We narrowed to 19,002 results for: Tec

1. FUW-tetO-NFIA

   Plasmid

   #141403

   Purpose

   Inducible Expression of NFIA

   Depositor

   Lorenz Studer

   Article

   Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

   Insert

   NFIA (NFIA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-GRAB_eCB2.0

   Plasmid

   #164604

   Purpose

   Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid sensor GRAB_eCB2.0
   

   Use

   AAV

   Mutation

   S383T

   Promoter

   hSyn

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-Sp-gRNA-AAVS1_B3903c_attP

   Plasmid

   #182144

   Purpose

   To insert attP attachment site at AAVS1 in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   AAVS1_B3903c_attP pegRNA
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-Sp-gRNA-AAVS1_A3786c_attP

   Plasmid

   #182143

   Purpose

   To insert attP attachment site at AAVS1 in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   A3786c-attP pegRNA
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-ExRai-CKAR

   Plasmid

   #118409

   Purpose

   cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-CKAR
   

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB505

   Plasmid

   #74847

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-hLbCpf1(TYCV) (pY230)

   Plasmid

   #89355

   Purpose

   Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   G532R/K595R

   Promoter

   CMV

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. HypoxCR

   Plasmid

   #59946

   Purpose

   A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.

   Depositor

   Anne Le

   Article

   Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p

   Inserts

   GFP-Pest
   mCherry-Geminin
   

   Use

   Lentiviral

   Tags

   FLAG and PEST

   Expression

   Mammalian

   Promoter

   CMV and min CMV

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-Cas9-NLS-6xHis

   Plasmid

   #62933

   Purpose

   Expression of Cas9-NLS-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9-NLS-6xHis
   

   Tags

   6xHis tag and NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC gRNA Shuttle

    Plasmid

    #47024

    Purpose

    Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Insert

    gRNA Shuttle
    

    Use

    CRISPR; Cas9

    Expression

    Plant

    Mutation

    G to T cloning mutation at position 323

    Promoter

    Medicago truncatula U6.6

    Available Since

    Sept. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. CP1028-ABE8e

    Plasmid

    #138492

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSpCas9 (CP1028)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET29b-AK1-His

    Plasmid

    #124135

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Adenylate kinase (AK1 Chicken)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-APOBEC1-YTH

    Plasmid

    #131636

    Purpose

    Expresses APOBEC1 fused to the YTH domain

    Depositor

    Kate Meyer

    Article

    Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

    Insert

    APOBEC1 fused to YTH domain (YTHDF2 Human, Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHLUM

    Plasmid

    #40276

    Depositor

    Markus Ralser

    Article

    Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

    Inserts

    LEU2 (LEU2 Budding Yeast)
    URA3 (URA3 Budding Yeast)
    MET15/17 (MET17 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    endogenous LEU2, endogenous MET15/17, and endogen…

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-ExRai-CKAR(T/A)

    Plasmid

    #118410

    Purpose

    Negative-control mutant for ExRai-CKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-CKAR
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pShuttle

    Plasmid

    #192871

    Purpose

    Gateway compatible vector with BsaI cloning site

    Depositor

    Thorsten Stiewe

    Article

    Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

    Type

    Empty backbone

    Use

    Gateway-compatible cloning vector

    Available Since

    Dec. 12, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. AAVS1-TRE-SHH

    Plasmid

    #134350

    Purpose

    Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus

    Depositor

    Lorenz Studer

    Article

    Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.

    Insert

    SHH (SHH Human)
    

    Expression

    Mammalian

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT281

    Plasmid

    #122611

    Purpose

    express evoAPOBEC1-BE4max in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoAPOBEC1-BE4max
    

    Expression

    Mammalian

    Mutation

    E4K H109N H122L D124N R154H A165S P201S F205S

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMV_AA088

    Plasmid

    #216104

    Purpose

    Knockout, EF1a-driven Cas9 (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-Cas9-6xHis

    Plasmid

    #62374

    Purpose

    Expression of Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9_6xHis
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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