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pNLF1-N_DCAF1:1038-1400

Plasmid

#210962

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_FBXW11:1-542

Plasmid

#210952

Purpose

Mammalian expression of full-length human FBXW11. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXW11:M1-R542 (FBXW11 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_SKIC8:1-305

Plasmid

#211038

Purpose

Mammalian expression of full-length human SKIC8. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SKIC8:M1-I305 (SKIC8 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_COPB2:1-597

Plasmid

#211055

Purpose

Mammalian expression of human COPB2 fragment M1 to A597. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

COPB2:M1-A597 (COPB2 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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Mouse sphingolipid metabolism knockout library

Pooled Library

#206249

Purpose

The sphingolipid metabolism library targets 82 mouse genes in the de novo ceramide and downstream sphingolipid metabolism pathways.

Depositor

Kivanc Birsoy

Article

Soula et al Nature. 2024 Aug 7. doi: 10.1038/s41586-

Use

CRISPR and Lentiviral

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only
pHAGE-ERBB3

Plasmid

#116735

Purpose

Lentiviral expression of ERBB3

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB3 (ERBB3 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-IDH1-R132C

Plasmid

#116410

Purpose

Lentiviral expression of IDH1 R132C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

IDH1 (IDH1 Human)

Use

Lentiviral

Mutation

R132C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-IDH1

Plasmid

#116751

Purpose

Lentiviral expression of IDH1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

IDH1 (IDH1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB3-V104M

Plasmid

#116363

Purpose

Lentiviral expression of ERBB3 V104M

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB3 (ERBB3 Human)

Use

Lentiviral

Mutation

V104M

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB3-D297Y

Plasmid

#116360

Purpose

Lentiviral expression of ERBB3 D297Y

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB3 (ERBB3 Human)

Use

Lentiviral

Mutation

D297Y

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB3-G40S

Plasmid

#116361

Purpose

Lentiviral expression of ERBB3 G40S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB3 (ERBB3 Human)

Use

Lentiviral

Mutation

G40S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-IDH1-R132L

Plasmid

#116411

Purpose

Lentiviral expression of IDH1 R132L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

IDH1 (IDH1 Human)

Use

Lentiviral

Mutation

R132L

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-IDH1-P33S

Plasmid

#116409

Purpose

Lentiviral expression of IDH1 P33S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

IDH1 (IDH1 Human)

Use

Lentiviral

Mutation

P33S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Taq DNA Pol

Plasmid

#178782

Purpose

Inducible expression of Taq DNA polymerase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Polymerase

Expression

Bacterial

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiPGK DEST H2B-iRFP670

Plasmid

#90237

Purpose

Lentiviral vector to express H2B-iRFP670 under PGK promoter

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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5HT6-mCherry-G-GECO1.0

Plasmid

#47500

Purpose

Fluorescent reporter for Ca2+ inside primary cilia with mCherry reference

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

G-GECO1.0

Tags

5HT6 and mCherry

Expression

Mammalian

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pPBCAG-rtTAM2-IH

Plasmid

#140827

Purpose

Expresses rtTAM2-IRES-HygR in mammalian cells

Depositor

Hideyuki Okano

Article

Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

Inserts

rtTAM2
HygR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Taq DNA Ligase

Plasmid

#178798

Purpose

Inducible expression of Taq DNA Ligase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Ligase

Expression

Bacterial

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL WS1.6 WASp

Plasmid

#36250

Depositor

David Rawlings

Article

Astrakhan et al Blood. 2012 Mar 19.

Insert

WASp (WAS Human)

Use

Lentiviral

Promoter

WS1.6

Available Since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

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pSF11-wElectra2

Plasmid

#184933

Purpose

Codon optimized blue fluorescent protein Electra2 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra2

Expression

Worm

Promoter

tag-168

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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