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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,582 results for: eng

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Showing: 5741 - 5760 of 14582 results

  2. C25-pLAC

    Plasmid
    #45283
    Purpose
    lacritin-intein fusion plasmid coding for truncated lacritin without signal peptide
    Depositor
    Gordon Laurie
    Publication
    Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.
    Insert
    pLAC-C25 (LACRT Human)
    Use
    Protein expression vector
    Tags
    Intein
    Expression
    Bacterial
    Mutation
    contains aa20-113 of lacritin
    Promoter
    T7
    Available since
    Sept. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    45283-plasmid-map-sequence-id-63156

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  7. pHS2096

    Plasmid
    #239835
    Purpose
    NovaIscB E. coli expression for recombinant purification
    Depositor
    Feng Zhang
    Publication
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    NovaIscB
    Tags
    14xHis-TwinStrep-bdSUMO
    Expression
    Bacterial
    Mutation
    Insertion of REC domain from NbaCas9-1 and swap o…
    Promoter
    T7
    Available since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239835-plasmid-map-sequence-id-479109

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  15. ttHsCas12a

    Plasmid
    #182385
    Purpose
    Gives high editing efficiency in barley.
    Depositor
    Wendy Harwood
    Publication
    Lawrenson et al bioRxiv 2022.04.28.489853
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ttHsCas12a
    Use
    CRISPR; Coding sequence only
    Mutation
    D156R
    Promoter
    None
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182385-plasmid-map-sequence-id-361473

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  16. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

    Plasmid
    #181788
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181788-plasmid-map-sequence-id-354819

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  19. pCJ211

    Plasmid
    #162684
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with deletion of the predicted signal peptide (Lys2:Gly19), with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) without 19 residue signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaK2:G19; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162684-plasmid-map-sequence-id-321526

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Showing: 5741 - 5760 of 14582 results