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TLS 4: hTLS-CHOP.pBSKS(+)

Plasmid

#21830

Depositor

David Ron

Article

Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

Insert

TLS-CHOP (FUS Human)

Expression

Bacterial

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pFastBac_His-ZZ-TEV-Lis1

Plasmid

#132539

Purpose

Plasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tag

Depositor

Andrew Carter

Article

Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

Insert

Lis1 (PAFAH1B1 Human)

Tags

8x His, Linker-TEV-linker_TEV, and ZZ

Expression

Insect

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-p50(1-435)

Plasmid

#44747

Depositor

Gourisankar Ghosh

Article

Savinova et al Mol Cell. 2009 Jun 12;34(5):591-602. doi

Insert

p50 (Nfkb1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

AA 1-435

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pET16b-Trx C35S

Plasmid

#36947

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Thioredoxin-1 C35S mutation (TXN Human)

Tags

His tag

Expression

Bacterial

Mutation

cys 35 to serine

Promoter

T7

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
EGFP-Nsp15

Plasmid

#165120

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp15 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF57-2xCSTREP

Plasmid

#136217

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF57

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF57

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-(SIM)1

Plasmid

#126954

Purpose

Bacterial expression plasmid for eGFP-(SIM)1 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)

Tags

eGFP

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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GST-PRDM16 1-223

Plasmid

#53346

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 1-223

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
pIC291

Plasmid

#51403

Purpose

Retroviral expression of EGFP-TEV-S-tag SKA1 for protein localization and affinity purification (LAP)

Depositor

Iain Cheeseman

Article

Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

Insert

Ska1 IMAGE 3909951 (SKA1 Human)

Use

Retroviral

Tags

EGFP, S tag, and TEV protease site

Expression

Mammalian

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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Myc-PSFΔRRM1

Plasmid

#35376

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 298-365

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
HA-AMOT p130 delta linker

Plasmid

#32825

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Tags

HA

Expression

Mammalian

Mutation

E5D and amino acids 752-1076 deleted

Promoter

CMV

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
CMV-HA-hTAF6

Plasmid

#17988

Depositor

Naoko Tanese

Article

Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

Insert

TAF6 (TAF6 Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only
FLAG-MED6

Plasmid

#15411

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED6 (Med6 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

Plasmid

#84257

Purpose

Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sp dCas9-ABI
GID1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,Δ42a] mut

Plasmid

#26577

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pmcsg7-mCRT(WT)

Plasmid

#83505

Purpose

Bacterial expression of Calreticulin

Depositor

Malini Raghavan

Article

Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

Insert

CALR (Calr Mouse)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pMDC-pRPS2-HPB

Plasmid

#32077

Depositor

Fumi Katagiri

Article

Qi et al Plant J. 2009 Mar;57(5):932-44. Epub 200

Type

Empty backbone

Use

Gateway destination binary vector

Tags

HA-Prescission-Biotin

Promoter

RPS2 promoter

Available Since

Dec. 6, 2011

Availability

Academic Institutions and Nonprofits only

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hRAD18 dSAP-EGFP

Plasmid

#68825

Purpose

Expresses human RAD18 (deleting SAP domain) tagged with EGFP

Depositor

Satoshi Tateishi

Article

Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

Insert

human RAD18 (deleting SAP domain) tagged with EGFP (RAD18 Human)

Expression

Mammalian

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3 βarr1 S412A Flag

Plasmid

#42195

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

β-arrestin 1 S412A (Arrb1 Rat)

Tags

Flag

Expression

Mammalian

Mutation

S412A

Promoter

CMV

Available Since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-beta-Actin

Plasmid

#37136

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human beta-actin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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