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pECNUS2

Plasmid

#184885

Purpose

ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

Depositor

Eunyoung Chae

Article

Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

RPS5A

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pECNUS3

Plasmid

#184886

Purpose

ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

Depositor

Eunyoung Chae

Article

Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

RPS5A

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pECNUS1

Plasmid

#184884

Purpose

ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

Depositor

Eunyoung Chae

Article

Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

RPS5A

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS1831 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Fah

Plasmid

#199264

Purpose

Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model

Depositor

Erik Sontheimer

Article

Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

Insert

NmeABE8e

Use

AAV and CRISPR

Tags

NLS

Expression

Mammalian

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAct-dCBEevoAPOBEC1

Plasmid

#195514

Purpose

Ubiquitous expression of dCBEevoAPOBEC1 in Drosophila

Depositor

Michael Boutros

Article

Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

Insert

dCBE-evoAPOBEC1

Use

CRISPR

Expression

Insect

Promoter

act5C

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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XAC1 Y82F

Plasmid

#245948

Purpose

Expressing XAC1 with lower turnover rate

Depositor

James Bamburg

Article

Abe et al J Cell Biol. 1996 Mar;132(5):871-85. doi

Insert

cofilin 1 (cfl1) (cfl1.L Frog)

Use

Frog expression

Mutation

Y82F

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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XAC1 WT

Plasmid

#245945

Purpose

Expressing Xenopus ADF/cofilin

Depositor

James Bamburg

Article

Abe et al J Cell Biol. 1996 Mar;132(5):871-85. doi

Insert

cofilin 1 (cfl1) (cfl1.L Frog)

Use

Frog expression

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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XAC1 S3A,Y82F

Plasmid

#245949

Purpose

Expressing constitutively active XAC1 with lower turnover rate

Depositor

James Bamburg

Article

Abe et al J Cell Biol. 1996 Mar;132(5):871-85. doi

Insert

cofilin 1 (cfl1) (cfl1.L Frog)

Use

Frog expression

Mutation

S3A, Y82F

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pUltra BCR-ABL WT

Plasmid

#210432

Purpose

Lentiviral expression of BCR-ABL WT

Depositor

Justin Pritchard

Article

Sokirniy et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

BCR-ABL (ABL1, BCR Human)

Use

Lentiviral

Tags

EGFP-P2A

Promoter

UbC

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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dimerTomato-wGBD

Plasmid

#129635

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

Cdc42 binding domain of N-WASP

Expression

Mammalian

Promoter

CMVdel

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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SL3-MS2-gRNA GG backbone (MOBE3-4)

Plasmid

#219949

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-MS2-SL3 (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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3'MS2-gRNA GG backbone (MOBE1-2)

Plasmid

#219948

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-MS2-3'end (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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Yeast_Target-AID2S

Plasmid

#188647

Purpose

Expressing Target-AID2S in yeasts

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID2S-UGI

Tags

UGI

Expression

Yeast

Mutation

D10A for SpCas9

Promoter

pGal1

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJ2042.2

Plasmid

#140670

Purpose

3-color stripe

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

3-color stripe

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac211-Mup1-mScarlet2I

Plasmid

#140152

Purpose

Plasmid for pop-in/pop-out replacement of Mup1 with Mup1-mScarlet2I

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

MUP1

Expression

Yeast

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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B52 + PIK3R1 sgSTOP

Plasmid

#100714

Purpose

B52 plasmid expressing PI3KR1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting PIK3R1 (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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TEV-VPR

Plasmid

#110200

Purpose

Encodes for HIV-1 VPR fused with TEV protease (TEVp)

Depositor

Sergi Padilla Parra

Article

Jones et al Sci Rep. 2015 Aug 24;5:13449. doi: 10.10

Insert

VPR, TEVp

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCVA-amiR-PDS

Plasmid

#32911

Depositor

Yule Liu

Article

Tang et al Plant Physiol. 2010 Jun;153(2):632-41. E

Insert

amiR-PDS (Nb)

Use

Plant expression

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pET31b-T7-Spinach2-Pl-A

Plasmid

#79160

Purpose

Expresses the Pl-A biosensor under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2-Pl-A

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pNR230

Plasmid

#79546

Purpose

BxbI attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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