We narrowed to 41,611 results for: KAN

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pGL3-EF1a-i53

Plasmid

#216243

Purpose

Express i53 in mammalian cells as a HDR enhancer

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

i53

Tags

Flag

Expression

Mammalian

Promoter

EF1a

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-N-TEV-Twin-Strep

Plasmid

#202526

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

TEV cleavable site and Twin-strep-tag

Expression

Mammalian

Promoter

CAG

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB442

Plasmid

#74828

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-G-GECO1.2

Plasmid

#32446

Purpose

Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.2

Expression

Mammalian

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pBGSA FPR1-EGFP

Plasmid

#62604

Purpose

Constitutive expression of FPR1-EGFP in mammalian cells

Depositor

Heini Miettinen

Article

Gripentrog et al J Immunol. 2003 Sep 15;171(6):3187-93.

Insert

FPR1 (FPR1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

SRalpha

Available Since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pAGM55273

Plasmid

#153211

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP1339

Plasmid

#119271

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1337

Plasmid

#119270

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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TET1cd-dCas9-p300 (D18)

Plasmid

#239489

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-TET1cd-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-p300

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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human STIM1 YFP

Plasmid

#19754

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

YFP

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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MBP-C5E-6XTUBE

Plasmid

#217628

Purpose

Bacterial expression of MBP tagged 6XTUBE

Depositor

Ethan Lee

Article

Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

Insert

6XTUBE

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB435

Plasmid

#74825

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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T7 promoter + terminators reporter plasmid

Plasmid

#156456

Purpose

A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Inserts

NeoR/KanR
TcR

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

Em7 and tet

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB414

Plasmid

#74808

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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PB_TetON_mCherry_P2AT2A_GFP-nb

Plasmid

#238239

Purpose

For integration of mCherry_P2AT2A_GFP-nb

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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Reckleen_3plus_sgRNA_ptet_pos4

Plasmid

#233459

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 4, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 4, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5

Plasmid

#233460

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGEM-myr-tdTomato-SV40pA-FKF

Plasmid

#119867

Purpose

Donor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

Insert

myr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Tags

2x myristoylation

Expression

Bacterial

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA Ago2 corrected

Plasmid

#10822

Depositor

Thomas Tuschl

Article

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

Argonaute 2 (AGO2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

3 silent mutations in orf

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-E2Crimson

Plasmid

#153425

Purpose

Retroviral (MSCV) expression of E2-Crimson fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

E2-Crimson

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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