167,318 results

1. pSBFP2-C1

   Plasmid

   #22880

   Depositor

   Dorus Gadella

   Article

   Kremers et al Biochemistry. 2007 Mar 27. 46(12):3775-8

   Insert

   SBFP2
   

   Expression

   Mammalian

   Available Since

   Feb. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-sgRNA neo

   Plasmid

   #104992

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX_305

   Plasmid

   #41390

   Purpose

   Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag

   Depositor

   David Root

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. NbV5-LgBit

   Plasmid

   #201475

   Purpose

   NanoBit detection of V5-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbV5-LgBit
   

   Tags

   LgBit

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. VPS13A^Halo

   Plasmid

   #118759

   Purpose

   VPS13A internally tagged with Halo at residue 1372.

   Depositor

   Pietro De Camilli

   Article

   Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

   Insert

   VPS13A (VPS13A Human)
   

   Tags

   Halo tag inserted at position 1372

   Expression

   Mammalian

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-PEmaxRNaseHtrunc

   Plasmid

   #207858

   Purpose

   Mammalian expression of PEmaxDRNaseH prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PEmaxDRNaseH
   

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   M-MLVRTDRNAseHrt

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. piggyBAC-BRD4-Flag

   Plasmid

   #214432

   Purpose

   A piggyBAC based plasmid for the stable expression of BRD4 with a flag tag.

   Depositor

   Shaun Cowley

   Insert

   Brd4 bromodomain containing 4 (Brd4 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3XMEF2-luc

   Plasmid

   #32967

   Purpose

   Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter

   Depositor

   Ron Prywes

   Insert

   MEF2 binding sites (MEF2A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Promoter

   MEF2

   Available Since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-CamKIIa-jGCaMP8s-WPRE (AAV5)

   Viral Prep

   #176752-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from AAV-CamKIIa-jGCaMP8s-WPRE (#176752). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8s-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   CamKIIalpha

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

10. Lys-eMagA-EGFP

    Plasmid

    #162246

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    p18-eMagA-EGFP
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV5)

    Viral Prep

    #162381-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (#162381). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    CAG

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

12. MmXYRS-4xM15

    Plasmid

    #155343

    Purpose

    Plasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.

    Depositor

    Irene Coin

    Article

    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

    Insert

    Humanized MmXYRS/tRNAM15
    

    Expression

    Mammalian

    Promoter

    CMV/U6

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-Traditional CAR (anti-CD19)

    Plasmid

    #192115

    Purpose

    Lentiviral delivery of Traditional CAR(anti-CD19)

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pHR-pEF1a-Traditional CAR (anti-CD19scFV_CD28_41BB_CD3z)
    

    Use

    Lentiviral

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4-FLAG-Jhdm2a

    Plasmid

    #38136

    Depositor

    Toshinobu Nakamura, Toru Nakano

    Article

    Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

    Insert

    Jhdm2a (Kdm3a Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    protein starts at aa115

    Promoter

    CMV

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. Mouse Chromatin Regulator Library

    Pooled Library

    #200011

    Purpose

    CRISPR KO library targeting a comprehensive set of genes with annotated function as chromatin regulators.

    Depositor

    Bradley Bernstein, Robert Manguso, Gabriel Griffin

    Article

    Griffin et al Nature. 2021 Jul;595(7866):309-314. doi:

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

16. pET His6 MBP TEV LIC cloning vector (1M)

    Plasmid

    #29656

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCT5-bac1.8

    Plasmid

    #119871

    Purpose

    Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

    Depositor

    Claudia Schmidt-dannert

    Article

    Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

    Inserts

    CymR'
    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    SNP in 592 bp

    Promoter

    PserA promoter and Pveg promoter with CuO sequence

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX307-CRBN-3F-BirA

    Plasmid

    #220146

    Purpose

    Expresses CRBN-BirA for CRBN substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    Cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    3F-BirA

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-hRad51

    Plasmid

    #125570

    Purpose

    Expresses the Rad51 protein in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51 (RAD51 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-cmyc

    Plasmid

    #16011

    Purpose

    mammalian expression of human cmyc

    Depositor

    Wafik El-Deiry

    Article

    Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

    Insert

    cmyc (MYC Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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