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R701-X58-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61R

Plasmid

#159566

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61R

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61R (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

Q61R

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-355 C-Tag Venus173/155[2] L10 {Esp3I(2x)} mCherry

Plasmid

#129858

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Venus

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-356 N-Tag Venus173/155[2] L10 {Esp3I(2x)} mCherry

Plasmid

#129859

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Venus

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-357 C-Tag Venus173/155[2] L20 {Esp3I(2x)} mCherry

Plasmid

#129860

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Venus

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-358 N-Tag Venus173/155[2] L20 {Esp3I(2x)} mCherry

Plasmid

#129861

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Venus

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-360 N-Tag Venus173/155[2] L32 {Esp3I(2x)} mCherry

Plasmid

#129863

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Venus

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-359 C-Tag Venus173/155[2] L32 {Esp3I(2x)} mCherry

Plasmid

#129862

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Venus

Expression

Mammalian

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Thy-1-CRISPR-gRNA#2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124284

Purpose

sgRNA against human Thy-1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)

Use

CRISPR

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

Plasmid

#68405

Purpose

The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter

Depositor

Jannik Elverløv-Jakobsen

Inserts

3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
FlpO

Use

CRISPR

Tags

mTurquoise2 (BFP)

Expression

Mammalian

Promoter

Synthetic Probasin ARRx2 and U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

Plasmid

#180280

Purpose

The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (backbone) and BFP

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Plasmid

#177919

Purpose

Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

Plasmid

#159849

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter TCF (1)(2) sites mutant pGL3Basic

Plasmid

#32734

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase

Mutation

-75 TCF(1) and -68 TCF(2) - CTTTGATCTTTGCT deleti…

Promoter

-962 CCND1

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
TL1 alphaDdCBE A1 (DddA6-C) - TL1 alphaL_DdCBE 2 (left, DddA11-C)

Plasmid

#221994

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A | DddA: T1413I

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(-)-GPI

Plasmid

#184335

Purpose

Expression of GPI-anchored form of mouse Nrx3-alpha containing the alternative splice site 5 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,3,

Depositor

Peter Scheiffele

Article

Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

Insert

Nrxn3-alpha-GPI (Nrxn3 Mouse)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75234

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-2/3-3'

Plasmid

#117325

Purpose

CRISPR-Cas9 for miR-124-2/3, 3'

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

gRNA miR-124-2/3-3' (MIR124-3 Synthetic)

Tags

3xFLAG

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1/2-5'

Plasmid

#117322

Purpose

CRISPR-Cas9 for miR-124-1/2, 5'

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

gRNA miR-124-1/2-5' (MIR124-1 Synthetic)

Tags

3xFLAG

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75238

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Helios-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75246

Purpose

CRISPR/Cas9 NICKASE plasmid against human Helios (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Helios (IKZF2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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