We narrowed to 7,267 results for: trac

1. pET28a(cd226)

   Plasmid

   #177900

   Purpose

   Bacterial expression of his10-tagged human cd226 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   CD226 (aa 288-336 only) (CD226 Human)
   

   Use

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson

   Plasmid

   #117662

   Purpose

   Test intracellular localisation of E2-Crimson and study the secretion efficiency

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pOst1-pro-af(MUT1)-E2-Crimson
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   This plasmid encodes the fluorescent protein E2-C…

   Promoter

   pAOX1

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

   Plasmid

   #87827

   Purpose

   Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

   Inserts

   NLS
   PTS1
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter, tetracycline operator

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5-FRT-TO-FH-Nsp13_GC3opt

   Plasmid

   #157715

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp13_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pSW002-Pc-TorT(sp)-mNeonGreen

   Plasmid

   #205021

   Purpose

   Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   TorT-mNeonGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   Pc

   Available since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX4T3-HA-ERK2 D321N

   Plasmid

   #53202

   Purpose

   Expresses GST and HA tagged human ERK2 D321N from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK2 (MAPK1 Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   D321N

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pLKO_AU1.HSV.S_mCherry-NLS

   Plasmid

   #178224

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   AU1.HSV.S and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   eF1a

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO_HA.NWS.Ollas_mCherry-NLS

   Plasmid

   #178263

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HA.NWS.Ollas and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   eF1a

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a(His10-PD-1cyto)

   Plasmid

   #177896

   Purpose

   E coli expression of His10 tag fused to human PD-1 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   PD-1 (aa 194-288 only) (PDCD1 Human)
   

   Use

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a(CTLA4)

    Plasmid

    #177905

    Purpose

    Bacterial expression of his10-tagged human CTLA4 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    CTLA4 (aa 183-223 only) (CTLA4 Human)
    

    Use

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO_NWS.HA.V5_mCherry-NLS

    Plasmid

    #178283

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    NWS.HA.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCEP4-myc-EphB1

    Plasmid

    #200981

    Purpose

    Mammalian expression plasmid for myc-tagged EphB1

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EphB1 (EPHB1 Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO_AU1.VSVg.V5_mCherry-NLS

    Plasmid

    #178236

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.VSVg.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO_AU1.S.VSVg_mCherry-NLS

    Plasmid

    #178234

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.S.VSVg and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO_C.HA.V5_mCherry-NLS

    Plasmid

    #178242

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HA.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV-tetO-Elf5

    Plasmid

    #70772

    Purpose

    Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Elf5

    Depositor

    Hubert Schorle

    Article

    Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

    Insert

    Elf5 (Elf5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-tetO-Id2

    Plasmid

    #70764

    Purpose

    Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Id2

    Depositor

    Hubert Schorle

    Article

    Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

    Insert

    inhibitor of DNA binding 2 (Id2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA-FRT-TO-Nsp7-Nsp8_GC3opt

    Plasmid

    #157691

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. FUW TetO HA-AMPKa1 AS

    Plasmid

    #69825

    Purpose

    Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV promoter with tetracycline operator

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO_AU1.HA.VSVg_mCherry-NLS

    Plasmid

    #178220

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.HA.VSVg and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge