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SL2-sgTelo-MTSa/BFP/pdCas9-C1

Plasmid

#162760

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting telomeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgTelo-MTSa)

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-mRuby3-2A-dCre

Plasmid

#203845

Purpose

Flp-dependent enzymatically inactive Cre and mRuby3 expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dCre

Use

AAV

Tags

mRuby3-2A

Mutation

Enzymatically inactive Cre

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCSN061

Plasmid

#101725

Purpose

Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

Depositor

Rene Verwaal

Article

Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

Inserts

S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
KanMX marker expression cassette.

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Promoter

Heterologous TEF1 promoter from A. gossypii. and …

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pUD628

Plasmid

#103018

Purpose

expression of a Cpf1 programming crRNA targetting ADE2 (crADE2-3.S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crADE2-3.S (ADE2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Pb459-mU6-sgRNA-EF1a-PuroR

Plasmid

#195507

Purpose

piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

Puromycin

Use

CRISPR; Piggybac

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_IDH2

Plasmid

#183301

Purpose

All-in-One CRISPRko system with a guide RNA that targets IDH2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

IDH2

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pFlox_N-p300_Neo_FKBPF36V_mScarlet

Plasmid

#163886

Purpose

Acute Depletion of P300-mScarlet

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

P300 (Ep300 Mouse)

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-EGFR-T790M-tmpknot-epegRNA

Plasmid

#214093

Purpose

Lentiviral vector expressing epegRNA to induce EGFR T790M mutation

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

EGFR T790M epegRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-ePEG-LINE1-loxPsym

Plasmid

#216866

Purpose

Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements

Depositor

Leopold Parts

Article

Koeppel et al Science. 2025 Jan 31;387(6733):eado3979.

Insert

spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IBU-PFAS-FLAG

Plasmid

#168226

Purpose

Expresses human PFAS-3xFLAG fusion in mammalian cells

Depositor

Noboru Mizushima

Article

Mimura et al J Biol Chem. 2021 Jan-Jun;296:100780. do

Insert

PFAS (PFAS Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pHeCAHS-mEGFP-NLS

Plasmid

#205014

Purpose

The vector contains 1kbp of the upstream and downstream regions of the chas gene (BV898_02951, CAHS 86272) from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

Depositor

Kazuharu Arakawa

Article

Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

Insert

mEGFP-NLS flanked by 1kbp upstream and downstream of the chas gene from H. exemplaris

Use

Tardigrade expression

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pFlox c-CTCF FKBPF36V_EYFP_p2aBsd

Plasmid

#163870

Purpose

Acute Depletion of CTCF-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Ctcf (Ctcf Mouse)

Expression

Mammalian

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pFlox_N-RPB1_BSD_FKBPF36V_EYFP

Plasmid

#163884

Purpose

Acute Depletion of RBP1-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Rbp1 (Polr2a Mouse)

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD_A0601

Plasmid

#91016

Purpose

Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_D10A nickase

Use

CRISPR

Mutation

D10A

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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CD200RCd4d3+4-bio

Plasmid

#32406

Purpose

A positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200 receptor) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200 Receptor extracellular domain (Cd200r1 Rat)

Tags

Cd4d3+4 and biotinylation peptide

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_MYC

Plasmid

#183330

Purpose

All-in-One CRISPRko system with a guide RNA that targets MYC gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

MYC

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJG380

Plasmid

#91188

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, nuclease (AtCas9+gNt_R2+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9+gNt_R2+donor

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pQTEV-GYG

Plasmid

#31324

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

glycogenin (GYG1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pSCL.028

Plasmid

#184965

Purpose

Test effect of extending -Eco1 a1/a2 in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RT and ncRNA(extended), a1/a2 length: 27

Expression

Yeast

Mutation

human codon optimized RT, a1/a2 length extended t…

Promoter

Gal7

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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RFN_EGFP_81

Plasmid

#60073

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only