We narrowed to 32,724 results for: eng

1. EGFP-uTEV1-SQSfl

   Plasmid

   #171010

   Purpose

   HiLITR protease with full-length SQS targeting information (ER)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-SQS(FL)
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   pTRE-tight (TetOn)

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pgRNA_CV2

   Plasmid

   #167165

   Purpose

   pgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.

   Depositor

   Alexander Loewer

   Article

   Sheng et al Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVMG0193_Cq-Rpl40-Cas9

   Plasmid

   #169346

   Purpose

   Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

   Depositor

   Valentino Gantz

   Article

   Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

   Insert

   pVMG0193_Cq-Rpl40-Cas9
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   CPIJ002413

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. O-AARS-32

   Plasmid

   #166540

   Purpose

   scFv of a human scaffold targeting Alanyl-tRNA synthetase. Antigen coverage aa 1-968 of 968, full-length

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Promoter

   LacZ

   Available Since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. hsa-cdk2 3'UTR sicheck2

   Plasmid

   #121125

   Purpose

   luciferase assay

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   hsa-cdk2 3'UTR (CDK2 Human)
   

   Expression

   Bacterial

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-30a(+)-N-SH2

   Plasmid

   #111274

   Purpose

   express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
   

   Tags

   HHHHHHENLYFQG

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-Atoh1 S334A

   Plasmid

   #96915

   Purpose

   Murine Atoh1 with S334A mutation

   Depositor

   Albert Edge

   Article

   Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

   Insert

   Atoh1 (Atoh1 Mouse)
   

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   Serine 334 changed to Alanine

   Available Since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-Atoh1 S309A

   Plasmid

   #96916

   Purpose

   Murine Atoh1 with S309A mutation

   Depositor

   Albert Edge

   Article

   Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

   Insert

   Atoh1 (Atoh1 Mouse)
   

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   Serine 309 changed to Alanine

   Available Since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-Atoh1 S325A

   Plasmid

   #96926

   Purpose

   Murine Atoh1 with S325A mutation

   Depositor

   Albert Edge

   Article

   Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

   Insert

   Atoh1 (Atoh1 Mouse)
   

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   Serine 325 changed to Alanine

   Available Since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-Atoh1 S328A

    Plasmid

    #96927

    Purpose

    Murine Atoh1 with S328A mutation

    Depositor

    Albert Edge

    Article

    Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Serine 328 changed to Alanine

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-Atoh1 S331A

    Plasmid

    #96928

    Purpose

    Murine Atoh1 with S331A mutation

    Depositor

    Albert Edge

    Article

    Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Serine 331 changed to Alanine

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-Atoh1 S339A

    Plasmid

    #96929

    Purpose

    Murine Atoh1 with S339A mutation

    Depositor

    Albert Edge

    Article

    Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Serine 339 changed to Alanine

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFBD-Akt13C(WT)

    Plasmid

    #86576

    Purpose

    Expresses cleavable human full-length Akt1(WT)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    3C cleavage site inserted at pos. 130

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. FUP95Q15ADSKGW (D7)

    Plasmid

    #74030

    Purpose

    lentiviral expression of Psd95-Q15A deltaSK-EGFP fusion

    Depositor

    Robert Malenka, Oliver Schluter, Weifeng Xu

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95 (DLG4 Rat)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Q15A, delta SH3-GK (deletion of the fragment betw…

    Promoter

    hUbC

    Available Since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pLJM1 Flag-Sesn2 419A/422A/426A

    Plasmid

    #61872

    Purpose

    Lentivirial expression of Flag-Sesn 419A/422A/426A (mouse) mutant

    Depositor

    Ming Li

    Article

    Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

    Insert

    Sesn2 (Sesn2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    419A/422A/426A

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

16. tdEos-Zyxin-6

    Plasmid

    #57696

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Zyxin (ZYX Human)
    

    Tags

    tdEos

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. mKO-Vinculin-23

    Plasmid

    #57852

    Purpose

    Localization: Focal Adhesions, Excitation: 548, Emission: 561

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Vinculin (VCL Human)
    

    Tags

    mKO

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-hSyn-GRAB_NE1m

    Plasmid

    #123308

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE sensor GRAB_NE1m
    

    Use

    AAV

    Mutation

    contains a glycine-to-threonine mutation at posit…

    Promoter

    hSyn

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-GRAB_NE1h

    Plasmid

    #123309

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE sensor GRAB_NE1h
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-puro-Bcl-XL

    Plasmid

    #46972

    Purpose

    lentiviral expression of Bcl-XL

    Depositor

    Jialiang Wang

    Article

    Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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