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pJL3

Plasmid

#184131

Purpose

For rescuing null mrp-1 mutation and labelling mrp-1 protein. Intestinal specific Pges-1 promoter drives MRP-1 expression. Translational fusion construct. pPD95.75_Pges-1::mrp-1(isoform C cDNA)::gfp.

Depositor

Alejandro Aballay

Article

Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

Insert

MRP-1 (mrp-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Pges-1 (3.3kb)

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-SwitchON_mRubyNLS-hChR2(H134R)-EYFP-NO_WPRE

Plasmid

#118279

Purpose

Expresses mRuby2 in the nucleus of Cre-negative cells, and ChR(H134R)-YFP in Cre-positive cells

Depositor

Dietmar Schmitz

Article

Wozny et al Front Cell Neurosci. 2018 Oct 2;12:337.

Inserts

mRuby2
ChR2(H134R)

Use

AAV and Cre/Lox

Tags

SV40 NLS and YFP

Promoter

EF1 alpha

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti.hUbC.H2B-CeruleanFP-2A-Dendra2FP.W

Plasmid

#126522

Purpose

Lentivector that expresses H2B-Cerulean and Dendra2 fluorescent proteins from an internal hUbC promoter.

Depositor

Rusty Lansford

Article

Huss et al Front Cell Dev Biol. 2019 Mar 28;7:35. d

Insert

H2B-CeruleanFP-2A-Dendra2FP

Use

Lentiviral

Promoter

hUbC

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG3_F(ab')2

Plasmid

#105860

Purpose

Expression plasmid coding for heavy chain of mouse F(ab')2 of IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG3 F(ab')2 (Ighg3 Mouse)

Expression

Mammalian

Promoter

hEF1-HTLV prom

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Vimentin-7

Plasmid

#55452

Purpose

Localization: Intermediate Filaments, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Vimentin (VIM Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
AtS/MAR10 insulator sequence (GB3458)

Plasmid

#160638

Purpose

pEGB3a1 AtS/MAR10

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

AtS/MAR10 insulator sequence

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pB80-SSPB(micro)-GFP-ppKin14VIb(861-1321)

Plasmid

#174639

Purpose

Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

SSPB(micro)-GFP-ppKinVIb(861-1321)

Tags

SSPB(micro)-GFP

Expression

Mammalian

Mutation

SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…

Promoter

Chicken beta-actin

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE_puro_ZFP64_N_3XFLAG

Plasmid

#118693

Purpose

Express 3xflag tagged ZFP64 for flag ChIP experiments

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

ZFP64 (ZFP64 Human)

Use

Lentiviral

Promoter

CMV

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_WT_ZFP64

Plasmid

#118661

Purpose

Express human wild type ZFP64 gene

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

ZFP64 (ZFP64 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pSB165 - pL1R3_pMAS::rLUC-I::tMAS

Plasmid

#123198

Purpose

binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

pMAS::rLUC-I::tMAS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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mTFP1-Vimentin-7

Plasmid

#55515

Purpose

Localization: Intermediate Filaments, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Vimentin (VIM Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mPapaya1-Vimentin-7

Plasmid

#56654

Purpose

Localization: Intermediate Filaments, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Vimentin (VIM Human)

Tags

mPapaya1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAP2

Plasmid

#41755

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc Fly, E. coli)

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C45S C338S K349C C451S

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2-sgSORCS2-G2

Plasmid

#118416

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting Human SORCS2

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA SORCS2 Human (SORCS2 Human)

Use

CRISPR and Lentiviral

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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rAAV-Ef1α-EGFP-WPRE-ID-PolyA

Plasmid

#241829

Purpose

AAV construct for constitutive expression of EGFP. Contains a molecular barcode for identification of AAV genome using single-cell sequencing.

Depositor

Benjamin Arenkiel

Article

Belfort et al Front Neurosci. 2025 Feb 13;19:1531122.

Insert

EGFP-WPRE-ID-PolyA

Use

AAV

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET28:LanIV-Streptomyces

Plasmid

#226250

Purpose

Expresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIV-Streptomyces)

Tags

His6

Expression

Bacterial

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-56

Plasmid

#225073

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-56)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2C

Plasmid

#225070

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2C)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2A

Plasmid

#225068

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2A (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2A)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2B

Plasmid

#225069

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2B (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2B)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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