We narrowed to 1,028 results for: hug

1. NowGFP /pQE-30

   Plasmid

   #74749

   Purpose

   NowGFP gene for bacterial expression

   Depositor

   Konstantin Lukyanov

   Article

   Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

   Insert

   NowGFP
   

   Use

   Tags

   HisTag (with a mutation but still working)

   Expression

   Bacterial

   Mutation

   WasCFP with substitutions E6K, L42M, T43S, V68M, …

   Promoter

   T5

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiPGK Blast DEST ERKKTRmRuby2

   Plasmid

   #90231

   Purpose

   Lentiviral vector to express ERK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   ERK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR1a-tagRFP-KDEL

   Plasmid

   #114177

   Purpose

   Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

   Depositor

   Nicolas Demaurex

   Article

   Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

   Insert

   tagRFP-KDEL
   

   Use

   Gateway entry clone

   Tags

   KDEL retention signal and signal peptide

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTP261

   Plasmid

   #104166

   Purpose

   Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

   Depositor

   Dirk Görlich

   Article

   Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

   Insert

   Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
   

   Use

   Tags

   14x Histidine tag and NEDD8 from Brachypodium dis…

   Expression

   Bacterial

   Mutation

   1x Cysteine located at bps 493-495 in full sequen…

   Promoter

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. single polypeptide FPX biosensor for calcium ion

   Plasmid

   #60887

   Purpose

   FPX biosensor

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for calcium ion
   

   Use

   Tags

   HindIII site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDuEx2-NIR-GECO1

   Plasmid

   #113680

   Purpose

   Bacterial and mammalian expression plasmid for near-infrared fluorescent Ca2+ indicator NIR-GECO1

   Depositor

   Robert Campbell

   Article

   Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

   Insert

   NIR-GECO1
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiPGK Puro DEST ERKKTRClover

   Plasmid

   #90227

   Purpose

   Lentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   ERK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD-K-GECO1

   Plasmid

   #105864

   Purpose

   Bacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1

   Depositor

   Robert Campbell

   Article

   Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

   Insert

   K-GECO1
   

   Use

   Tags

   6-His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. BNLS

   Plasmid

   #50836

   Purpose

   Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddGFP B
   

   Use

   Tags

   triplicated NLS sequence DPKKKRKV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJC6-GLS-3xFLAG

    Plasmid

    #163450

    Purpose

    lentiviral overexpression vector for human GLS

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    GLS (GLS Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    See: comments

    Promoter

    Ubc

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. GANLS

    Plasmid

    #50852

    Purpose

    Expresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A
    

    Use

    Tags

    triplicated NLS sequence DPKKKRKV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. RANLS

    Plasmid

    #50843

    Purpose

    Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A
    

    Use

    Tags

    triplicated NLS sequence DPKKKRKV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE-hLin28B

    Plasmid

    #26358

    Depositor

    George Daley

    Article

    Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

    Insert

    lin-28 homolog B (LIN28B Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Nov. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiPGK Puro DEST JNKKTRmCerulean3

    Plasmid

    #90232

    Purpose

    Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    JNK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Seattle_IPDA_control_1_001

    Plasmid

    #167347

    Purpose

    ddPCR gating control for droplets containing all 5 targets (2 in pol)

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    pol_gag_tat_env
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. J23104-mRFP1-331Bb

    Plasmid

    #78274

    Purpose

    mRFP1 behind constitutive promoter J23104 in pSEVA331Bb backbone

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    J23104-mRFP1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Seattle_IPDA_control_14_004

    Plasmid

    #167350

    Purpose

    ddPCR gating control for droplets containing the env target

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    env
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Seattle_IPDA_control_32_007

    Plasmid

    #167353

    Purpose

    ddPCR gating control for droplets containing 5'pol or 3'pol targets

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    pol
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Seattle_IPDA_control_17_005

    Plasmid

    #167351

    Purpose

    ddPCR gating control for droplets containing gat and tat targets

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    gat_tat
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

    Plasmid

    #35501

    Purpose

    AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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