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pTBRII / CEP-Zeo / Hygro

Plasmid

#16622

Depositor

Bert Vogelstein

Article

Zhou et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Insert

TGF beta type II receptor (TGFBR2 Human)

Tags

HA

Expression

Mammalian

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-ArgiNLS-EGFP

Plasmid

#220594

Purpose

Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

EGFP

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-nEF-dCas9

Plasmid

#106430

Purpose

Expresses SpdCas9 from the nEF promoter in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

nEF-dCas9

Use

AAV and CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Promoter

nEF

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-nef-CIAO2-Flp

Plasmid

#149296

Purpose

Cre-dependent expression of Flp recombinase.

Depositor

Edward Callaway

Article

Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

Insert

Flp recombinase (flp Budding Yeast)

Use

AAV

Mutation

Derived from the mouse codon-optomized FlpO. The…

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV N-term HA-FLAG puro BRD7

Plasmid

#41934

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

HA-Flag

Expression

Mammalian

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
Chr1q_Centromere-Targeting_gRNA

Plasmid

#195125

Purpose

gRNA targeting centromere-proximal location on Chromosome 1q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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J23104-mRFP1-331Bb

Plasmid

#78274

Purpose

mRFP1 behind constitutive promoter J23104 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23104-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC S62D

Plasmid

#203437

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Mutation

S62D

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV4Neo-murine IL-17RA-HA

Plasmid

#46859

Purpose

expresses mouse IL-17RA

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA (Il17ra Mouse)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pLM-mCitrine-Sox2

Plasmid

#23242

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCitrine_2A_SOX2 (SOX2 Human, synthetic fluorescent protein)

Use

Lentiviral

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pHD-T2A-split-intein-Gal4[21-881]-C-int

Plasmid

#199204

Purpose

To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-nef-CIAO-Flp

Plasmid

#149294

Purpose

Cre-dependent expression of Flp recombinase.

Depositor

Edward Callaway

Article

Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

Insert

Flp recombinase (flp Budding Yeast)

Use

AAV

Mutation

Derived from the mouse codon-optomized FlpO. The…

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4-ATR-C-KD

Plasmid

#53770

Purpose

mammalian expression of flag-tagged ATR C-terminal fragment kinase dead mutant

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

Insert

ATR (ATR Human)

Tags

Flag

Expression

Mammalian

Mutation

C-terminal domain, aa 1398-2644, and kinase dead …

Promoter

CMV

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only
pET15b+His SUMO PPM1H

Plasmid

#207333

Purpose

Bacterial expression of His SUMO PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

His-SUMO

Expression

Bacterial

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMax_mPlum_C207G/T208C

Plasmid

#177831

Purpose

Site-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mPlum_C207G/T208C

Expression

Mammalian

Mutation

C207G + T208C

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pQ-NFY-A-GB-HC

Plasmid

#171081

Purpose

Co-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYC

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Inserts

NF-YA (NFYA Human)
NF-YB (NFYB Human)
NF-YC (NFYC Human)

Tags

GST and His

Expression

Bacterial

Promoter

T5 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-hLIN28A

Plasmid

#47902

Purpose

Expresses human LIN28A in mammalian cells using retrovirus system.

Depositor

Shinya Yamanaka

Article

Tanabe et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

human LIN28A (LIN28A Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 2, 2013

Availability

Academic Institutions and Nonprofits only

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904 pLNCX HA Akt1 K179M

Plasmid

#9007

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 K179M (AKT1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

K179M. Kinase dead.

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pAPM-miR30-DDX58-ts3

Plasmid

#174243

Purpose

DDX58 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

DDX58 shRNA (RIGI Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV4Neo-murine IL-17RC(FL)

Plasmid

#46864

Purpose

expresses mouse IL-17RC (all exons)

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RC (Il17rc Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only

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