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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 29,873 results for: des.2

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Showing: 5621 - 5640 of 29873 results

  1. pADH147

    Plasmid
    #90991
    Purpose
    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    gRNA conserved, FRT, DS_HIS1
    Use
    CRISPR
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90991-plasmid-map-sequence-id-180524

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  3. pGBW-m4046955

    Plasmid
    #145616
    Purpose
    Bacterial Expression plasmid for RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    RNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145616-plasmid-map-sequence-id-307396

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  9. pCAGGS-Twin-strep-TEV-DGKδ2

    Plasmid
    #223720
    Purpose
    Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    DGKD (DGKD Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted start codon
    Promoter
    CAG and chicken β-actin promoter
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    223720-plasmid-map-sequence-id-457431

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  12. pmCellFree_KA1_SARS-COV-2_M

    Plasmid
    #169400
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus M with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _M (M SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169400-plasmid-map-sequence-id-336863

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  13. pmCellFree_KA1_SARS-COV-2_E

    Plasmid
    #169399
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus E with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _E (E SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169399-plasmid-map-sequence-id-335422

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  14. pmCellFree_KA1_SARS-COV-2_N

    Plasmid
    #169401
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus N with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _N (N SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169401-plasmid-map-sequence-id-336866

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  15. pmCellFree_KA1_SARS-COV-2_S

    Plasmid
    #169402
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus S with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _S (S SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    169402-plasmid-map-sequence-id-336167

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  16. pmCellFree_KA3_SARS-COV-2_RBD

    Plasmid
    #169405
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 RBD domain of S protein with C-term mCherry
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _RBD (S SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    mCherry
    Promoter
    T7- CMV/CAG
    Available Since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169405-plasmid-map-sequence-id-336878

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  17. pmCellFree_KA1_SARS-COV-2_ORF10

    Plasmid
    #169398
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF10 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF10 (ORF10 SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169398-plasmid-map-sequence-id-336872

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  18. pmCellFree_KA1_SARS-COV-2_ORF8

    Plasmid
    #169395
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF8 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF8 (ORF8 SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    169395-plasmid-map-sequence-id-336176

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  19. SARS2_HisSUMOTEV-nsp7/nsp8

    Plasmid
    #234405
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Inserts
    Nsp7_SARS2
    Nsp8_SARS2
    Tags
    His6-SUMO-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    234405-plasmid-map-sequence-id-469465

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  20. MDH2_EC

    Plasmid
    #224146
    Purpose
    IPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.
    Depositor
    Katherine Walstrom
    Insert
    MDH-2 (mdh-2 Nematode)
    Tags
    His tag, Xpress tag, enterokinase cleaveage site
    Expression
    Bacterial
    Available Since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224146-plasmid-map-sequence-id-444697

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Showing: 5621 - 5640 of 29873 results