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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,609 results for: trac

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Showing: 5761 - 5780 of 7609 results

  3. pHPHS232

    Plasmid
    #228356
    Purpose
    Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d
    Inserts
    mTagBFP2
    Reverse tetracycline transactivator 3
    neo
    Tags
    Blasticidin S deaminase and iCasp9
    Expression
    Mammalian
    Promoter
    TRE3GV and gene trap
    Available Since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228356-plasmid-map-sequence-id-455149

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  7. pDule-Tet2.0

    Plasmid
    #85496
    Purpose
    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044
    Insert
    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32G L65Q F108S Q109D D158S L162N
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85496-plasmid-map-sequence-id-169865

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  8. TFORF0707

    Plasmid
    #143889
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NCOR1 (NCOR1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    143889-plasmid-map-sequence-id-408610

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  13. GST-mDia1(FH1FH2DAD)-His

    Plasmid
    #85822
    Purpose
    contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.
    Depositor
    Antoine Jégou, Guillaume Romet-Lemonne
    Article
    Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc
    Insert
    Protein diaphanous homolog 1 (Diaph1 Mouse)
    Tags
    8*His and GST
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85822-plasmid-map-sequence-id-183287

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  20. pSBtet rtTA G72V SE Δsplice GP Luc

    Plasmid
    #194328
    Purpose
    Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness
    Depositor
    Alexander McLellan
    Article
    Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Tet-responsive promotor related mutations: G72V i…
    Promoter
    Tight tet-responsive promotor
    Available Since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194328-plasmid-map-sequence-id-383152

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Showing: 5761 - 5780 of 7609 results