161,697 results

1. AAV-mDlx-jGCaMP8m-WPRE (AAV9)

   Viral Prep

   #176754-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from AAV-mDlx-jGCaMP8m-WPRE (#176754). In addition to the viral particles, you will also receive purified AAV-mDlx-jGCaMP8m-WPRE plasmid DNA. mDlx enhancer-driven expression of calcium sensor GCaMP8m in GABA-ergic interneurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   mDlx

   Tags

   None

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

2. phage UbiC NLS HA stdMCP Halo

   Plasmid

   #104098

   Purpose

   Lentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   stdMCP-Halo
   

   Use

   Lentiviral

   Tags

   C-terminal Halo-tag, N-terminal NLS, and Two syno…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-opCas12a-6xNLS

   Plasmid

   #209022

   Purpose

   Lentiviral vector expressing human codon-optimized OpCas12a with 6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   opCas12a-6xNLS-Flag-Myc-NeoR
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and MYC

   Expression

   Mammalian

   Mutation

   E174R/S542R

   Promoter

   EF-1a

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-ZDHHC17

   Plasmid

   #231665

   Purpose

   Expresses human ZDHHC17protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   ZDHHC17 (ZDHHC17 Human)
   

   Use

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS

   Plasmid

   #89508

   Purpose

   Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2017 Feb 27. doi: 10.103

   Insert

   rAPOBEC1-nCas9 (D10A)-UGI-NLS
   

   Use

   Tags

   UGI and rAPOBEC-XTEN

   Expression

   Bacterial

   Mutation

   D10A mutation of Cas9

   Promoter

   T7

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWS3799-gRNA-Csy4-template

   Plasmid

   #182827

   Purpose

   SpCas9 gRNA-Csy4 template

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Insert

   SpCas9 gRNA scaffold + Csy4 sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. MZB211

   Plasmid

   #228494

   Purpose

   10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)

   Depositor

   Richard Baker

   Insert

   pol
   

   Use

   Tags

   10xHis-SUMO(Smt3)

   Expression

   Bacterial

   Mutation

   A408S

   Promoter

   T7 Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hsyn-DIO-GCaMP6s

   Plasmid

   #184284

   Purpose

   Expresses GCaMP6s in genetically defined neurons expressing Cre recombinase

   Depositor

   William Wisden

   Article

   Yu et al Nat Neurosci. 2019 Jan;22(1):106-119. do

   Insert

   CCaMP6s
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   human synaptophysin

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223-NTRK1

   Plasmid

   #23891

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   NTRK1 (NTRK1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   V263L relative to NM_001012331

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PE7-SB2

    Plasmid

    #228868

    Purpose

    PE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequence

    Depositor

    Sangsu Bae

    Insert

    PE7-2A-MLH1-SB-NLS-2A-MLH1-SB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Str-Golgin84_VSVG-SBP-EGFP

    Plasmid

    #65305

    Purpose

    synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Golgin-84 (GOLGA5 Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORANGE Dlg4-GFP KI

    Plasmid

    #131477

    Purpose

    Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Dlg4, Dlg4 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-HDR-mEGFP-Actin

    Plasmid

    #119870

    Purpose

    AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse Beta Actin

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    beta Actin (Actb Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. GW1-pHRed

    Plasmid

    #31473

    Purpose

    genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

    Depositor

    Gary Yellen

    Article

    Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

    Insert

    pHRed
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2FA CO Tol2 TPase

    Plasmid

    #133032

    Purpose

    Expression vector for production of codon optimized Tol2 transposase

    Depositor

    Charles Nathan Hancock

    Article

    Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

    Insert

    Codon optimized Tol2 Transposase
    

    Use

    In vitro expression

    Tags

    Expression

    Mutation

    Codon Optimized

    Promoter

    Available since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSF-p15A-trc- YFP

    Plasmid

    #186569

    Purpose

    p15A ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

    Depositor

    Teresa de Diego Puente

    Article

    Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

    Insert

    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_AncBE4max

    Plasmid

    #112094

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    AncBE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

    Plasmid

    #180475

    Purpose

    Inducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Insert

    Zebrafish ubb promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-mPGK-eGFP (beta-globin intron)

    Plasmid

    #162513

    Purpose

    AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intron

    Depositor

    Bart Nieuwenhuis

    Article

    Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mPGK

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. ZCCHC6

    Plasmid

    #156140

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    ZCCHC6 (TUT7 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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