We narrowed to 17,323 results for: Por

1. pStr-KDEL_EPV-SBP-DsRed2-ER-5

   Plasmid

   #170676

   Purpose

   Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Inserts

   EPV-SBP-DsRed2-ER-5
   streptavidin-KDEL
   

   Expression

   Mammalian

   Promoter

   IRES and T7

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPPC017.109

   Plasmid

   #171145

   Purpose

   Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   J109 scRNA
   J1-BBa_J23117-mRFP
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119 and J1-BBa_J23117

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTrc99-Rat FABP2

   Plasmid

   #13578

   Depositor

   Martin Scanlon

   Article

   Velkov et al J Biol Chem. 2005 May 6. 280(18):17769-7

   Insert

   intestinal fatty acid binding protein (Fabp2 Rat)
   

   Expression

   Bacterial

   Available Since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pLenti-FNLSHiFi-P2A-Puro

   Plasmid

   #136902

   Purpose

   Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSHiFi
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Mutation

   R691A

   Promoter

   EF1s

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBXPHM3-PCFT_NB

   Plasmid

   #165415

   Purpose

   Expression in E.coli of N terminally MBP tagged anti-GgPCFT nano body

   Depositor

   Simon Newstead

   Article

   Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

   Insert

   GgPCFT_NB (SLC46A1 Llama, Chicken)
   

   Tags

   His10-MBP-3C protease site

   Expression

   Bacterial

   Promoter

   arabinose

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB194

   Plasmid

   #61154

   Purpose

   Expression of KIN28 under GAL1 promoter in yeast

   Depositor

   Charles Brenner

   Article

   Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

   Insert

   TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   Gal1

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pAL725

   Plasmid

   #119862

   Purpose

   precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C

   Depositor

   Linda Randall

   Article

   Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

   Insert

   D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
   

   Tags

   none

   Expression

   Bacterial

   Mutation

   Leucine 244 to Cysteine, Aspartate 287 to Cysteine

   Promoter

   T5

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLD-hygro-EnVM

   Plasmid

   #24590

   Depositor

   Jason Moffat

   Article

   Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

   Insert

   Gateway(TM) cassette
   

   Use

   Lentiviral

   Tags

   VM

   Expression

   Mammalian

   Available Since

   July 7, 2010

   Availability

   Academic Institutions and Nonprofits only

9. PA-DreN

   Plasmid

   #169707

   Purpose

   The optimized DreN246-nMag

   Depositor

   Dali Li

   Article

   Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

   Insert

   Dre246-nMag
   

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PA-DreC

    Plasmid

    #169705

    Purpose

    The optimized pMag-Dre247

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    pMag-Dre247
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSELt_S7.A

    Plasmid

    #125835

    Purpose

    Negative selection plasmid for evolution of an amber stop codon suppression tRNA

    Depositor

    Andrew Ellington

    Article

    Maranhao et al ACS Synth Biol. 2017 Jan 20;6(1):108-119

    Insert

    ACP-2xAmb-pheS
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pr.trpL*

    Available Since

    May 15, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET20b-GpNLuc-6xHis

    Plasmid

    #135932

    Purpose

    Expresses the GpNLuc LumiFluor in bacterial cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTK_Citrine_Nanotag_5_neg_Control

    Plasmid

    #130558

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    negative oligo sequence
    

    Use

    Enhancer cloning nanotag reporter vector

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH_ss-SBP-EGFP

    Plasmid

    #172358

    Purpose

    Reporter to monitoring a streptavidin-binding peptide trafficking

    Depositor

    Guido Kroemer

    Article

    Zhao et al Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.

    Insert

    ER importing signal sequence
    

    Use

    Lentiviral

    Tags

    SBP-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFH2.97_PB-EPN1-MCP-T2A-eGFP-PGK-Blast

    Plasmid

    #205542

    Purpose

    Express EPN1-MCP-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN1-MCP
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid

    #69547

    Purpose

    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Inserts

    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    

    Use

    Mouse Targeting

    Tags

    2x nuclear localization signal and PVT1-2A "…

    Mutation

    Silent V205V mutation in myogenin gene to prevent…

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFH2.102_PB-EPN11-MCP_I-T2A-eGFP-PGK-Blast

    Plasmid

    #205547

    Purpose

    Express EPN11-MCP-I-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN11-MCP-I
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFH2.103_PB-EPN11-MCP_p6-T2A-eGFP-PGK-Blast

    Plasmid

    #205548

    Purpose

    Express EPN11-MCP-p6-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN11-MCP-p6
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLD-puro-PGKnVA

    Plasmid

    #24589

    Depositor

    Jason Moffat

    Article

    Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

    Insert

    Gateway(TM) cassette
    

    Use

    Lentiviral

    Tags

    VA

    Expression

    Mammalian

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

20. A3F-Cas9n-UGI

    Plasmid

    #119138

    Purpose

    Base editor made from APOBEC3F

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3F-Cas9 nickase-UGI
    

    Use

    CRISPR

    Mutation

    None

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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