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  4. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations P676C I730C (NT95)

    Plasmid
    #50913
    Purpose
    Expresses human NKCC1 with mutated Ser and Thr residues in the N-terminus and mutations at P676C I730C. Contains N-terminal 3xFLAG-YFP tag for expression in mammalian cells
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    S150N S155Q S170Q T177A S183R S193E T203A T205A T…
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50913-plasmid-map-sequence-id-76386

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  6. pWR63(YUM1)

    Plasmid
    #203336
    Purpose
    episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    YUM1-targeting sequence
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    203336-plasmid-map-sequence-id-417292

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  7. pWR63

    Plasmid
    #203331
    Purpose
    episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    203331-plasmid-map-sequence-id-409751

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  10. XA106+pRCPam

    Bacterial Strain
    #195858
    Purpose
    E. coli nonsense suppressor strain (leuX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows magenta when CP is induced with rhamnose.
    Depositor
    Gregory Phillips
    Article
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Bacterial Resistance
    Chloramphenicol
    Species
    Acropora millepora
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  11. XA103+pRCPam

    Bacterial Strain
    #195857
    Purpose
    E. coli nonsense suppressor strain (tyrT) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. No color when grown with or without rhamnose.
    Depositor
    Gregory Phillips
    Article
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Bacterial Resistance
    Chloramphenicol
    Species
    Acropora millepora
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  12. XA101+pRCPam

    Bacterial Strain
    #195856
    Purpose
    E. coli nonsense suppressor strain (serU) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows salmon when CP is induced with rhamnose.
    Depositor
    Gregory Phillips
    Article
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Bacterial Resistance
    Chloramphenicol
    Species
    Acropora millepora
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  13. XA92+pRCPam

    Bacterial Strain
    #195855
    Purpose
    E. coli nonsense suppressor strain (glnX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows purple when CP is induced with rhamnose.
    Depositor
    Gregory Phillips
    Article
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Bacterial Resistance
    Chloramphenicol
    Species
    Acropora millepora
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 561 - 580 of 56946 results