We narrowed to 19,248 results for: Tat

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pICSL50006

Plasmid

#50319

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, luciferase (Photinus pyralis)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-enCjCas9-BlastR

Plasmid

#220978

Purpose

Expresses human codon-optimized enCjCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

enCjCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shControl

Plasmid

#180386

Purpose

Producing AAV that encodes negative control shRNA with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shControl

Use

AAV and RNAi

Promoter

CBA

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTrcHIS-7D12Gln13TAG

Plasmid

#125264

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

Gln13TAG 7D12

Tags

His-Tag

Expression

Bacterial

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCDB328

Plasmid

#113672

Purpose

expresses Ulp1_R4 in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R4

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pWT055e

Plasmid

#96866

Purpose

sgRNA-EMX1

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

sgRNA-EMX1

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLK88 - Edit-directing plasmid base

Plasmid

#98814

Purpose

Edit-directing plasmid base

Depositor

Leonid Kruglyak

Insert

CAN1.y gRNA

Use

CRISPR

Expression

Yeast

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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PK187

Plasmid

#44623

Depositor

Robert Oshima

Article

Oshima et al Differentiation. 1986;33(1):61-8.

Insert

K18 (KRT18 Human)

Use

Cloning

Available Since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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pRL-TK let7 C

Plasmid

#11326

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation C from figure 4A of paper.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 I

Plasmid

#11332

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation I from figure 4A of paper.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pET-6xHis-GFP(+6)

Plasmid

#199160

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+6)

Tags

MGHHHHHHGGA

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP720-HO1

Plasmid

#197257

Purpose

Expresses the protein of miRFP720 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP720

Expression

Bacterial

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP680-HO1

Plasmid

#197253

Purpose

Expresses the protein of miRFP680 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP680

Expression

Bacterial

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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UAS-u(S)Cas9

Plasmid

#127383

Purpose

Expresses Cas9 at high level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(S)-Cas9

Use

CRISPR

Expression

Insect

Promoter

UAST

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pWT016i

Plasmid

#96856

Purpose

theophylline-agRNA-GFP

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

theophylline-agRNA-GFP

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DCAF8_WT_V5

Plasmid

#82947

Purpose

Gateway Donor vector containing DCAF8, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DCAF8 (DCAF8 Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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PB-Gata2

Plasmid

#60665

Purpose

PiggyBac expression vector encoding rat Gata2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Gata2 (Gata2 Rat)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pNR230

Plasmid

#79546

Purpose

BxbI attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ159

Plasmid

#69308

Purpose

Gateway entry vector with hSpCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

hSpCas9D10A (Human codon optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

D10A mutation; NLS fusion

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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MP-P9

Plasmid

#69668

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR ugi APOBEC1

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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