We narrowed to 16,201 results for: genome
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Plasmid#188449PurposeExpression of eSpCas9 tagged with ZsgreenDepositorTypeEmpty backboneUseCRISPR and LentiviralPromoterEF1-alphaAvailable SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only
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psiCHECK2-2A-3xFLAG-SBP-2A
Plasmid#159584PurposeLuciferase reporter for ribosome pausing controlDepositorInserts2A-3xFLAG-SBP-2A
EIF2S3 5UTR
UseLuciferaseExpressionMammalianAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI Cas9 H2B-RFP
Plasmid#92397PurposeCAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRESDepositorInsertCas9
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFlox_N-Nipbl_Neo_FKBPF36V_mScarlet
Plasmid#163883PurposeAcute Depletion NIPBL-mScarletDepositorInsertNipbl (Nipbl Mouse)
ExpressionMammalianAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC234r-ccdB/TPK2
Plasmid#176179Purposeyeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)DepositorInsertdropout cassette containing toxic genes ccdB and TPK2
UseSynthetic BiologyAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pSPneogRNA241510+MT
Plasmid#84292PurposeExpress LdPBK_241510.1 and LdMT targeting gRNAs simutaneouslyDepositorInsertLdBPK_241510.1 targeting gRNA and LdMT targeting gRNA
UseCRISPR; Leishmania donovaniPromoterL. donovani ribosome RNA promoterAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
AsdCas12a VPR
Plasmid#188512PurposeExpresses FLAG tagged AsdCas12a VPRDepositorInsertdCas12a
UseCRISPR and LentiviralExpressionMammalianMutationD908APromoterEF1aAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-KRAS-G12C-tmpknot-epegRNA
Plasmid#214094PurposeLentiviral vector expressing epegRNA to induce KRAS G12C mutationDepositorInsertKRAS G12C epegRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
CD200RCD4d3+4-bio-linker-His
Plasmid#36153PurposeA positive control receptor bait with bio-linker-His (rat CD4d3+4-bio-linker-his) and prey (rat CD200 receptor) EXPRESs plasmidDepositorInsertCD200R (Cd200r1 Rat)
TagsHis tag, biotinylation peptide, and ratCD4d3+4ExpressionMammalianPromoterCMVAvailable SinceMay 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-mEGFP-NLS
Plasmid#205005PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS3-mEGFP-NLS
Plasmid#205004PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
UseTardigrade expressionAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB2988
Plasmid#63638PurposepCfB2988 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Donor_eGP2AP_RC
Plasmid#133784PurposeLibrary reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.DepositorTypeEmpty backboneTagseGFP-P2A-PuroExpressionMammalianAvailable SinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_TOP1
Plasmid#183323PurposeAll-in-One CRISPRko system with a guide RNA that targets TOP1 geneDepositorInsertTOP1
UseLentiviralAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
ePPEmax*
Plasmid#205244PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRExpressionPlantMutationDetailed in manuscriptAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICSL12009
Plasmid#68257PurposeLevel 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)DepositorInsertPromoter and 5UTR Ubiquitin (Zea mays)
UseSynthetic BiologyAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
ePPE-V223A
Plasmid#205243PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertSV40_NLS-nCas9-NC-MLV-SV40_NLS
UseCRISPRExpressionPlantMutationDetailed in manuscriptAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-STMN1
Plasmid#31326DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcU6_1 sgRNA
Plasmid#92395PurposeChicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.DepositorInsertchick U6.1 promoter and gRNA cloning cassette
UseCRISPRExpressionMammalianPromoterchick U6.1Available SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only