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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,684 results for: nts

Showing: 541 - 560 of 14684 results

  1. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70703-plasmid-map-sequence-id-127765

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  3. MSP2253

    Plasmid
    #70704
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70704-plasmid-map-sequence-id-127764

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  4. MSP2279

    Plasmid
    #70706
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A and H557A in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70706-plasmid-map-sequence-id-127768

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  5. MSP2372

    Plasmid
    #73196
    Purpose
    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, and Q926A in SpCas9
    Promoter
    CMV
    Available Since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73196-plasmid-map-sequence-id-134659

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  9. huCofilin S3E SSR

    Plasmid
    #245940
    Purpose
    Expresses human cofilin with mutation S3E that behaves like inactive phosphocofilin but can be dominant negative against phosphatase
    Depositor
    James Bamburg
    Article
    Agnew et al J Biol Chem. 1995 Jul 21;270(29):17582-7
    Insert
    cofilin 1 (cfl1) (CFL1 Human)
    Expression
    Mammalian
    Mutation
    S3E; silent mutations (NTs 67 to 75) in which TCT…
    Promoter
    CMV
    Available Since
    Oct. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    245940-plasmid-map-sequence-id-490033

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  18. XE57 Delta N-cadherin

    Plasmid
    #17025
    Depositor
    Randall Moon
    Insert
    dominant N-cadherin
    Use
    Xenopus expression
    Mutation
    nt 90-2004 deleted
    Available Since
    April 15, 2008
    Availability
    Academic Institutions and Nonprofits only
    17025-plasmid-map-sequence-id-337144

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  19. pAGNT-Sema3A

    Plasmid
    #16428
    Depositor
    Jonathan Raper
    Insert
    Sema3A (SEMA3A Chicken)
    Tags
    His and Myc
    Available Since
    Dec. 14, 2007
    Availability
    Academic Institutions and Nonprofits only

  20. NK691

    Plasmid
    #120850
    Purpose
    Reporter gene expression in S. rosetta
    Depositor
    Nicole King
    Insert
    Jumblelw1-mWasabi
    Use
    Choanoflagellate expression
    Mutation
    Jumblelw1 allele: T to C at nt 1109 causes L305P …
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120850-plasmid-map-sequence-id-233701

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Showing: 541 - 560 of 14684 results