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We narrowed to 10,081 results for: crispr plasmids

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Showing: 5701 - 5720 of 10081 results

  1. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid
    #174136
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174136-plasmid-map-sequence-id-341914

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  5. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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  6. eSpCas9(1.1)_NoFlag_hROSA26_Left

    Plasmid
    #191442
    Purpose
    Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hROSA26 sgRNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191442-plasmid-map-sequence-id-378118

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  7. pLenti-hNC-gRNA2

    Plasmid
    #249201
    Purpose
    sgRNA control
    Depositor
    Weihong Tan
    Insert
    Non-Targeting
    Use
    CRISPR and Lentiviral
    Promoter
    U6
    Available Since
    April 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    249201-plasmid-map-sequence-id-499744

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  8. pLenti-hNC-gRNA1

    Plasmid
    #249200
    Purpose
    sgRNA control
    Depositor
    Weihong Tan
    Insert
    Non-Targeting
    Use
    CRISPR and Lentiviral
    Promoter
    U6
    Available Since
    April 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    249200-plasmid-map-sequence-id-499733

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  9. pLenti-hNC-gRNA5

    Plasmid
    #249204
    Purpose
    sgRNA control
    Depositor
    Weihong Tan
    Insert
    Non-Targeting
    Use
    CRISPR and Lentiviral
    Promoter
    U6
    Available Since
    April 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    249204-plasmid-map-sequence-id-506545

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  10. pLenti-hNC-gRNA7

    Plasmid
    #249206
    Purpose
    sgRNA control
    Depositor
    Weihong Tan
    Insert
    Non-Targeting
    Use
    CRISPR and Lentiviral
    Promoter
    U6
    Available Since
    Feb. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    249206-plasmid-map-sequence-id-499748

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  11. pLenti-hNC-gRNA6

    Plasmid
    #249205
    Purpose
    sgRNA control
    Depositor
    Weihong Tan
    Insert
    Non-Targeting
    Use
    CRISPR and Lentiviral
    Promoter
    U6
    Available Since
    Feb. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    249205-plasmid-map-sequence-id-499747

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  12. pLenti-hNC-gRNA4

    Plasmid
    #249203
    Purpose
    sgRNA control
    Depositor
    Weihong Tan
    Insert
    Non-Targeting
    Use
    CRISPR and Lentiviral
    Promoter
    U6
    Available Since
    Feb. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    249203-plasmid-map-sequence-id-499746

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  13. pLenti-hNC-gRNA3

    Plasmid
    #249202
    Purpose
    sgRNA control
    Depositor
    Weihong Tan
    Insert
    Non-Targeting
    Use
    CRISPR and Lentiviral
    Promoter
    U6
    Available Since
    Feb. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    249202-plasmid-map-sequence-id-499745

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  14. 1197R_gZBF-ER

    Plasmid
    #241825
    Purpose
    gRNA expressing plasmid with broken SEPARATOR
    Depositor
    Omar Akbari
    Insert
    Actin5C promoter
    Use
    CRISPR
    Expression
    Insect
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    241825-plasmid-map-sequence-id-487606

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  15. pR6K-crRNA-CASTIF

    Plasmid
    #199654
    Purpose
    R6K plasmid with a I-F CAST targeting lacZ
    Depositor
    IIya Finkelstein
    Insert
    CAST I-F systems
    Expression
    Bacterial
    Promoter
    J23119 promoter
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199654-plasmid-map-sequence-id-412255

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  16. pR6K-crRNA-CASTIB

    Plasmid
    #199656
    Purpose
    R6K plasmid with a I-B CAST targeting lacZ
    Depositor
    IIya Finkelstein
    Insert
    CAST I-B systems
    Expression
    Bacterial
    Promoter
    J23119 promoter
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199656-plasmid-map-sequence-id-412263

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  17. pTJK459

    Plasmid
    #138008
    Purpose
    Empty sgRNA expression vector for expression of sgRNA for Sp Cas9
    Depositor
    Tami Kingsbury
    Type
    Empty backbone
    Use
    Lentiviral
    Mutation
    U-A flip and hairpin extension: cgtttAagagctaTGCT…
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138008-plasmid-map-sequence-id-275002

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  18. pCryptDel4.8

    Plasmid
    #141293
    Purpose
    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    RelB
    gRNA targetting pMUT2
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Native promoter from pMUT2 relB/relE operon and P…
    Available Since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    141293-plasmid-map-sequence-id-322822

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Showing: 5701 - 5720 of 10081 results