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Plasmid#177228PurposeExpresses Lkb1(bU6), neomycin (mU6) and non-targeting(hU6) gRNAs and Cre-recombinaseDepositorInsertsgLkb1_2nd/sgNeo1/sgNT1
UseLentiviralTagsExpressionMutationPromoterbU6/mU6/hU6Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdx1-mTQ2-P2A-FlpO-PA
Plasmid#67279PurposePancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon contextDepositorInsertsmTQ2=mturquoise2 blue fluorescent gene
FlpO recombinase
UseTagslinked to FlpO through an P2A ribosomal skipping …ExpressionMammalianMutationPromoterpdxAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ai62(TITL-tdT) Flp-in replacement vector
Plasmid#61576PurposeRecombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locusDepositorInsertchromatin insulator flanked TRE-LSL-tdTomato
UseRecombinase-mediated cassette exchange using flp …TagsExpressionMutationPromoterAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSkipFlox
Plasmid#85797PurposeP. falciparum plasmid for expression of both diCre fragments from single casette using skip peptideDepositorInsertsplit Cre recombinase
UseCre/Lox; P. falciparum expressionTagsFKBP, FRB*ExpressionMutationPromoterPfcrtAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66
Plasmid#168787PurposeDonor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning.DepositorTypeEmpty backboneUseCre/Lox and Synthetic Biology; Expression in fila…TagsExpressionMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRV∆L-5Flpo
Plasmid#182965PurposeThe 3rd generation of rabies vector expressing Flpo recombinaseDepositorInsertFlpo recombinase
UseRabiesTagsExpressionMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQadd2
Plasmid#135662PurposeClostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R.DepositorInsertLox511/66 and LoxFas/71
UseClostridial vector, pamβ1 originTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW1340_pCAG-PV1-PhiC31-N233-L1-FRB-NLS-BGHpA
Plasmid#108789PurposeExpresses N-terminus to aa233 of PhiC31 integrase fused to FRB CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa233
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1353_pCAG-PV1-FKBP-L1-PhiC31-234C-NLS-BGHpA
Plasmid#108790PurposeExpresses aa234 to C-terminus of PhiC31 integrase fused to FKBP CIDDepositorInsertPhiC31 integrase fragment aa234 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2122_pCAG-PV1-GAI-L1-Bxb1-469C-NLS-BGHpA
Plasmid#108816PurposeExpresses aa469 to C-terminus of Bxb1 integrase fused to GAI CIDDepositorInsertBxb1 integrase fragment aa469 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1599_pCAG-PV1-PhiC31-N233-L1-ABI-NLS-BGHpA
Plasmid#108797PurposeExpresses N-terminus to aa233 of PhiC31 integrase fused to ABI CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa233
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1604_pCAG-PV1-PYL-L1-PhiC31-234C-BGHpA
Plasmid#108798PurposeExpresses aa234 to C-terminus of PhiC31 integrase fused to PYL CIDDepositorInsertPhiC31 integrase fragment aa234 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2115_pCAG-PV1-PhiC31-N233-L1-GID1-NLS-BGHpA
Plasmid#108805PurposeExpresses N-terminus to aa233 of PhiC31 integrase fused to GID1 CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa233
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2116_pCAG-PV1-GAI-L1-PhiC31-234C-NLS-BGHpA
Plasmid#108806PurposeExpresses aa234 to C-terminus of PhiC31 integrase fused to GAI CIDDepositorInsertPhiC31 integrase fragment aa234 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1349_pCAG-PV1-PhiC31-N571-L1-FRB-NLS-BGHpA
Plasmid#108795PurposeExpresses N-terminus to aa571 of PhiC31 integrase fused to FRB CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa571
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1362_pCAG-PV1-FKBP-L1-PhiC31-572C-NLS-BGHpA
Plasmid#108796PurposeExpresses aa572 to C-terminus of PhiC31 integrase fused to FKBP CIDDepositorInsertPhiC31 integrase fragment aa572 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSD36_pCAG-PV1-FKBP-L1-TP901-327C-BGHpA
Plasmid#108814PurposeExpresses aa327 to C-terminus of TP901 integrase fused to FKBPDepositorInsertTP901 integrase fragment aa327 to C-terminus
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterpCAGAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSD35_pCAG-PV1-TP901-N326-L1-FRB-NLS-BGHpA
Plasmid#108813PurposeExpresses N-terminus to aa326 of TP901 integrase fused to GID1 CIDDepositorInsertTP901 integrase fragment N-terminus to aa326
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1602_pCAG-PV1-PhiC31-N428-L1-ABI-NLS-BGHpA
Plasmid#108801PurposeExpresses N-terminus to aa428 of PhiC31 integrase fused to ABI CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa428
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1607_pCAG-PV1-PYL-L1-PhiC31-429C-BGHpA
Plasmid#108802PurposeExpresses aa429 to C-terminus of PhiC31 integrase fused to PYL CIDDepositorInsertPhiC31 integrase fragment aa429 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2121_pCAG-PV1-Bxb1-N468-L1-GID1-NLS-BGHpA
Plasmid#108815PurposeExpresses N-terminus to aa468 of Bxb1 integrase fused to GID1 CIDDepositorInsertBxb1 integrase fragment N-terminus to aa468
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSD32_pCAG-PV1-FKBP-L1-TP901-324C-NLS-BGHpA
Plasmid#108812PurposeExpresses aa324 to C-terminus of TP901 integrase with N-term FKBP and C-term NLSDepositorInsertTP901 integrase fragment aa324 to C-terminus
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterpCAGAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSD31_pCAG-PV1-TP901-N323-L1-FRB-NLS-BGHpA
Plasmid#108811PurposeExpresses N-terminus to aa323 of TP901 integrase fused to GID1 CIDDepositorInsertTP901 integrase fragment N-terminus to aa323
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1346_pCAG-PV1-PhiC31-N428-L1-FRB-NLS-BGHpA
Plasmid#108793PurposeExpresses N-terminus to aa428 of PhiC31 integrase fused to FRB CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa428
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1359_pCAG-PV1-FKBP-L1-PhiC31-429C-NLS-BGHpA
Plasmid#108794PurposeExpresses aa429 to C-terminus of PhiC31 integrase fused to FKBP CIDDepositorInsertPhiC31 integrase fragment aa429 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1601_pCAG-PV1-PhiC31-N396-L1-ABI-NLS-BGHpA
Plasmid#108799PurposeExpresses N-terminus to aa396 of PhiC31 integrase fused to ABI CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa396
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1606_pCAG-PV1-PYL-L1-PhiC31-397C-BGHpA
Plasmid#108800PurposeExpresses aa397 to C-terminus of PhiC31 integrase fused to PYL CIDDepositorInsertPhiC31 integrase fragment aa397 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1603_pCAG-PV1-PhiC31-N571-L1-ABI-NLS-BGHpA
Plasmid#108803PurposeExpresses N-terminus to aa571 of PhiC31 integrase fused to ABI CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa571
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1608_pCAG-PV1-PYL-L1-PhiC31-572C-BGHpA
Plasmid#108804PurposeExpresses aa572 to C-terminus of PhiC31 integrase fused to PYL CIDDepositorInsertPhiC31 integrase fragment aa572 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1345_pCAG-PV1-PhiC31-N396-L1-FRB-NLS-BGHpA
Plasmid#108791PurposeExpresses N-terminus to aa396 of PhiC31 integrase fused to FRB CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa396
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only