We narrowed to 10,386 results for: Coli
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Plasmid#62080PurposeExpression of human p53 (1-90) (72P) in e. coliDepositorInsertp53 (TP53 Human)
TagsHisExpressionBacterialMutationcontains TAD/PP residues 1-90; 72PPromoterT7Available SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-K292A
Plasmid#134575PurposeExpress human DDX43 mutant (K292A, ATPase dead) in E. coliDepositorInsertDDX43-K292A (DDX43 Human)
TagsHisExpressionBacterialMutationfull-length (K292A)PromoterT7Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b-Strep-hAGT-His
Plasmid#167276PurposeOverexpression of the human o_-alkylguanine-dna alkyltransferase in E. coliDepositorAvailable SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-N
Plasmid#128416PurposeExpress INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
TagsGSTExpressionBacterialMutationN-terminal region (1–513 aa)Promotertac promoterAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-C
Plasmid#128417PurposeExpress INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
TagsGSTExpressionBacterialMutationINTS3 C-terminal region (514-1042 aa)PromoterTac promoterAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-HsSyx3
Plasmid#179288PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage siteDepositorInsertSyx
TagsHis6 + SUMO + TEV protease cleavage siteExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7Available SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINF Ta-sro1 WWE-PARP
Plasmid#192550PurposeE. coli expression vector for Triticum aestivum sro1 WWE-PARP domains (amino acids 2-434)DepositorInsertSIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
TagsHis6, 3C protease cleavage siteExpressionBacterial, Insect, and Mamm…PromoterT7 promoterAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28A-p53 (1-73) (P12A P13A)
Plasmid#62066PurposeExpression of human p53 (residues 1-73) (P12A, P13A) in e. coliDepositorInsertp53 (TP53 Human)
TagsHisExpressionBacterialMutationContains TAD residues 1-73; P12A, P13APromoterT7Available SinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
PGEX-DDX21-D2+C
Plasmid#179243Purposeprotein purification in E. coliDepositorAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGHa-SERBP1(189-400)
Plasmid#172315PurposeExpression of the C-terminal (189-400) domain of SERBP1 in E. coli.DepositorInsertPlasminogen Activator Inhibitor 1 RNA-binding protein, residues 189-400 (SERBP1 Human)
TagsHis8ExpressionBacterialMutationdeleted 1-188, 401-408Available SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28A-p53 (1-73) (P to A)
Plasmid#62069PurposeExpression of human p53 (1-73) (all P to A) in e. coliDepositorInsertp53 (TP53 Human)
TagsHisExpressionBacterialMutationContains TAD residues 1-73; all Prolines changed …PromoterT7Available SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28A-p53 (1-73) (P27A)
Plasmid#62068PurposeExpression of human p53 (1-73) (P27A) in e. coliDepositorInsertp53 (TP53 Human)
TagsHisExpressionBacterialMutationContains TAD residues 1-73; P27APromoterT7Available SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
B8-pVTCB052
Plasmid#231302PurposePlasmid used for expression of AI-designed GFP protein B8 in E. coliDepositorInsertB8
ExpressionBacterialAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJExpress401-KDAC8 H143A
Plasmid#224342PurposeExpresses human KDAC8 (HDAC8) H143A in E. coliDepositorInsertKDAC8 H143A (HDAC8 Human)
TagsTEV-cleavable His6ExpressionBacterialMutationHistidine 143 mutated to alaninePromoterT5Available SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28:LanIV-Chondromyces
Plasmid#225078PurposeExpresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIV-Chondromyces)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-56
Plasmid#225073PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-56)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanI-101
Plasmid#225065PurposeExpresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanI-101)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2E
Plasmid#225072PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2E (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2E)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2D
Plasmid#225071PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2D (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2D)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP
Plasmid#166150PurposeExpression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coliDepositorInsertDDX5ΔPrD(1-535)
TagsHis10-Smt3 and SNAPExpressionBacterialMutationΔPrD(1-535)PromoterT7Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-DDX5ΔIDR(kf)-SNAP
Plasmid#166151PurposeExpression of N. furzeri DDX5ΔIDR(1-483) mutant in E. coliDepositorInsertDDX5ΔIDR(1-483)
TagsHis10-Smt3 and SNAPExpressionBacterialMutationΔIDR(1-483)PromoterT7Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-hMICU1EF
Plasmid#199180PurposeThis plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.DepositorInsertMICU1 (MICU1 Human)
TagsHis6 and MBP, Thrombin site, TEV siteMutationD421A, E432K, F433W, F453WAvailable SinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1TMSNKRS
Plasmid#198323PurposetRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-MerTK_KRmutant-PTPN1
Plasmid#196447PurposeExpression of MerTK Kinase Domain in E. coli and surface entropy reduction mutant for crystallization, coexpress PTPN1DepositorExpressionBacterialMutationK591R, K639R, K702R, K865RPromoterT7 promoterAvailable SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPINF Ta-sro1 PARP
Plasmid#192549PurposeE. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)DepositorInsertSIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)
TagsHis6, 3C protease cleavage siteExpressionBacterial, Insect, and Mamm…PromoterT7 promoterAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-CITED(216-242)+CBPTAZ1(340-439)
Plasmid#189961PurposeCoexpression of CBP TAZ1 and CITED in E coliDepositorExpressionBacterialPromoterT7Available SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
H6GB1-CITED2(216-269APAA) + CBP TAZ1(340-439)
Plasmid#189963PurposeCoexpression of CBP TAZ1 and CITED2 in E coliDepositorExpressionBacterialMutationL243A, E245A, L246APromotert7Available SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CITED2(216-246)+CBP TAZ1(340-439)
Plasmid#189962PurposeExpression of CBP TAZ1 and CITED2 in E coliDepositorExpressionBacterialPromotert7Available SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-HsSyx3
Plasmid#179289PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + GFP + TEV protease cleavage siteDepositorInsertSyx
TagsHis6 + GFP + TEV protease cleavage siteExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7Available SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHsSyx3-MBP
Plasmid#179286PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6DepositorInsertSyx
TagsTEV protease cleavage site + mature maltose bindi…ExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7Available SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only