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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,282 results for: LEA

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Showing: 5781 - 5800 of 12282 results

  4. pMT899

    Plasmid
    #228288
    Purpose
    Constitutive mutant rPhlTA expression with strong promoter
    Depositor
    Jun Ishii
    Publication
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    rphlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    P5S, S6P, K86T, F109L, Q117R, E143K
    Promoter
    KpGAPDH promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228288-plasmid-map-sequence-id-457110

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  6. pETSUMO2_Nostoc-bGSDM-WELQ

    Plasmid
    #222100
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Publication
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Nostoc-bGSDM-WELQ ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222100-plasmid-map-sequence-id-437100

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  8. pBactin-Nedd1-mOrange2-F75A

    Plasmid
    #196862
    Purpose
    Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-F75A (Nedd1 Rat)
    Expression
    Mammalian
    Mutation
    F75A in the CM1 domain
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196862-plasmid-map-sequence-id-391831

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  10. pcDNA3-Dronpa-Kras

    Plasmid
    #182329
    Purpose
    Plasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Publication
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dronpa-Kras
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    182329-plasmid-map-sequence-id-363283

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  11. pTox-EGFPsite1_NGG-PAM (BPK740)

    Plasmid
    #181746
    Purpose
    p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGG PAM
    Depositor
    Benjamin Kleinstiver
    Publication
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)
    Use
    Selection plasmid
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181746-plasmid-map-sequence-id-358235

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  13. pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA

    Plasmid
    #178868
    Purpose
    Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVcs-mTagBFP-HA
    Depositor
    Shinya Tsukiji
    Publication
    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    178868-plasmid-map-sequence-id-355800

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  14. pCI-Flag-NEDD4L∆WW2

    Plasmid
    #166708
    Purpose
    expresses NEDD4L with inactivating mutation in second WW domain in mammalian cells
    Depositor
    Wesley Sundquist
    Publication
    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NEDD4L W453A
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed tryptophan 453 to alanine
    Promoter
    CMV promoter
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166708-plasmid-map-sequence-id-329114

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  15. pCI-Flag-NEDD4L∆WW3

    Plasmid
    #166709
    Purpose
    expresses NEDD4L with inactivating mutation in third WW domain in mammalian cells
    Depositor
    Wesley Sundquist
    Publication
    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NEDD4L W525A
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed tryptophan 525 to alanine
    Promoter
    CMV promoter
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166709-plasmid-map-sequence-id-329115

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  19. pGBW-m4133478

    Plasmid
    #152804
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp4
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152804-plasmid-map-sequence-id-317517

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  20. pGBW-m4134407

    Plasmid
    #152787
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp10
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152787-plasmid-map-sequence-id-317515

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Showing: 5781 - 5800 of 12282 results