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pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-BFP)

Plasmid

#217305

Purpose

sgRNA BFP + Puromycin

Depositor

Luke Gilbert

Insert

pU6-sgRNA, Ef1alpha-Puro-T2A-BFP

Use

CRISPR and Synthetic Biology

Promoter

U6, Ef1alpha

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBS human c-Myc

Plasmid

#14971

Depositor

Joan Massague

Article

Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

Insert

c-Myc (MYC Human)

Expression

Bacterial

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pET28c-F30-Broccoli

Plasmid

#66788

Purpose

Expresses Broccoli in the F30 scaffold in bacterial cells

Depositor

Samie Jaffrey

Article

Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

Insert

Broccoli in the F30 scaffold

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pEVOL-AckRS

Plasmid

#137976

Purpose

sequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion

Depositor

Wenshe Liu

Article

Wang et al J Am Chem Soc. 2019 Feb 13;141(6):2462-2

Insert

AckRS and pylTcua

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Mfn1-Myc

Plasmid

#23212

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Mfn1 (Mfn1 Mouse)

Tags

6xHis, 9xMyc, and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pRMC2-msfGFP

Plasmid

#194913

Purpose

Tetracycline inducible expression of msfGFP in Staphylococci

Depositor

Rikke Meyer

Article

Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

Insert

Shine Dalgarno Sequence followed by monomeric superfolder GFP

Use

Tetracycline-inducible expression vector for stap…

Expression

Bacterial

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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PL-SIN-Oct4-EGFP

Plasmid

#21319

Purpose

Lentiviral vector, expresses EGFP under Oct3/4 promoter

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

Oct-4 promoter region

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-PBRM1

Plasmid

#65387

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

PBRM1 (PBRM1 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

Plasmid

#213973

Purpose

AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

Shank3 sgRNA

Use

AAV and CRISPR

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-hERG1a

Plasmid

#53052

Purpose

Expresses alpha subunit of hERG1a K channel in mammalian cells

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pRN111

Plasmid

#84463

Purpose

pJB38-NWMN29-30 + SarA_P1-mCherry-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-mCherry from pRN11

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-Ef1a-Blast

Plasmid

#227267

Purpose

Empty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.Flag.HERC2-HECT-WT.6xHis

Plasmid

#39227

Depositor

Aziz Sancar

Article

Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

Insert

HERC2 HECT Domain (HERC2 Human)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

T7

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pQE30-His-T7RNAP

Plasmid

#124138

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

T7 RNA polymerase (T7p07 Enterobacteria phage T7)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-MYC-IRES-lucOS

Plasmid

#129776

Purpose

Transposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339

Depositor

Amaia Lujambio

Article

Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

Insert

human MYC (MYC Human)

Tags

OS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKCH

Plasmid

#23765

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKCH (PRKCH Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLV-EF1a-CRISPRoffv2.1-IRES-BlastR

Plasmid

#207180

Purpose

Lentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a Promoter

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

CRISPRoffv2.1

Use

CRISPR and Lentiviral

Tags

HA and TagBFP

Expression

Mammalian

Promoter

EF1a

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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C-Terminal Split Cas9 with GyrA intein

Plasmid

#58694

Purpose

Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

Depositor

Gang Bao

Article

Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

Insert

Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein

Use

AAV and CRISPR

Tags

GyrA Csplit Intein and NLS

Expression

Mammalian

Promoter

CBh

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-Daxx/pRK5

Plasmid

#27974

Depositor

Xiaolu Yang

Article

Tang et al Nat Cell Biol. 2006 Aug . 8(8):855-62.

Insert

Daxx (DAXX Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only
pLminP_Luc2P_RE60

Plasmid

#247036

Purpose

Lentiviral expression of a minimal promoter only with single TATA cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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HA-TRIM28deltaPB

Plasmid

#124959

Purpose

Mammalian expression of Human TRIM28 with deleted PHD and BROMO domain

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of PHD and Bromo Domain

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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CTCF-mAC donor (Neo)

Plasmid

#140645

Purpose

CTCF tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CTCF (CTCF Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Venus-rab11a

Plasmid

#234744

Purpose

Mammalian expression of recycling endosome marker Venus-rab11a

Depositor

Nevin Lambert

Article

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Insert

Venus-rab11a (RAB11A Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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mitoZFD_ND1-Right-G1397-C

Plasmid

#180769

Purpose

Expresses mitoZFD_ND1-Right-G1397-C(DddAtox half) in mammalian cells

Depositor

Jin-Soo Kim

Article

Lim et al Nat Commun. 2022 Jan 18;13(1):366. doi:

Insert

mitoZFD for ND1 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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mitoZFD_ND1-Left-G1397-N

Plasmid

#180768

Purpose

Expresses mitoZFD_ND1-Left-G1397-N(DddAtox half) in mammalian cells

Depositor

Jin-Soo Kim

Article

Lim et al Nat Commun. 2022 Jan 18;13(1):366. doi:

Insert

mitoZFD for ND1 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA2-CAND1

Plasmid

#20719

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)

Tags

HA2

Expression

Mammalian

Available Since

April 21, 2009

Availability

Academic Institutions and Nonprofits only
pET32a-hEGF

Plasmid

#199231

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Epidermal growth factor, partial (EGF Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pIVM_26

Plasmid

#204501

Purpose

Expression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)

Depositor

Alessio Ciulli

Article

Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

Insert

EloB (1-104) and EloC (17-112) (ELOC, ELOB Human)

Expression

Bacterial

Mutation

0

Promoter

AmpR

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pINDUCER20_mStrawberry

Plasmid

#161734

Purpose

Doxycycline-inducible expression of mStrawberry

Depositor

Alec Kimmelman

Article

Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

Insert

mStrawberry

Use

Lentiviral; Destinatioin vector for gateway cloni…

Expression

Mammalian

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-R-diLACCO1

Plasmid

#208028

Purpose

Control biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-diLACCO1

Expression

Mammalian

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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