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mV-MxCBD_655

Plasmid

#105823

Purpose

Expresses mVenus-fusion protein of MxCBD (Myxococcus xanthus CarH)

Depositor

Harald Janovjak

Article

Kainrath et al Angew Chem Int Ed Engl. 2017 Apr 10;56(1

Insert

mV-MxCBD

Expression

Mammalian

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGL2_136

Plasmid

#199083

Purpose

Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_137

Plasmid

#199084

Purpose

Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-EMX1+38

Plasmid

#140579

Purpose

Expresses a pegRNA in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

pegRNA EMX1 +38

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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bfloGFPc1a

Plasmid

#60750

Purpose

Bacterial expression of Amphioxus GFPc1

Depositor

Dimitri Deheyn

Article

Bomati et al Sci Rep. 2014 Jun 27;4:5469. doi: 10.103

Insert

GFPc1

Tags

8xHIS

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2014

Availability

Academic Institutions and Nonprofits only
p213_LTJ_160bpCD90.1Template

Plasmid

#82666

Purpose

160bp HDR template for murine CD90.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

160bp genomic DNA sequence from murine CD90.1

Use

Hdr template for gene editing

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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p230_LTJ_4kbCD90.1Template

Plasmid

#82669

Purpose

4kb HDR template for murine CD90.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

4kb genomic DNA sequence from murine CD90.1

Use

Hdr template for gene editing

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMVd2-Dr-TrkA

Plasmid

#127956

Purpose

Myr DrTrkB expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al Nat Commun. 2019 Mar 8;10(1):1129. doi:

Insert

Myr-3xSAG-Dr-hel4-TrkA

Expression

Mammalian

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pBSII-flotillin1c

Plasmid

#62801

Purpose

enriched expression in dorsal neural tube

Depositor

Sally Moody

Article

Pandur et al Dev Dyn. 2004 Dec;231(4):881-7.

Insert

flotillin 1c (flot1-b Frog)

Expression

Bacterial

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pmCherry-U6-VEGFA

Plasmid

#140588

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA VEGFA

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pABCb10aa1-70-GFP

Plasmid

#19038

Depositor

Orian Shirihai

Article

Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-6

Insert

amino acids 1 - 70 of the targeting sequence of ABCb1

Tags

GFP

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pU6-pegRNA-DNMT1+38

Plasmid

#140578

Purpose

Expresses a pegRNA in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

pegRNA DNMT1 +38

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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PSD95-TS:YSOG1

Plasmid

#42226

Depositor

Roger Tsien

Article

Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

Insert

PSD95

Tags

TS:YSOG1

Expression

Mammalian

Available Since

Feb. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pFloxin-IRES-Ofd1-Myc

Plasmid

#24559

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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pRB30

Plasmid

#165480

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-FLAG-10x His

Expression

Mammalian

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMLS271

Plasmid

#73722

Purpose

SapTrap SNAPtag + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Snaptag + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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p242_LTJ_1kbCD45.1Template

Plasmid

#82661

Purpose

1kb HDR template for murine CD45.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

1kb genomic DNA sequence from murine CD45.1

Use

Hdr template for gene editing

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-Dr-TrkA

Plasmid

#127963

Purpose

Myr mCherryDrTrkA expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al Nat Commun. 2019 Mar 8;10(1):1129. doi:

Insert

Myr-mCherry-3xSAG-Dr-hel-4- TrkA

Expression

Mammalian

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCZGY2141

Plasmid

#87757

Purpose

Pcol-19-PH::miniSOG, expression of membrane targeted PH-miniSOG in epidermis.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pcol-19-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Pcol-19

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pET16b-Cas2

Plasmid

#122286

Purpose

Cas2 cloned in pET16b

Depositor

Jennifer Doudna

Article

Nunez et al Nat Struct Mol Biol. 2014 Jun;21(6):528-

Insert

Eco Cas2

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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