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pGST2-Ub CPS (8-16)

Plasmid

#17631

Depositor

James Hurley

Article

Lee et al Nat Struct Mol Biol. 2006 Mar . 13(3):26

Insert

Ub CPS (8-16) (RPL40A Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
7861 pRS305-pUG72-HSC82out

Plasmid

#10899

Depositor

Susan Lindquist

Article

Cowen et al Science. 2005 Sep 30. 309(5744):2185-9.

Insert

HSC82 URA3 (HSC82 Budding Yeast)

Use

Cre/Lox

Expression

Yeast

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only

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pSKB3.TM1468

Plasmid

#11431

Depositor

Sung-Hou Kim

Article

Schulze-Gahmen et al Proteins. 2003 Mar 1. 50(4):526-30.

Insert

fatty acid binding protein

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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RMC sup35 promotor integr

Plasmid

#1175

Depositor

Susan Lindquist

Article

Sondheimer et al Mol Cell 2000 Jan;5(1):163-72.

Insert

Rnq1-Sup35 fusion (SUP35, RNQ1 Budding Yeast)

Expression

Yeast

Available Since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
pJW1668

Plasmid

#112042

Purpose

CEN/ARS plasmid for GAL-inducible FET3-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

FET3

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only

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pJW1669

Plasmid

#112043

Purpose

CEN/ARS plasmid for GAL-inducible NSG2-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

NSG2

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only

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pJW1672

Plasmid

#112046

Purpose

CEN/ARS plasmid for GAL-inducible PST1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

PST1

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only

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pJW1674

Plasmid

#112048

Purpose

CEN/ARS plasmid for GAL-inducible SWP1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

SWP1

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) A259C

Plasmid

#111329

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

branch site A259C

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) C256A

Plasmid

#111331

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

branch site C256A

Availability

Academic Institutions and Nonprofits only

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pMXs-3XHA-EGFP-OMP25

Plasmid

#83356

Purpose

For tagging mitochondria with HA epitopes (HA-MITO construct)

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XHA-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-N-mCherry

Plasmid

#123215

Purpose

N-terminal mCherry tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway destination

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-MTM1

Plasmid

#51614

Purpose

Recruitable PtdIns3P/PtdIns(3,5)P2 phosphatase

Depositor

Tamas Balla

Article

Hammond et al J Cell Biol. 2014 Apr 7.

Inserts

FKBP
MTM1 (MTM1 1812, Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pRK-flag-GFP11

Plasmid

#78590

Purpose

Expression flag-GFP 11 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-GFP 11

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pLS-mP

Plasmid

#81225

Purpose

Lentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.

Depositor

Nadav Ahituv

Article

Inoue et al Genome Res. 2016 Nov 9. pii: gr.212092.1

Type

Empty backbone

Use

Lentiviral

Promoter

Derived from pGL4.23

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-3XMyc-EGFP-OMP25

Plasmid

#83355

Purpose

For tagging mitochondria with Myc epitopes (Control-MITO construct)

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XMyc-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB327

Plasmid

#113671

Purpose

expresses Ulp1_R3 in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R3

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pYES2-Ub-M-GFP

Plasmid

#11952

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-M-GFP

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Methionine …

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pET24b-APEX2

Plasmid

#111702

Purpose

For IPTG-inducible expression of APEX2 in E. coli

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pJSK659

Plasmid

#205128

Purpose

Expression of GFP-SnxA in mammalian cells

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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