We narrowed to 59,538 results for: cloning

1. pTYC2_pPromALB_enh29774_FireflyLuc

   Plasmid

   #220286

   Purpose

   Firefly luciferase with mouse liver DNase hypersensitive site # 29774 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

   Depositor

   David Waxman

   Article

   Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

   Insert

   mouse liver DNase hypersensitive site # 29774
   

   Use

   Luciferase

   Promoter

   minimal albumin promoter

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTYC3_pPromALB_enh50363_FireflyLuc

   Plasmid

   #220287

   Purpose

   Firefly luciferase with mouse liver DNase hypersensitive site # 50363 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

   Depositor

   David Waxman

   Article

   Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

   Insert

   mouse liver DNase hypersensitive site # 50363
   

   Use

   Luciferase

   Promoter

   minimal albumin promoter

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTYC4_pPromALB_enh50358_FireflyLuc

   Plasmid

   #220288

   Purpose

   Firefly luciferase with mouse liver DNase hypersensitive site # 50358 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

   Depositor

   David Waxman

   Article

   Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

   Insert

   mouse liver DNase hypersensitive site # 50358
   

   Use

   Luciferase

   Promoter

   minimal albumin promoter

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPOM034_P7_3"Lys3

   Plasmid

   #216459

   Purpose

   3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   3''lys3
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPOM038_P8b_5'Lys3

   Plasmid

   #216463

   Purpose

   5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   5'lys3
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Available Since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TCF4 - P381L in pENTR

   Plasmid

   #216570

   Purpose

   gateway cloning donor vector containing TCF4 ASD mutation

   Depositor

   Douglas Allan, Christopher Loewen

   Insert

   TCF4 (TCF4 Human)
   

   Use

   Gateway entry plasmid

   Mutation

   P381L

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TCF4 - D424N in pENTR

   Plasmid

   #216571

   Purpose

   gateway cloning donor vector containing TCF4 ASD mutation

   Depositor

   Douglas Allan, Christopher Loewen

   Insert

   TCF4 (TCF4 Human)
   

   Use

   Gateway entry plasmid

   Mutation

   D424N

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223_NKX2-1_Long

   Plasmid

   #221471

   Purpose

   Gateway cloning of NKX2-1 long isoform overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR223_NKX2-1_W208L

   Plasmid

   #221474

   Purpose

   Gateway cloning of NKX2-1 W208L DNA binding mutation overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Gateway donor vector

   Mutation

   W208L

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223_NKX2-1_V205F

    Plasmid

    #221473

    Purpose

    Gateway cloning of NKX2-1 V205F DNA binding mutation overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Gateway donor vector

    Mutation

    V205F

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223_NKX2-1

    Plasmid

    #221472

    Purpose

    Gateway cloning of NKX2-1 short isoform overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPOM040_P8b_5'Ura4

    Plasmid

    #216461

    Purpose

    5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    5'ura4
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPOM036_P7_3"Ura4

    Plasmid

    #216465

    Purpose

    3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    3''ura4
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPOM024_P2_pMPA1

    Plasmid

    #216449

    Purpose

    Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter mpa1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPOM022_P2_pRPS1002

    Plasmid

    #216447

    Purpose

    Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter rps1002
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPOM020_P2_pTRS1

    Plasmid

    #216445

    Purpose

    Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter trs1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPOM014_P2_pGPM1

    Plasmid

    #216439

    Purpose

    Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter gpm1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Available Since

    May 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPOM042_P15678_MultiGeneBackbone

    Plasmid

    #216467

    Purpose

    Empty integration vector for genomic integration for multigene into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPOM041_P15678_SingleGeneBackbone

    Plasmid

    #216466

    Purpose

    Empty integration vector for genomic integration of 1 transcriptional unit into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPOM032_P4_tSynth30

    Plasmid

    #216457

    Purpose

    Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Terminator synth30
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge