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FRas2-M Acceptor

Plasmid

#45149

Depositor

Ryohei Yasuda

Article

Oliveira et al PLoS One. 2013;8(1):e52874. doi: 10.1371

Insert

mRFP - Raf binding domain-mRFP

Tags

mRFP

Expression

Mammalian

Mutation

lysine 65 changed to glutamine, lysine 108 mutate…

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
BSP611

Plasmid

#122256

Purpose

expression vector for mtagRFP-T under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mtagRFP-T

Expression

Worm

Promoter

EFT3

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAG506-mito-frFAST

Plasmid

#160057

Purpose

Expresses mito-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

Mito-frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSKΔB-TRE-dCas9-EGFP

Plasmid

#62347

Purpose

Plasmid for tet-inducible expression of dCas9-EGFP in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

dCas9-EGFP

Use

CRISPR

Tags

HA and SV40 NLS

Expression

Mammalian

Promoter

TRE

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-miniE

Plasmid

#181987

Purpose

MiniE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

TfR1-MiniE

Expression

Mammalian

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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JAB2121

Plasmid

#190343

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (empty vector control)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Type

Empty backbone

Expression

Plant

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-BREP

Plasmid

#172340

Purpose

AAV transfer plasmid for BREP (bioluminescent red protein)

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BREP

Use

AAV

Promoter

hSyn promoter

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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BSP606

Plasmid

#122251

Purpose

expression vector for mECitrine under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mECitrine

Expression

Worm

Promoter

EFT3

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAG499-FRB-N-frFAST

Plasmid

#160059

Purpose

Expresses FRB-N-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

FRB-N-frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJL005

Plasmid

#92327

Purpose

Express FtsZ tagged with mEos3.2 in E coli

Depositor

Jie Xiao

Article

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

FtsZ

Tags

mEos3.2

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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CyRFP1-C1

Plasmid

#84354

Purpose

Expresses CyRFP1 fluorescent protein in an empty backbone

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

CyRFP1

Expression

Mammalian

Mutation

H61M (compared with CyOFP)

Promoter

CMV

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pXL-CAG-3XAP-NLG1

Plasmid

#43923

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

3XAP-NLG1

Tags

HA

Expression

Mammalian

Promoter

Chicken Beta Actin

Available Since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-mLanYFP-polyA

Plasmid

#174021

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

mLanYFP

Use

Zebrafish knock-in tagging

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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FEM1C

Plasmid

#167842

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

FEM1C (FEM1C Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGKO2

Plasmid

#63617

Purpose

a binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants.

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCY 3080-01

Plasmid

#36226

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yEmCitrine

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEmCitrine-kanMX

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pXL-CAG-ICeuI-sHRPaNRX3beta

Plasmid

#79910

Purpose

piggyBac transposon vector, sHRPaNRX3beta

Depositor

Joshua Sanes

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPaNRX3beta

Use

Piggybac

Expression

Mammalian

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pa-G-GECO1.2

Plasmid

#67622

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

pa-G-GECO1.2

Expression

Mammalian

Mutation

V81H, M84K, L189F, T190S

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only
pAM3558

Plasmid

#40252

Depositor

Susan Golden

Article

Chen et al Plasmid. 2008 May;59(3):176-92. Epub 200

Insert

Gm cassette (BssHII+SphI)

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pCY 3110-01

Plasmid

#36236

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

mEos2

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-mEos2-kanMX

Available Since

June 19, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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