We narrowed to 7,663 results for: ski

1. AT5G59650 O4_pECIA2

   Plasmid

   #114964

   Purpose

   Bait vector AT5G59650 O4_pECIA2 should be used with prey vector AT5G59650 O4_pECIA14.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT5G59650 (AT5G59650 Mustard Weed)
   

   Tags

   Fc, V5 and hexahistidine tags

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIBN-LacI

   Plasmid

   #103814

   Purpose

   BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-LacI (CIB1, lacI Mustard Weed, E. coli)
   

   Expression

   Mammalian

   Mutation

   LacI: C19T (silent, L7L), T264C (silent, A88A), C…

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_RBM45_WT

   Plasmid

   #82892

   Purpose

   Gateway Donor vector containing RBM45, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   RBM45 (RBM45 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.S72A

   Plasmid

   #106191

   Purpose

   Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-Venus-iGluSnFR.S72A
   

   Use

   AAV

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

   Promoter

   CAG

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

   Plasmid

   #157693

   Purpose

   mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pFUGW-shRIIα-RIIαWT-IRES-EGFP

   Plasmid

   #183456

   Purpose

   Replacement of endogenous rat PKA-RIIα subunits with wild-type RIIα

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA and Prkar2a (Prkar2a Rat)
   

   Use

   Lentiviral

   Mutation

   shRNA-resistant mutations: C135>G, G138>A, …

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184V

   Plasmid

   #106184

   Purpose

   Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-Venus-iGluSnFR.A184V
   

   Use

   AAV

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

   Promoter

   hSynapsin

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR1A-PIK3CA E545K

   Plasmid

   #180032

   Purpose

   Entry vector of PIK3CA E545K for gateway cloning.

   Depositor

   Xin Chen

   Article

   Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

   Insert

   PIK3CA E545K (PIK3CA Human)
   

   Use

   Entry vector

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   E545K

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Esrrb gRNA

   Plasmid

   #128841

   Purpose

   gRNA for targeting mouse Esrrb locus using CRISPR-cas technique

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

   Insert

   Esrrb gRNA (Esrrb Mouse)
   

   Use

   CRISPR

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-myc-CXCR4-W161Y

    Plasmid

    #98951

    Purpose

    Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161Y

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    W161Y: enhanced CXCL12 binding and signaling

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-myc-CXCR4-W161H

    Plasmid

    #98952

    Purpose

    Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161H

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    W161H: enhanced CXCL12 binding and signaling

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. W118-1_hRSK3(RPS6KA2)

    Plasmid

    #180384

    Purpose

    lentiviral transduction of human RSK3 (RPS6KA2) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    RPS6KA2 (RPS6KA2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBOB-N-3HA-GK_v1

    Plasmid

    #134275

    Purpose

    Lentivector encoding 3XHA-tagged GK (isoform 1)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk (Gk Mouse)
    

    Use

    Lentiviral

    Tags

    3X HA

    Expression

    Mammalian

    Mutation

    524 amino acids, isoform 1

    Promoter

    CMV

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIBN-EYFP-hNCL

    Plasmid

    #103806

    Purpose

    BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hNCL (CIB1, NCL Human, Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. integrin alpha9 / alpha5 pcDNA1 neo

    Plasmid

    #13588

    Depositor

    Dean Sheppard

    Article

    Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

    Insert

    integrin alpha 9 / alpha 5 (ITGA9 Human)
    

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX Jak3-SH2

    Plasmid

    #46449

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Jak3 (JAK3 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 366-494

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-myc-CXCR4-H79Q

    Plasmid

    #98953

    Purpose

    Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant H79Q

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    H79Q: enhanced CXCL12 binding and signaling

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEE

    Plasmid

    #159746

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX Socs2-SH2

    Plasmid

    #46505

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Socs2 (SOCS2 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 28-162

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG-RFP-UCR1C

    Plasmid

    #67582

    Purpose

    Expresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cells

    Depositor

    Graeme Carnegie

    Article

    Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

    Insert

    C-terminus of upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)
    

    Tags

    FLAG and TagRFP

    Expression

    Mammalian

    Mutation

    Amino acid residues 80-136 of PDE4D3

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only