We narrowed to 10,022 results for: transfer
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Plasmid#207466PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT)DepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only
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pHC01
Plasmid#106900PurposeRatiometric gas biosensor plasmid for 3-oxo-C12-HSL sensingDepositorInsertsLasR
Methyl Halide Transferase
Ethylene Forming Enzyme
Red Fluorescent Protein
ExpressionBacterialAvailable SinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pECFP_C1_NSD2 FL
Plasmid#221382PurposeExpresses NSD2 in mammalian cellsDepositorInsertHistone-lysine N-methyltransferase NSD2 (NSD2 Human)
ExpressionMammalianMutationL237R mutation in ECFP (Please see depositor comm…PromoterCMVAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPAT-2XStrep
Plasmid#125620Purposeexpresses human PPAT protein with a C-terminal 2XStrep affinity tagDepositorAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_NSD2
Plasmid#221376PurposeExpresses NSD2 in bacterial cellsDepositorAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHPHS800
Plasmid#228240PurposeBxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombinationDepositorInsertCas9
TagsHygromycin phosphotransferaseExpressionMammalianPromoternoneAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-MjDim1
Plasmid#189936PurposeExpression of Mjdim1, a methyltransferase used in m6A-SAC-seq.DepositorInsertrsmA (MJ_RS00455 Methanocaldococcus jannaschii)
Tags6x His tag, FLAG tag, SUMO tagExpressionBacterialPromoterT7 promoterAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
dSpyCas9-p300-P2A-CASANOVA
Plasmid#113129PurposeCo-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVADepositorInsertdCas9-p300-P2A-CASANOVA
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRedCmOcr
Plasmid#176226PurposeA helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.DepositorInsertLambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyExpressionBacterialPromoterPrhaB and PLAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLgw RFC1-V5-EcoDam
Plasmid#59205PurposeMammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloningDepositorTypeEmpty backboneUseLentiviral; DamidTagsDam (DNA adenine methyltransferase) and V5ExpressionMammalianPromoterHeat Shock Minimal PromoterAvailable SinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET19-MTdTwt
Plasmid#207460PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT)DepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h1
Plasmid#86689PurposeFor transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'DepositorAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBM019
Plasmid#128179PurposeConstruct for generating stable Cas9-expressing Toxoplasma gondiiDepositorInsertssgRNA#1
SpCas9
Chloramphenicol acetyltransferase
UseCRISPR; Toxoplasma gondii expressionTags3X FLAG and Nuclear Localization SignalPromoterTUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast coSHMT2
Plasmid#106299PurposeExpresses SHMT2DepositorInsertserine hydroxymethyltransferase 2 (SHMT2 Human)
UseRetroviralExpressionMammalianMutationCodon optimizedAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
TfR-mCh-DHFR
Plasmid#173013PurposeFor light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.DepositorInsertTfR-mCh-DHFR
TagsDHFR and mCherryExpressionMammalianPromoterCMVAvailable SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
LLP783_SpyCatcher-DNMT3A-CO
Plasmid#211776PurposeSpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertTraptavudin-DNMT3A
Tags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cerulean-GalT
Plasmid#11930DepositorInsertGalT (GALT Human)
ExpressionMammalianMutationAmino acids 1-60 of the human galactosyltransfera…Available SinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRosa26-TG
Plasmid#40026DepositorInsertsT (ATG)
beta-globin intron
Neo
GFP
diphteria toxin A
ExpressionMammalianMutationdeleted nucleotides 1-274 and insertion of FRT, …PromoterCAG (chicken beta globin promoter and CMV enhance…Available SinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-MQ1(S317A)-EGFP
Plasmid#89636PurposeTargeted CpG methylationDepositorInsertsite-specific DNA-methyltransferase SssI
UseCRISPRTags3*FLAG and 6*HisExpressionMammalianMutationS317A, AGC-->GCCPromoterCMVAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-Mettl4
Plasmid#86665PurposeExpresses murine Mettl4 containing N-terminal 3XFlag tagDepositorAvailable SinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iHygro-L3 #BGV206
Plasmid#48985PurposeSupplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInserthygromycin phosphotransferase
UseGateway cloning vectorTagsIRES (weak)PromoternaAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
LLP784_SnoopCatcher-DNMT3A-CO
Plasmid#211777PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertSpy-EZH2
Tags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINDgw RFA-V5-EcoDam
Plasmid#59204PurposeMammalian DamID vector for fusing gene of interest with Dam-V5 using Gateway cloningDepositorTypeEmpty backboneUseDamidTagsDam (DNA adenine methyltransferase) and V5ExpressionMammalianPromoterHeat Shock Minimal PromoterAvailable SinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSKA397
Plasmid#80380Purposemini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.DepositorInsertscat
metE
UseSynthetic Biology; Mini-tn7 delivery vectorExpressionBacterialPromotercat promoter and cpcG2Δ59Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron(Neo)-G(LNL)
Plasmid#36908DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Neo
ExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
LLP789_αGCN4-DNMT3A-CO
Plasmid#211783PurposeSunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertaGCN4-DNMT3A
Tags3xTy1ExpressionMammalianMutationLast 300 bp codon optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iPuro-L3 #BGV205
Plasmid#48987PurposeSupplies the selection marker (puromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertpuromycin resistance
UseGateway cloning vectorTagsIRES (weak)PromoterNAAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron(Neo)-T(FLLFLNL)
Plasmid#40020DepositorInsertsGFP
tdT-3Myc
beta-globin intron
Neo
Tags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta globin promoter and CMV enhance…Available SinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBT250_(pCA-G-intron(Neo)-tTA2)
Plasmid#36876DepositorInsertsGFP
tTA2
beta-globin intron
Neo
ExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLTR PKC zeta
Plasmid#8421DepositorInsertMouse Protein Kinase C zeta (Prkcz Mouse)
ExpressionMammalianAvailable SinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only