We narrowed to 16,201 results for: genome

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pAGK112

Plasmid

#228127

Purpose

Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells

Expression

Mammalian

Promoter

CAG for RT, U6/H1 for ncRNA/gRNA

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ11299: pHR-HSP-ABE8e-hyperdCas12a-EPS-puro

Plasmid

#210605

Purpose

heat shock promoter - activated hyperdCas12a-based transcriptional base editor

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

ABE8e-hyperdCas12a

Use

CRISPR and Lentiviral

Promoter

truncated hsp70

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pICH67131

Plasmid

#153223

Purpose

Level 1 position 1 containing the Nos promoter, NPTII (Kan resistance), and the Nos terminator

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Nos promoter, NPTII, Nos terminator

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTC392

Plasmid

#91223

Purpose

protoplast vector expressing gRNA23 targeting tomato ANT1 (control without TREX2 expression)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting tomato ANT1

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB2792

Plasmid

#63653

Purpose

pCfB2792 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pUBQ10::ZF108_3xFLAG_YPet

Plasmid

#106441

Purpose

pUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter in arabidopsis

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

ZF108_3xFLAG_YPet

Use

Synthetic Biology

Expression

Plant

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_A0402

Plasmid

#91009

Purpose

Module A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

AtUbi10

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pUDE1093

Plasmid

#204227

Purpose

Episomal yeast plasmid expressing the Ercas12a

Depositor

Jean-Marc Daran

Article

Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

Insert

Eubacterium rectale cas12a (Ercas12a)

Expression

Yeast

Mutation

codon optimized for S. cerevisiae

Promoter

Saccharomyces cerevisiae PGK1

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pTX209

Plasmid

#89269

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01 and OsPDS-gRNA02

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsPDS-gRNA01 and OsPDS-gRNA02

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Promoter

Maize Ubiquitin1 promoter

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)_mtZFN(-)2G

Plasmid

#62799

Purpose

mtZFN2G architecture bearing (-) FokI domain

Depositor

Michal Minczuk

Article

Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

Insert

mtZFN(-)2G

Tags

Internal FLAG tag immediately after F1B mitochond…

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)_mtZFN(+)2G

Plasmid

#62798

Purpose

mtZFN2G architecture bearing (+) FokI domain

Depositor

Michal Minczuk

Article

Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

Insert

mtZFN(+)2G

Tags

Internal HA tag immediately after F1B mitochondri…

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_IDH1

Plasmid

#183300

Purpose

All-in-One CRISPRko system with a guide RNA that targets IDH1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

IDH1

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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SL2-sgTelo-MTSa/BFP/pdCas9-C1

Plasmid

#162760

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting telomeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgTelo-MTSa)

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-mRuby3-2A-dCre

Plasmid

#203845

Purpose

Flp-dependent enzymatically inactive Cre and mRuby3 expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dCre

Use

AAV

Tags

mRuby3-2A

Mutation

Enzymatically inactive Cre

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCSN061

Plasmid

#101725

Purpose

Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

Depositor

Rene Verwaal

Article

Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

Inserts

S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
KanMX marker expression cassette.

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Promoter

Heterologous TEF1 promoter from A. gossypii. and …

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pUD628

Plasmid

#103018

Purpose

expression of a Cpf1 programming crRNA targetting ADE2 (crADE2-3.S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crADE2-3.S (ADE2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Pb459-mU6-sgRNA-EF1a-PuroR

Plasmid

#195507

Purpose

piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

Puromycin

Use

CRISPR; Piggybac

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_IDH2

Plasmid

#183301

Purpose

All-in-One CRISPRko system with a guide RNA that targets IDH2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

IDH2

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pFlox_N-p300_Neo_FKBPF36V_mScarlet

Plasmid

#163886

Purpose

Acute Depletion of P300-mScarlet

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

P300 (Ep300 Mouse)

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-EGFR-T790M-tmpknot-epegRNA

Plasmid

#214093

Purpose

Lentiviral vector expressing epegRNA to induce EGFR T790M mutation

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

EGFR T790M epegRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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