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Plasmid#115661PurposeEncodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1DepositorInsertGFP and RFP array for MbCas12
UseCRISPRTagsExpressionBacterialMutationPromoterPlacAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti
Plasmid#138318PurposeLenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFPDepositorInsertSauCas9 GFP sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCJH031_proD_LbCas12a-gRNA-GFP_CAM
Plasmid#183075PurposePlasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria.DepositorInsertLbCas12a guide RNA targeting GFP
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF820_U6-sgRNA-EF1a-mCherry2_lenti
Plasmid#138316PurposeLenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFPDepositorInsertSpyCas9 GFP sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
1xNLS-pMJ915v2-sfGFP
Plasmid#88919PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-pMJ915v2-sfGFP
Plasmid#88920PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
CL7_2xNLS_iGeoCas9(C2)_2xNLS_His
Plasmid#222988PurposeBacterial expression of iGeoCas9(C2) constructDepositorInsertCL7_2xNLS_iGeoCas9(C2)_2xNLS_His
UseCRISPRTagsCL7 and HisExpressionBacterialMutationPromotertacAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
gRNA_CloningVector_tagBFP
Plasmid#163747PurposeEmpty gRNA cloning vector, contains a tagBFP fluorescent marker for FACS sorting after transfection.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
gRNA_CloningVector_miRFP670
Plasmid#163748PurposeEmpty gRNA cloning vector, contains a miRFP670 fluorescent marker for FACS sorting after transfection.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL7_2xNLS_iGeoCas9(C1)_2xNLS_His
Plasmid#222987PurposeBacterial expression of iGeoCas9(C1) constructDepositorInsertCL7_2xNLS_iGeoCas9(C1)_2xNLS_His
UseCRISPRTagsCL7 and HisExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T6 Empty target Bxb1
Plasmid#195889PurposeLevel 0 Empty Bxb1 target: attP_Bxb1-BpiI-attB_Bxb1 with golden gate linker in pBLUNT plasmid.DepositorInsertEmpty Bxb1 Target
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T7 Empty target Tp901
Plasmid#195885PurposeLevel 0 Empty Tp901 target: attP_Tp901-BpiI-attB_Tp901 with golden gate linker in pBLUNT plasmid.DepositorInsertEmpty Tp901 Target
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2488
Plasmid#190373PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::IcaR-NLS_19St)DepositorInsertproG1090::IcaR-NLS_19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2487
Plasmid#190372PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-NLS_19St)DepositorInsertproG1090::AmtR-NLS_19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2514
Plasmid#190382PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::TetR-NLS_19St)DepositorInsertproG1090::TetR-NLS_19St
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
T1T/pUBQ10(mTurq->mScarlet)
Plasmid#195947PurposeTp901 target with pUBQ10 switching from mTurq to mScarletDepositorInserttRBCS-mScarlet-attP_Tp901-pUBQ10-attB_Tp901-mTurq-tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
T1B/pUBQ10(mTurq->mScarlet)
Plasmid#195946PurposeBxb1 target with pUBQ10 switching from mTurq to mScarletDepositorInserttRBCS-mScarlet-attP_Bxb1-pUBQ10-attB_Bxb1-mTurq-tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I32 pLBD16 GG0-GG1
Plasmid#195903PurposeLevel 0 pLBD16 promoter with GG0 and GG1 golden gate linkersDepositorInsertpLBD16
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I23 Bxb1 GG1-GG4
Plasmid#195901PurposeLevel 0 Bxb1 integrase with GG1 and GG4 golden gate linkersDepositorInsertBxb1 integrase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I42 Tp901 GG1-GG4
Plasmid#195907PurposeLevel 0 Tp901 integrase with GG1 and GG4 golden gate linkersDepositorInsertTp901 integrae
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB3617
Plasmid#190427PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (pro35S::TetR-NLS-19St)DepositorInsertpro35S::TetR-NLS-19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB3017
Plasmid#190399PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::McbR-NLS-19St)DepositorInsertproG1090::McbR-NLS-19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2858
Plasmid#190394PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-TPL(RD)-19St)DepositorInsertproG1090::AmtR-TPL(RD)-19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2519
Plasmid#190385PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::BetI-NLS_19St)DepositorInsertproG1090::BetI-NLS_19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2518
Plasmid#190384PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::RpaR-NLS_19St)DepositorInsertproG1090::RpaR-NLS_19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2495
Plasmid#190377PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::BetI-VP16_19St)DepositorInsertproG1090::BetI-VP16_19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2494
Plasmid#190376PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::RpaR-VP16_19St)DepositorInsertproG1090::RpaR-VP16_19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2281
Plasmid#190362PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-ERF2(AD)-19St)DepositorInsertproG1090::AmtR-ERF2(AD)-19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2180
Plasmid#190353PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::TetR-VP16-19St)DepositorInsertproG1090::TetR-VP16-19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2156
Plasmid#190345PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-VP16-19St)DepositorInsertproG1090::AmtR-VP16-19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I10 PhiC31 GG2-GG4
Plasmid#195893PurposeLevel 0 PhiC31 integrase with GG2 GG4 golden gate linkersDepositorInsertPhiC31 integrase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I11 PhiC31-NLS GG1-GG4
Plasmid#195894PurposeLevel 0 PhiC31 inegrase NLS with GG1 GG4 Golden gate linkersDepositorInsertPhiC31 integrase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I12 PhiC31-NLS GG2-GG4
Plasmid#195895PurposeLevel 0 PhiC31 inegrase NLS with GG2 GG4 Golden gate linkersDepositorInsertPhiC31 integrase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I14 DST GG4-GG5
Plasmid#195896PurposeLevel 0 DST (destabilization RNA tag: SAUR tag) with GG4 GG5 golden gate linkersDepositorInsertDST
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR
Plasmid#186276PurposePlasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assaysDepositorInsertThiomicrospira shortened CRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSin4 UAP1 F383G
Plasmid#169891PurposeUsed for generation of stable mammalian cell lines expressing UAP1 with with F383G mutationDepositorInsertUDP-N-acetylhexosamine pyrophosphorylase (UAP1 Human)
UseLentiviralTagsNoneExpressionMammalianMutationChanged Phenylalanine (F) at position 383 to glyc…PromoterAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNL1141
Plasmid#160867PurposePJ23110-RBS-PhCFAT-mRuby2-tL3S2P24_KanR_p15ADepositorInsertConiferyl alcohol acyltransferase
UseSynthetic BiologyTagsmRuby2ExpressionBacterialMutationPromoterPJ23110Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b_4CL:P3S + STS:P4S
Plasmid#139789Purposeexpression of biosynthetic enzymes 4CL and STS in fusion with CCDepositorInsertbiosynthetic enzymes 4CL and STS in fusion with coiled-coil segments
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAW218
Plasmid#113240PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flipDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationAAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…PromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD-MTS2 (12)
Plasmid#101698PurposeMTS2 in domestication vectorDepositorInsertCCAT-[MTS from β subunit of Nicotiana plumbaginifolia F1 ATPase]-AATG
UseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only