

    Article

    Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

    Insert

    NLS-SpCas9mSA-His
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF-APEX2-NLS

    Plasmid

    #140281

    Purpose

    Expression of APEX2-NLS

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    APEX2-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIBN(deltaNLS)-pmGFP

    Plasmid

    #26867

    Purpose

    Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    NLS sequences mutated

    Promoter

    CMV

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB_SBE4:mScarlet-I-NLS

    Plasmid

    #154961

    Purpose

    encodes transcriptional reporter of SMAD2/3

    Depositor

    Ewa Bielczyk-Maczynska, Joshua Knowles

    Article

    Taylor et al Sci Rep. 2020 Aug 21;10(1):14084. doi: 1

    Insert

    mScarlet
    

    Use

    Piggybac

    Tags

    NLS-PEST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. T2A 3xNLS GFP

    Plasmid

    #227721

    Purpose

    Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

    Depositor

    Oliver Hobert, Haosheng Sun

    Article

    Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

    Insert

    T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-AKAR4-NLS

    Plasmid

    #138217

    Purpose

    Nuclear-targeted FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    AKAR4-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRRL.EF1a.HA.NLS.Sce(opt).T2A.BFP

    Plasmid

    #32628

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Sce
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGWT35S-mCherry-NLS

    Plasmid

    #194049

    Purpose

    Transient expression of mCherry-NLS in plant cell (Nucleus)

    Depositor

    Yutaka Kodama

    Article

    Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

    Insert

    mCherry
    

    Use

    Tags

    SV40 (Nuclear localization signal)

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT3TS-RfxCas13d-NLS-HA

    Plasmid

    #141321

    Purpose

    Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos, Ariel Bazzini

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d (NLS-RfxCas13d-NLS)
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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