We narrowed to 18,052 results for: ERG

1. pGEX-4T1-Rac1 G15A

   Plasmid

   #69355

   Purpose

   expression of nucleotide free Rac1 in bacterial cells

   Depositor

   Rafael Garcia-Mata

   Article

   Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

   Insert

   Rac1 (RAC1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   G15A

   Promoter

   tac

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET51b(+)_SNAP-p24

   Plasmid

   #130718

   Purpose

   Plasmid encoding the bacterial expression of a SNAP-fusion of p24 HIV Capsid protein

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   SNAP-Tag-p24
   

   Tags

   Hisx10 and StrepTag

   Expression

   Bacterial

   Mutation

   Deleted first Met, Ala and Last Ser from the Enha…

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. FOR human NANOS3 TALEN

   Plasmid

   #59722

   Purpose

   TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

   Depositor

   Jacob Hanna

   Article

   Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

   Insert

   HD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Dec. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

4. REV human NANOS3 TALEN

   Plasmid

   #59723

   Purpose

   TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

   Depositor

   Jacob Hanna

   Article

   Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

   Insert

   NG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Dec. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. 2XMyc-LRRK2-WD40

   Plasmid

   #25073

   Depositor

   Mark Cookson

   Article

   J Biol Chem. 2008 Jun 13;283(24):16906-1

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   2XMyc

   Expression

   Mammalian

   Mutation

   Contains only the WD40 domain aa 2155-2527

   Available Since

   Nov. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pDEST51-LRRK2-R1441G

   Plasmid

   #25082

   Depositor

   Mark Cookson

   Article

   J Biol Chem. 2008 Jun 13;283(24):16906-1

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Destination vector

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   Arginine 1441 mutated to Glycine

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

7. p3E-ING2

   Plasmid

   #109554

   Purpose

   Multisite gateway vector for 3' tagging with 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. actc1b-mKate2-Bin1b

   Plasmid

   #109509

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to Bin1b

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Bin1b (bin1b Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AA345

   Plasmid

   #216031

   Purpose

   Fragmid fragment: (Cas protein) Cas13d

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas13d_v1.1 [Dj]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDA_834

    Plasmid

    #216087

    Purpose

    Cas9 [Sp] knockout targeting CD59, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD59 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-syn-SnFR-gamma8-minWPRE

    Plasmid

    #165498

    Purpose

    AAV for glutamate reporter fused to TARP gamma-8

    Depositor

    Andrew Plested

    Article

    Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

    Insert

    iGluSnFR extracellular domain fused to TARP gamma-8 via NETO2 TM domain (Cacng8 Synthetic, Rat)
    

    Use

    AAV

    Promoter

    hSynapsin

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. SaLgCP

    Plasmid

    #164562

    Purpose

    Puro

    Depositor

    Junwei Shi

    Article

    Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Flag-NLS-SaCas9-P2A-PuroR

    Expression

    Mammalian

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-tomato-TSC2-sgRNA

    Plasmid

    #196195

    Purpose

    Editing human TSC2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    TSC2 (TSC2 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK5-mFzd8-1D4

    Plasmid

    #42270

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 8 (Fzd8 Mouse)
    

    Tags

    1D4

    Expression

    Mammalian

    Available Since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pMTH PKC zeta

    Plasmid

    #8414

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Mouse Protein Kinase C zeta (Prkcz Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

16. actc1b-mKate2-FAPP1

    Plasmid

    #109503

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4P

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-mKate2-FAPP1
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pY012 (pcDNA3.1-hCMtCpf1)

    Plasmid

    #69984

    Purpose

    Expresses humanized CMtCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hCMtCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-6017nes

    Plasmid

    #108655

    Purpose

    A PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-PKA-EV
    

    Tags

    XFP

    Expression

    Mammalian

    Promoter

    pCAGGS

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. actc1b-mKate2-2xFYVEhgs

    Plasmid

    #109505

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to mouse 2x PH domain from HGF regulated tyrosine kinase substrate (Hgs). Binds PtdIns3P

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-mKate2-2xFYVEhgs
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 2XMyc-LRRK2-LRRs

    Plasmid

    #25072

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Contains only the LRR domains aa879-1278

    Available Since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only