313,983 results

1. pGP-AAV-syn-jGCaMP8s-WPRE

   Plasmid

   #162374

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8s
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S26M F286Y Q315H

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG

   Plasmid

   #225242

   Purpose

   Mammalian expression of iPASnFR-L in the cytosol

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-L
   

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mApple-N1

   Plasmid

   #54567

   Purpose

   Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Type

   Empty backbone

   Tags

   mApple

   Expression

   Mammalian

   Available Since

   July 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. mApple-N1

   Plasmid

   #54567

   Purpose

   Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Type

   Empty backbone

   Tags

   mApple

   Expression

   Mammalian

   Available Since

   July 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-Pol-II WT

   Plasmid

   #35175

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al RNA. 2004 Apr;10(4):581-9.

   Insert

   RPBI (POLR2A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-Pol-II WT

   Plasmid

   #35175

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al RNA. 2004 Apr;10(4):581-9.

   Insert

   RPBI (POLR2A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA GSK3 beta K85A pcDNA3

   Plasmid

   #14755

   Depositor

   Jim Woodgett

   Article

   Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

   Insert

   GSK3 beta K85A (GSK3B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K85A. Kinase dead mutant.

   Available Since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. HA GSK3 beta K85A pcDNA3

   Plasmid

   #14755

   Depositor

   Jim Woodgett

   Article

   Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

   Insert

   GSK3 beta K85A (GSK3B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K85A. Kinase dead mutant.

   Available Since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)

   Plasmid

   #225752

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. emiRFP670-cGAS

    Plasmid

    #231896

    Purpose

    Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    cGAS (CGAS Human)
    

    Tags

    emiRFP670

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti6.2_3xFLAG_V5_ mmIfih1_V5

    Plasmid

    #160110

    Purpose

    Expresses murine Ifih1 in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Ifih1 (Ifih1 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG, V5 and V5

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti6.2_3xFLAG_V5_ mmIfih1_V5

    Plasmid

    #160110

    Purpose

    Expresses murine Ifih1 in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Ifih1 (Ifih1 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG, V5 and V5

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. AbVec1.1-IGLC2-XhoI

    Plasmid

    #99575

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. AbVec1.1-IGLC2-XhoI

    Plasmid

    #99575

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-alphasynuclein-WT

    Plasmid

    #40822

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-alphasynuclein-WT

    Plasmid

    #40822

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCherry.90 alpha

    Plasmid

    #108222

    Purpose

    Mammalian expression vector for mCherry fusion to human Hsp90 alpha

    Depositor

    Didier Picard

    Article

    Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

    Insert

    mCherry-Hsp90a (HSP90AA1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCherry.90 alpha

    Plasmid

    #108222

    Purpose

    Mammalian expression vector for mCherry fusion to human Hsp90 alpha

    Depositor

    Didier Picard

    Article

    Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

    Insert

    mCherry-Hsp90a (HSP90AA1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBABE

    Plasmid

    #21836

    Depositor

    David Ron

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    farnysylation signal

    Expression

    Mammalian

    Available Since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE

    Plasmid

    #21836

    Depositor

    David Ron

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    farnysylation signal

    Expression

    Mammalian

    Available Since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1-Cyto-iPASnFR-H-mScarlet3-3xFLAG

    Plasmid

    #225241

    Purpose

    Mammalian expression of iPASnFR-H in the cytosol

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-H
    

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pSMAL-CellTag-V1

    Pooled Library

    #115643

    Purpose

    Viral barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics

    Depositor

    Samantha Morris

    Article

    Biddy et al Nature. 2018 Dec;564(7735):219-224. doi:

    Has Service

    Concentrated Lentiviral Prep

    Expression

    Mammalian

    Species

    Synthetic

    Use

    Lentiviral

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

23. pBR BsmAIM Kan + p15A BsaIR Amp

    Plasmid

    #206208

    Purpose

    Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    BsamAIM and BsaIR
    

    Expression

    Bacterial

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pBR BsmAIM Kan + p15A BsaIR Amp

    Plasmid

    #206208

    Purpose

    Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    BsamAIM and BsaIR
    

    Expression

    Bacterial

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mCherry-Rab7a-7

    Plasmid

    #55127

    Purpose

    Localization: Ras GTPase, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Rab7a (RAB7A Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    N117S in NM_004637.5

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mCherry-Rab7a-7

    Plasmid

    #55127

    Purpose

    Localization: Ras GTPase, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Rab7a (RAB7A Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    N117S in NM_004637.5

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDx_mScarlet-I3

    Plasmid

    #189757

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I3
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDx_mScarlet-I3

    Plasmid

    #189757

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I3
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Syn61Δ3(ev5)

    Bacterial Strain

    #174514

    Purpose

    Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Bacterial Resistance

    Streptomycin

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

30. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid

    #198468

    Purpose

    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

    Depositor

    Viviana Risca

    Article

    Soroczynski et al bioRxiv 2024.07.11.602973

    Insert

    10His-pG-Tn5 (E54K, L372P)
    

    Tags

    10His-pG

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    Consensus φ10 T7 Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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