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Showing: 561 - 580 of 598 results

  1. pDONR221-SLC9B1_STOP

    Plasmid
    #161389
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC9B1 (NHEDC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161389-plasmid-map-sequence-id-372341

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  2. PKAsub(TA)-TagRFPT-CAAX

    Plasmid
    #87740
    Purpose
    Negative-control PKA substrate fragment for bimFLINC-AKAR, targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub(TA)-TagRFPT-CAAX
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87740-plasmid-map-sequence-id-176201

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  3. pB-TR-TTHA

    Plasmid
    #232479
    Purpose
    Tetracycline inducible PiggyBac vector expressing bacterial TTHA1718 (TTHA) gene gene including flag-tag (DYKDDDDK) on the 3' end
    Depositor
    Lukas Trantirek
    Article
    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1
    Insert
    Thermus thermophilus HB8
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-TetO
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    232479-plasmid-map-sequence-id-471676

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  4. An R57A, R59A CAAX pMT2

    Plasmid
    #206119
    Purpose
    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif
    Depositor
    Annette Dolphin
    Article
    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d
    Insert
    cacna1a (Cacna1a Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    206119-plasmid-map-sequence-id-415935

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  5. An R57A, R59A HA CAAX pMT2

    Plasmid
    #206126
    Purpose
    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif and a HA tag
    Depositor
    Annette Dolphin
    Article
    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d
    Insert
    cacna1a (Cacna1a Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available since
    Oct. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    206126-plasmid-map-sequence-id-412911

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  6. Cav2.2 HA EIVA (E1658A) pCAGGS

    Plasmid
    #206082
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E1658A
    Promoter
    CMV/B-actin
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    206082-plasmid-map-sequence-id-411174

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  7. Cav2.2 HA EIA (E314A) pCAGGS

    Plasmid
    #206081
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E314A
    Promoter
    CMV/B-actin
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    206081-plasmid-map-sequence-id-411173

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  8. Cav2.2 HA EIVK (E1658K) pcDNA3

    Plasmid
    #206066
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E1658K
    Promoter
    CMV
    Available since
    Oct. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    206066-plasmid-map-sequence-id-410269

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  9. Cav2.2 HA EIVK (E1658K) pCAGGS

    Plasmid
    #206096
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E1658K
    Promoter
    CMV/B-actin
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206096-plasmid-map-sequence-id-406757

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  10. TFORF1101

    Plasmid
    #143925
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GTF2IRD1 (GTF2IRD1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143925-plasmid-map-sequence-id-404588

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  11. TFORF1100

    Plasmid
    #143924
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GTF2IRD1 (GTF2IRD1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143924-plasmid-map-sequence-id-404587

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  16. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pCAGGS

    Plasmid
    #206115
    Purpose
    expression of rat Cav2.1 with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (Cacna1a Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E320A, E670A, E1411A, E1707A
    Promoter
    CMV/B-actin
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    206115-plasmid-map-sequence-id-415931

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  17. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pcDNA3

    Plasmid
    #206121
    Purpose
    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domains I, II, III and IV to to abolish divalent cation binding
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1a (Cacna1a Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E320A, E670A, E1411A, E1707A
    Promoter
    CMV
    Available since
    Oct. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    206121-plasmid-map-sequence-id-410287

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  19. pAAV hSyn INTRON mEmerald-ER WPRE

    Plasmid
    #236230
    Purpose
    AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum
    Depositor
    Jennifer Lippincott-Schwartz
    Article
    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do
    Insert
    mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)
    Use
    AAV
    Tags
    mEmerald
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    236230-plasmid-map-sequence-id-471247

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  20. GFP Cav2.2 human HAgHA -plus exon 18a - LONG-Cterm in pcDNA3

    Plasmid
    #198451
    Purpose
    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a- Long form of alternatively spliced C-teminus, in pcDNA3 vector
    Depositor
    Annette Dolphin
    Article
    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060
    Insert
    Cav2.2 (CACNA1B Human)
    Use
    Tags
    N-terminal GFP, internal double HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    198451-plasmid-map-sequence-id-392120

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Showing: 561 - 580 of 598 results